Q9HV37 (LLDD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-lactate dehydrogenase [cytochrome]
EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	PA4771

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity. HAMAP MF_01559
Subunit structure	Homotetramer By similarity. HAMAP MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lactate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206341
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HV37 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5BA544C711093559	FASTA	381	41,122
	10 20 30 40 50 60 MIISASTDYR AAAQRKLPPF LFHYIDGGAY AEYTLRRNVE DLSAIALRQR VLKNMSELSL 70 80 90 100 110 120 ETRLFDETLA MPVALAPVGL TGMYARRGEV QAARAAAAKG VPFTLSTVSV CPIEEVAPAI 130 140 150 160 170 180 DRPMWFQLYV LKDRGFMRNA LERAKAAGVT TLVFTVDMPV PGARYRDAHS GMSGPYAAPR 190 200 210 220 230 240 RILQAMTHPA WAWDVGLLGK PHDLGNISAY RGNPTGLEDY IGWLGANFDP SISWKDLEWI 250 260 270 280 290 300 REFWDGPMVI KGILDPEDAR DAVKFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGE 310 320 330 340 350 360 LAILADSGIR TGLDVVRMIA LGADSVLLGR AFVYALAAAG EAGVRNLLEL IEKEMRVAMV 370 380 LTGAKSIGEI SADSLVRELG A

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08157.1.
PIR	G83050.
RefSeq	NP_253459.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HV37.
ModBase	Search...
Proteomic databases
PRIDE	Q9HV37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881809.
GenomeReviews	Gene locus PA4771 in contig AE004091_GR.
KEGG	pae:PA4771.
PATRIC	19844331. VBIPseAer58763_4998.
Organism-specific databases
PseudoCAP	PA4771.
Phylogenomic databases
HOGENOM	HBG517781.
OMA	IGWLTEN.
ProtClustDB	PRK11197.
Enzyme and pathway databases
BioCyc	PAER208964:PA4771-MONOMER.
Family and domain databases
HAMAP	MF_01559. L-lact_dehydr. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012133. Alpha-hydoxy_acid_DH_FMN. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K00101.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_PSEAE

Accession

Primary (citable) accession number: Q9HV37

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program