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Protein

L-methionine sulfoximine/L-methionine sulfone acetyltransferase

Gene

pitA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the resistance against the toxic effects of L-methionine sulfoximine (MSX), a rare amino acid, which inhibits glutamine synthetase (GlnA). Catalyzes the acetylation of L-methionine sulfoximine (MSX).By similarity

Catalytic activityi

Acetyl-CoA + L-methionine sulfoximine = CoA + N-acetyl-L-methionine sulfoximine.By similarity
Acetyl-CoA + L-methionine sulfone = CoA + N-acetyl-L-methionine sulfone.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei127Acetyl-CoABy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.183. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
L-methionine sulfoximine/L-methionine sulfone acetyltransferaseBy similarity (EC:2.3.1.-By similarity)
Alternative name(s):
Methionine derivative detoxifier ABy similarity
Short name:
MDDABy similarity
Gene namesi
Name:pitA1 Publication
Ordered Locus Names:PA4866
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4866.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004333451 – 172L-methionine sulfoximine/L-methionine sulfone acetyltransferaseAdd BLAST172

Proteomic databases

PaxDbiQ9HUU7.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi208964.PA4866.

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Helixi10 – 12Combined sources3
Helixi13 – 26Combined sources14
Beta strandi28 – 31Combined sources4
Helixi38 – 51Combined sources14
Beta strandi55 – 59Combined sources5
Beta strandi65 – 77Combined sources13
Helixi78 – 80Combined sources3
Beta strandi83 – 90Combined sources8
Helixi92 – 94Combined sources3
Helixi99 – 113Combined sources15
Beta strandi118 – 124Combined sources7
Helixi128 – 136Combined sources9
Beta strandi140 – 151Combined sources12
Beta strandi154 – 165Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YVOX-ray1.90A/B1-172[»]
2BL1X-ray2.00A1-172[»]
ProteinModelPortaliQ9HUU7.
SMRiQ9HUU7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HUU7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 166N-acetyltransferasePROSITE-ProRule annotationAdd BLAST164

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni75 – 77Substrate bindingBy similarity3
Regioni85 – 87Substrate bindingBy similarity3
Regioni88 – 90Acetyl-CoA bindingBy similarity3
Regioni96 – 101Acetyl-CoA bindingBy similarity6

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiQ9HUU7.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiQ9HUU7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HUU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASIRDAGV ADLPGILAIY NDAVGNTTAI WNETPVDLAN RQAWFDTRAR
60 70 80 90 100
QGYPILVASD AAGEVLGYAS YGDWRPFEGF RGTVEHSVYV RDDQRGKGLG
110 120 130 140 150
VQLLQALIER ARAQGLHVMV AAIESGNAAS IGLHRRLGFE ISGQMPQVGQ
160 170
KFGRWLDLTF MQLNLDPTRS AP
Length:172
Mass (Da):18,743
Last modified:March 1, 2001 - v1
Checksum:i30E7117F5F15A826
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08251.1.
PIRiF83037.
RefSeqiNP_253553.1. NC_002516.2.
WP_003099390.1. NZ_ASJY01000773.1.

Genome annotation databases

EnsemblBacteriaiAAG08251; AAG08251; PA4866.
GeneIDi882184.
KEGGipae:PA4866.
PATRICi19844535. VBIPseAer58763_5099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08251.1.
PIRiF83037.
RefSeqiNP_253553.1. NC_002516.2.
WP_003099390.1. NZ_ASJY01000773.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YVOX-ray1.90A/B1-172[»]
2BL1X-ray2.00A1-172[»]
ProteinModelPortaliQ9HUU7.
SMRiQ9HUU7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4866.

Proteomic databases

PaxDbiQ9HUU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08251; AAG08251; PA4866.
GeneIDi882184.
KEGGipae:PA4866.
PATRICi19844535. VBIPseAer58763_5099.

Organism-specific databases

PseudoCAPiPA4866.

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiQ9HUU7.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiQ9HUU7.

Enzyme and pathway databases

BRENDAi2.3.1.183. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HUU7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDDA_PSEAE
AccessioniPrimary (citable) accession number: Q9HUU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.