Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Lipid A deacylase PagL

Gene

pagL

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has lipid A 3-O-deacylase activity. Hydrolyzes the ester bond at the 3 position of lipid A, a bioactive component of lipopolysaccharide (LPS), thereby releasing the primary fatty acyl moiety. Lacks fatty acyl chain-length specificity as removes both 3-OH C10 and 3-OH C14 fatty acids from lipid A.3 Publications

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.3 Publications

Enzyme regulationi

Decreased activity at low temperatures (15 or 21 degrees Celsius).1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei149 – 1491Charge relay system1 Publication
Active sitei151 – 1511Charge relay system1 Publication
Sitei152 – 1521Critical for activity1 Publication
Active sitei163 – 1631Charge relay system1 Publication

GO - Molecular functioni

  • acyloxyacyl hydrolase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • lipid A metabolic process Source: UniProtKB
  • lipopolysaccharide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A deacylase PagL1 PublicationImported (EC:3.1.1.773 Publications)
Alternative name(s):
LPS 3-O-deacylase PagL1 Publication
Outer membrane enzyme PagL1 Publication
PhoP/PhoQ-activated gene product L1 Publication
Gene namesi
Name:pagLImported
Ordered Locus Names:PA4661
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4661.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 284Periplasmic1 Publication
Transmembranei29 – 324Beta stranded1 Publication
Topological domaini33 – 331Periplasmic1 Publication
Transmembranei34 – 4916Beta stranded1 PublicationAdd
BLAST
Topological domaini50 – 567Extracellular1 Publication
Transmembranei57 – 7115Beta stranded1 PublicationAdd
BLAST
Topological domaini72 – 732Periplasmic1 Publication
Transmembranei74 – 8916Beta stranded1 PublicationAdd
BLAST
Topological domaini90 – 901Extracellular1 Publication
Transmembranei91 – 933Beta stranded1 Publication
Topological domaini94 – 952Periplasmic1 Publication
Transmembranei96 – 983Beta stranded1 Publication
Topological domaini99 – 1002Extracellular1 Publication
Transmembranei101 – 11515Beta stranded1 PublicationAdd
BLAST
Topological domaini116 – 1172Periplasmic1 Publication
Transmembranei118 – 12811Beta stranded1 PublicationAdd
BLAST
Topological domaini129 – 13810Extracellular1 Publication
Transmembranei139 – 14810Beta stranded1 Publication
Topological domaini149 – 17325Periplasmic1 PublicationAdd
BLAST

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Pharmaceutical usei

Might be useful for the development of new vaccines or adjuvants because of its LPS-modifying properties. May be used for the design of inhibitors with possible therapeutic value.1 Publication

Disruption phenotypei

Has similar growth characteristics to wild-type in LB medium at different temperatures (25, 30 or 37 degrees Celsius) or in LB medium supplemented with 0-4 M sodium chloride, 0-1,500 µg/ml chloramphenicol, 0-5% ethanol or 0-1% chloroform. Only when chloramphenicol is present in the medium is a difference in the ability to grow observed. The difference is most pronounced at a concentration of 650 µg/ml chloramphenicol, where the absorbance of the wild-type culture after overnight growth is 1.7-fold higher than that of the pagL disruption mutant.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi81 – 811H → A or N: Does not affect LPS deacylation activity. 1 Publication
Mutagenesisi84 – 841S → A or C: Does not affect LPS deacylation activity. 1 Publication
Mutagenesisi149 – 1491H → A or N: No deacylation of LPS. 1 Publication
Mutagenesisi151 – 1511S → A or C: No deacylation of LPS. 1 Publication
Mutagenesisi152 – 1521N → A: Inactive. 1 Publication
Mutagenesisi159 – 1591N → A: 304-fold reduction in activity. 1 Publication
Mutagenesisi163 – 1631E → A: 142-fold reduction in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysis1 PublicationAdd
BLAST
Chaini24 – 173150Lipid A deacylase PagLSequence analysis1 PublicationPRO_0000422912Add
BLAST

Proteomic databases

PaxDbiQ9HVD1.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi208964.PA4661.

Structurei

Secondary structure

1
173
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi25 – 328Combined sources
Beta strandi38 – 4710Combined sources
Beta strandi52 – 554Combined sources
Beta strandi58 – 7114Combined sources
Beta strandi78 – 9316Combined sources
Beta strandi96 – 11621Combined sources
Beta strandi124 – 13714Combined sources
Beta strandi142 – 15110Combined sources
Beta strandi162 – 17211Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ERVX-ray2.00A/B24-173[»]
ProteinModelPortaliQ9HVD1.
SMRiQ9HVD1. Positions 24-173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HVD1.

Family & Domainsi

Sequence similaritiesi

Belongs to the PagL family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG41061N2. Bacteria.
ENOG4112AQW. LUCA.
HOGENOMiHOG000264842.
KOiK12976.
OMAiQPNDGVE.

Family and domain databases

Gene3Di2.40.160.20. 1 hit.
InterProiIPR018550. Lipid-A_deacylase-rel.
IPR011250. OMP/PagP_b-brl.
[Graphical view]
PfamiPF09411. PagL. 1 hit.
[Graphical view]
PIRSFiPIRSF029681. PagL. 1 hit.
SUPFAMiSSF56925. SSF56925. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HVD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLLPLAVL AALSSVHVAS AQAADVSAAV GATGQSGMTY RLGLSWDWDK
60 70 80 90 100
SWWQTSTGRL TGYWDAGYTY WEGGDEGAGK HSLSFAPVFV YEFAGDSIKP
110 120 130 140 150
FIEAGIGVAA FSGTRVGDQN LGSSLNFEDR IGAGLKFANG QSVGVRAIHY
160 170
SNAGLKQPND GIESYSLFYK IPI
Length:173
Mass (Da):18,394
Last modified:March 1, 2001 - v1
Checksum:i3B557C5787CD7B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08048.1.
PIRiF83062.
RefSeqiNP_253350.1. NC_002516.2.
WP_003099307.1. NZ_ASJY01000743.1.

Genome annotation databases

EnsemblBacteriaiAAG08048; AAG08048; PA4661.
GeneIDi881344.
KEGGipae:PA4661.
PATRICi19844083. VBIPseAer58763_4883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08048.1.
PIRiF83062.
RefSeqiNP_253350.1. NC_002516.2.
WP_003099307.1. NZ_ASJY01000743.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ERVX-ray2.00A/B24-173[»]
ProteinModelPortaliQ9HVD1.
SMRiQ9HVD1. Positions 24-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4661.

Proteomic databases

PaxDbiQ9HVD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08048; AAG08048; PA4661.
GeneIDi881344.
KEGGipae:PA4661.
PATRICi19844083. VBIPseAer58763_4883.

Organism-specific databases

PseudoCAPiPA4661.

Phylogenomic databases

eggNOGiENOG41061N2. Bacteria.
ENOG4112AQW. LUCA.
HOGENOMiHOG000264842.
KOiK12976.
OMAiQPNDGVE.

Miscellaneous databases

EvolutionaryTraceiQ9HVD1.

Family and domain databases

Gene3Di2.40.160.20. 1 hit.
InterProiIPR018550. Lipid-A_deacylase-rel.
IPR011250. OMP/PagP_b-brl.
[Graphical view]
PfamiPF09411. PagL. 1 hit.
[Graphical view]
PIRSFiPIRSF029681. PagL. 1 hit.
SUPFAMiSSF56925. SSF56925. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAGL_PSEAE
AccessioniPrimary (citable) accession number: Q9HVD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.