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Protein

Lipid A deacylase PagL

Gene

pagL

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has lipid A 3-O-deacylase activity. Hydrolyzes the ester bond at the 3 position of lipid A, a bioactive component of lipopolysaccharide (LPS), thereby releasing the primary fatty acyl moiety. Lacks fatty acyl chain-length specificity as removes both 3-OH C10 and 3-OH C14 fatty acids from lipid A.3 Publications

Catalytic activityi

3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.3 Publications

Enzyme regulationi

Decreased activity at low temperatures (15 or 21 degrees Celsius).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149Charge relay system1 Publication1
Active sitei151Charge relay system1 Publication1
Sitei152Critical for activity1 Publication1
Active sitei163Charge relay system1 Publication1

GO - Molecular functioni

  • acyloxyacyl hydrolase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • lipid A metabolic process Source: UniProtKB
  • lipopolysaccharide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A deacylase PagL1 PublicationImported (EC:3.1.1.773 Publications)
Alternative name(s):
LPS 3-O-deacylase PagL1 Publication
Outer membrane enzyme PagL1 Publication
PhoP/PhoQ-activated gene product L1 Publication
Gene namesi
Name:pagLImported
Ordered Locus Names:PA4661
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4661.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 28Periplasmic1 Publication4
Transmembranei29 – 32Beta stranded1 Publication4
Topological domaini33Periplasmic1 Publication1
Transmembranei34 – 49Beta stranded1 PublicationAdd BLAST16
Topological domaini50 – 56Extracellular1 Publication7
Transmembranei57 – 71Beta stranded1 PublicationAdd BLAST15
Topological domaini72 – 73Periplasmic1 Publication2
Transmembranei74 – 89Beta stranded1 PublicationAdd BLAST16
Topological domaini90Extracellular1 Publication1
Transmembranei91 – 93Beta stranded1 Publication3
Topological domaini94 – 95Periplasmic1 Publication2
Transmembranei96 – 98Beta stranded1 Publication3
Topological domaini99 – 100Extracellular1 Publication2
Transmembranei101 – 115Beta stranded1 PublicationAdd BLAST15
Topological domaini116 – 117Periplasmic1 Publication2
Transmembranei118 – 128Beta stranded1 PublicationAdd BLAST11
Topological domaini129 – 138Extracellular1 Publication10
Transmembranei139 – 148Beta stranded1 Publication10
Topological domaini149 – 173Periplasmic1 PublicationAdd BLAST25

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Pharmaceutical usei

Might be useful for the development of new vaccines or adjuvants because of its LPS-modifying properties. May be used for the design of inhibitors with possible therapeutic value.1 Publication

Disruption phenotypei

Has similar growth characteristics to wild-type in LB medium at different temperatures (25, 30 or 37 degrees Celsius) or in LB medium supplemented with 0-4 M sodium chloride, 0-1,500 µg/ml chloramphenicol, 0-5% ethanol or 0-1% chloroform. Only when chloramphenicol is present in the medium is a difference in the ability to grow observed. The difference is most pronounced at a concentration of 650 µg/ml chloramphenicol, where the absorbance of the wild-type culture after overnight growth is 1.7-fold higher than that of the pagL disruption mutant.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81H → A or N: Does not affect LPS deacylation activity. 1 Publication1
Mutagenesisi84S → A or C: Does not affect LPS deacylation activity. 1 Publication1
Mutagenesisi149H → A or N: No deacylation of LPS. 1 Publication1
Mutagenesisi151S → A or C: No deacylation of LPS. 1 Publication1
Mutagenesisi152N → A: Inactive. 1 Publication1
Mutagenesisi159N → A: 304-fold reduction in activity. 1 Publication1
Mutagenesisi163E → A: 142-fold reduction in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysis1 PublicationAdd BLAST23
ChainiPRO_000042291224 – 173Lipid A deacylase PagLSequence analysis1 PublicationAdd BLAST150

Proteomic databases

PaxDbiQ9HVD1.
PRIDEiQ9HVD1.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

STRINGi208964.PA4661.

Structurei

Secondary structure

1173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi25 – 32Combined sources8
Beta strandi38 – 47Combined sources10
Beta strandi52 – 55Combined sources4
Beta strandi58 – 71Combined sources14
Beta strandi78 – 93Combined sources16
Beta strandi96 – 116Combined sources21
Beta strandi124 – 137Combined sources14
Beta strandi142 – 151Combined sources10
Beta strandi162 – 172Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ERVX-ray2.00A/B24-173[»]
ProteinModelPortaliQ9HVD1.
SMRiQ9HVD1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HVD1.

Family & Domainsi

Sequence similaritiesi

Belongs to the PagL family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG41061N2. Bacteria.
ENOG4112AQW. LUCA.
HOGENOMiHOG000264842.
KOiK12976.
OMAiQPNDGVE.

Family and domain databases

Gene3Di2.40.160.20. 1 hit.
InterProiIPR018550. Lipid-A_deacylase-rel.
IPR011250. OMP/PagP_b-brl.
[Graphical view]
PfamiPF09411. PagL. 1 hit.
[Graphical view]
PIRSFiPIRSF029681. PagL. 1 hit.
SUPFAMiSSF56925. SSF56925. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HVD1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLLPLAVL AALSSVHVAS AQAADVSAAV GATGQSGMTY RLGLSWDWDK
60 70 80 90 100
SWWQTSTGRL TGYWDAGYTY WEGGDEGAGK HSLSFAPVFV YEFAGDSIKP
110 120 130 140 150
FIEAGIGVAA FSGTRVGDQN LGSSLNFEDR IGAGLKFANG QSVGVRAIHY
160 170
SNAGLKQPND GIESYSLFYK IPI
Length:173
Mass (Da):18,394
Last modified:March 1, 2001 - v1
Checksum:i3B557C5787CD7B06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08048.1.
PIRiF83062.
RefSeqiNP_253350.1. NC_002516.2.
WP_003099307.1. NZ_ASJY01000743.1.

Genome annotation databases

EnsemblBacteriaiAAG08048; AAG08048; PA4661.
GeneIDi881344.
KEGGipae:PA4661.
PATRICi19844083. VBIPseAer58763_4883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08048.1.
PIRiF83062.
RefSeqiNP_253350.1. NC_002516.2.
WP_003099307.1. NZ_ASJY01000743.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ERVX-ray2.00A/B24-173[»]
ProteinModelPortaliQ9HVD1.
SMRiQ9HVD1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4661.

Proteomic databases

PaxDbiQ9HVD1.
PRIDEiQ9HVD1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08048; AAG08048; PA4661.
GeneIDi881344.
KEGGipae:PA4661.
PATRICi19844083. VBIPseAer58763_4883.

Organism-specific databases

PseudoCAPiPA4661.

Phylogenomic databases

eggNOGiENOG41061N2. Bacteria.
ENOG4112AQW. LUCA.
HOGENOMiHOG000264842.
KOiK12976.
OMAiQPNDGVE.

Miscellaneous databases

EvolutionaryTraceiQ9HVD1.

Family and domain databases

Gene3Di2.40.160.20. 1 hit.
InterProiIPR018550. Lipid-A_deacylase-rel.
IPR011250. OMP/PagP_b-brl.
[Graphical view]
PfamiPF09411. PagL. 1 hit.
[Graphical view]
PIRSFiPIRSF029681. PagL. 1 hit.
SUPFAMiSSF56925. SSF56925. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPAGL_PSEAE
AccessioniPrimary (citable) accession number: Q9HVD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2013
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Pharmaceutical, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.