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Protein

L-methionine sulfoximine/L-methionine sulfone acetyltransferase

Gene

pitA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the resistance against the toxic effects of L-methionine sulfoximine (MSX), a rare amino acid, which inhibits glutamine synthetase (GlnA). Catalyzes the acetylation of L-methionine sulfoximine (MSX).By similarity

Catalytic activityi

Acetyl-CoA + L-methionine sulfoximine = CoA + N-acetyl-L-methionine sulfoximine.By similarity
Acetyl-CoA + L-methionine sulfone = CoA + N-acetyl-L-methionine sulfone.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271Acetyl-CoABy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.183. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
L-methionine sulfoximine/L-methionine sulfone acetyltransferaseBy similarity (EC:2.3.1.-By similarity)
Alternative name(s):
Methionine derivative detoxifier ABy similarity
Short name:
MDDABy similarity
Gene namesi
Name:pitA1 Publication
Ordered Locus Names:PA4866
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4866.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 172172L-methionine sulfoximine/L-methionine sulfone acetyltransferasePRO_0000433345Add
BLAST

Proteomic databases

PaxDbiQ9HUU7.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi208964.PA4866.

Structurei

Secondary structure

1
172
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Helixi10 – 123Combined sources
Helixi13 – 2614Combined sources
Beta strandi28 – 314Combined sources
Helixi38 – 5114Combined sources
Beta strandi55 – 595Combined sources
Beta strandi65 – 7713Combined sources
Helixi78 – 803Combined sources
Beta strandi83 – 908Combined sources
Helixi92 – 943Combined sources
Helixi99 – 11315Combined sources
Beta strandi118 – 1247Combined sources
Helixi128 – 1369Combined sources
Beta strandi140 – 15112Combined sources
Beta strandi154 – 16512Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVOX-ray1.90A/B1-172[»]
2BL1X-ray2.00A1-172[»]
ProteinModelPortaliQ9HUU7.
SMRiQ9HUU7. Positions 2-172.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HUU7.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 166164N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 773Substrate bindingBy similarity
Regioni85 – 873Substrate bindingBy similarity
Regioni88 – 903Acetyl-CoA bindingBy similarity
Regioni96 – 1016Acetyl-CoA bindingBy similarity

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiQ9HUU7.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiQ9HUU7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HUU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASIRDAGV ADLPGILAIY NDAVGNTTAI WNETPVDLAN RQAWFDTRAR
60 70 80 90 100
QGYPILVASD AAGEVLGYAS YGDWRPFEGF RGTVEHSVYV RDDQRGKGLG
110 120 130 140 150
VQLLQALIER ARAQGLHVMV AAIESGNAAS IGLHRRLGFE ISGQMPQVGQ
160 170
KFGRWLDLTF MQLNLDPTRS AP
Length:172
Mass (Da):18,743
Last modified:March 1, 2001 - v1
Checksum:i30E7117F5F15A826
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08251.1.
PIRiF83037.
RefSeqiNP_253553.1. NC_002516.2.
WP_003099390.1. NZ_ASJY01000773.1.

Genome annotation databases

EnsemblBacteriaiAAG08251; AAG08251; PA4866.
GeneIDi882184.
KEGGipae:PA4866.
PATRICi19844535. VBIPseAer58763_5099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08251.1.
PIRiF83037.
RefSeqiNP_253553.1. NC_002516.2.
WP_003099390.1. NZ_ASJY01000773.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YVOX-ray1.90A/B1-172[»]
2BL1X-ray2.00A1-172[»]
ProteinModelPortaliQ9HUU7.
SMRiQ9HUU7. Positions 2-172.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4866.

Proteomic databases

PaxDbiQ9HUU7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08251; AAG08251; PA4866.
GeneIDi882184.
KEGGipae:PA4866.
PATRICi19844535. VBIPseAer58763_5099.

Organism-specific databases

PseudoCAPiPA4866.

Phylogenomic databases

eggNOGiENOG4108Z7F. Bacteria.
COG1247. LUCA.
HOGENOMiHOG000078516.
InParanoidiQ9HUU7.
KOiK03823.
OMAiVTGQMPQ.
PhylomeDBiQ9HUU7.

Enzyme and pathway databases

BRENDAi2.3.1.183. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HUU7.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDDA_PSEAE
AccessioniPrimary (citable) accession number: Q9HUU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.