Reviewed, UniProtKB/Swiss-Prot Q9HV66 (ACSA2_PSEAE)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase 2
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase 2
    Acyl-activating enzyme 2

Gene names

Name:	acsA2
Synonyms:	acsB
Ordered Locus Names:	PA4733

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Acetyl-coenzyme A synthetase 2 HAMAP MF_01123		PRO_0000208375
Sites
	Active site	514	1	By similarity
Amino acid modifications
	Modified residue	606	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HV66-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 03033C03913995D6	FASTA	645	71,624
	10 20 30 40 50 60 MFEISVHPVP DAVRQRAYLN DDDYQRLYRQ SVENPDEFWG EQAKAFLDWF KPWHSVHHGD 70 80 90 100 110 120 LRKGQATWFK GGQLNVAYNC IDRHLERRGE QIAIVWEGDN PSESAHITYR KLHHNVCRLA 130 140 150 160 170 180 NVLKSRGVEK GDRVCIYMPM IPEAAYAMLA CARIGAVHSV VFGGFSPDSL RDRILDADCR 190 200 210 220 230 240 TVITADEGVR GGKYIPLKQN VEKALKDCPD VSTVVVVERT QGDIPWVEGR DIWYHEALHA 250 260 270 280 290 300 ASADCPAEAM DAEDPLFILY TSGSTGKPKG VLHTTGGYLL GAAMTHKYVF DYHDGDVYWC 310 320 330 340 350 360 TADVGWVTGH SYIVYGPLAN GATTLMFEGV PNYPDASRFW QVIDKHQVNI FYTAPTAIRA 370 380 390 400 410 420 LMREGDAPVR QTSRSSLRLL GSVGEPINPE AWEWYYQVVG EKRCPILDTW WQTETGSILI 430 440 450 460 470 480 TPLPGATALK PGSATRPFFG VQPVLLDEKG KEIDGAGSGV LAIKASWPSQ IRSVYGDHQR 490 500 510 520 530 540 MIDTYFKPYP GYYFSGDGAR RDEDGYYWIT GRVDDVINVS GHRIGTAEVE SALVLHDAVA 550 560 570 580 590 600 EAAVVGCPHD VKGQAIYAFV TLMAGSQPSE ALQQELLALV GKEIGSFAKP DHLQWAPSLP 610 620 630 640 KTRSGKIMRR ILRKIACNEL DSLGDTSTLA DPGVVQGLID NRLNR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08119.1.
PIR	A83054.
RefSeq	NP_253421.1.
3D structure databases
SMR	Q9HV66. Positions 7-642.
ModBase	Search...
Genome annotation databases
GeneID	881650.
GenomeReviews	Gene locus PA4733 in contig AE004091_GR.
KEGG	pae:PA4733.
NMPDR	fig|208964.1.peg.4731.
Organism-specific databases
PseudoCAP	PA4733.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	HQRMVDT.
Enzyme and pathway databases
BioCyc	PAER208964:PA4733-MONOMER.
BRENDA	6.2.1.1. 354.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA2_PSEAE

Accession

Primary (citable) accession number: Q9HV66

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)