Q9HVC5 (KPRS_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	PA4670

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP-Rule MF_00583
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00583
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP-Rule MF_00583
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00583.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	5-phosphoribose 1-diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: HAMAP ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Ribose-phosphate pyrophosphokinase HAMAP-Rule MF_00583		PRO_0000141176
Regions
	Region	212 – 225	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	129	1	Magnesium Potential
	Metal binding	131	1	Magnesium Potential
	Metal binding	140	1	Magnesium Potential
	Metal binding	144	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HVC5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E0868E996DEAD94C	FASTA	313	34,040
	10 20 30 40 50 60 MSKMMVFTGN ANPDLARRVV RQLHIPLGDV SVGKFSDGEI SVEINENVRG KDVFLIQPTC 70 80 90 100 110 120 APTNDNLMEL VVMADAFRRS SATRITAVIP YFGYARQDRR PRSARVAISA KVVADMLTVV 130 140 150 160 170 180 GVNRVLTVDL HADQIQGFFD IPVDNIYGSP VLVDDIEDQR FENLMIVSPD IGGVVRARAV 190 200 210 220 230 240 AKSLGVDLAI IDKRRPKANQ SEVMHIIGDV EGRTCVLVDD MVDTAGTLGH AAKALKEHGA 250 260 270 280 290 300 AKVIAYCTHP VLSGRAIENI EKSVLDELVV TNTIPLSAAA QACGRIRQLD IAPVVAEAMR 310 RISNEESISA MFR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08057.1.
PIR	G83063.
RefSeq	NP_253359.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HVC5.
SMR	Q9HVC5. Positions 4-308.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA4670.
Proteomic databases
PRIDE	Q9HVC5.
Protocols and materials databases
DNASU	881387.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881387.
KEGG	pae:PA4670.
PATRIC	19844103. VBIPseAer58763_4892.
Organism-specific databases
PseudoCAP	PA4670.
Phylogenomic databases
eggNOG	COG0462.
HOGENOM	HOG000210451.
KO	K00948.
OMA	YVQIQES.
ProtClustDB	PRK01259.
Enzyme and pathway databases
UniPathway	UPA00087; UER00172.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B.
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase_dom. IPR005946. Rib-P_diPkinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_PSEAE

Accession

Primary (citable) accession number: Q9HVC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program