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Q9HUV8 (PUR2_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:PA4855
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length429 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 429429Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151469

Regions

Domain108 – 315208ATP-grasp
Nucleotide binding134 – 19562ATP By similarity

Sites

Metal binding2851Magnesium or manganese By similarity
Metal binding2871Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HUV8 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 7DDCD05FD4A13441

FASTA42945,212
        10         20         30         40         50         60 
MNVLIIGSGG REHALAWKVA QDPRVAKVFV APGNAGTATE AKCENVAIDV LALEQLADFA 

        70         80         90        100        110        120 
AKNVQLTIVG PEAPLVAGVV DLFRERGLDI FGPTAGAAQL EGSKAFTKDF LARHRIPTAA 

       130        140        150        160        170        180 
YRNFTEVEPA LAYLHEQGAP IVIKADGLAA GKGVIVAMNL DEAEAAVRDM LAGNAFGDAG 

       190        200        210        220        230        240 
SRVVIEEFLD GEEASFIVMV DGQNVLPMAT SQDHKRVGDG DSGPNTGGMG AYSPAPVVTA 

       250        260        270        280        290        300 
EVHQRVLDEV IYPTVRGMAE EGNVYTGFLY AGLMIDKSGA PKVIEFNCRF GDPETQPIMV 

       310        320        330        340        350        360 
RLESSLVLLV EAALAKALDK VEATWDPRPT VGVVLAAGGY PGDYAKGEVI EGLAEAAALD 

       370        380        390        400        410        420 
GKVFHAGTAL KDGQVVTSGG RVLCATAIGE SVSAAQQQAY RLAEKIRWNG CFYRKDIGYR 


AIARERGES 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG08240.1.
PIRF83038.
RefSeqNP_253542.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9HUV8.
SMRQ9HUV8. Positions 1-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA4855.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID878236.
KEGGpae:PA4855.
PATRIC19844511. VBIPseAer58763_5087.

Organism-specific databases

PseudoCAPPA4855.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_PSEAE
AccessionPrimary (citable) accession number: Q9HUV8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways