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Protein

Urease subunit alpha

Gene

ureC

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi133 – 1331Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi135 – 1351Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi216 – 2161Nickel 1; via carbamate groupUniRule annotation
Metal bindingi216 – 2161Nickel 2; via carbamate groupUniRule annotation
Binding sitei218 – 2181SubstrateUniRule annotation
Metal bindingi245 – 2451Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi271 – 2711Nickel 2; via tele nitrogenUniRule annotation
Active sitei319 – 3191Proton donorUniRule annotation
Metal bindingi359 – 3591Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:PA4868
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4868.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 566566Urease subunit alphaPRO_0000234167Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei216 – 2161N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Proteomic databases

PaxDbiQ9HUU5.

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA4868.

Structurei

3D structure databases

ProteinModelPortaliQ9HUU5.
SMRiQ9HUU5. Positions 3-566.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini128 – 566439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
InParanoidiQ9HUU5.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.
PhylomeDBiQ9HUU5.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HUU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISRQAYAD MFGPTVGDRV RLADTDLWIE VERDFTVYGE EVKFGGGKVI
60 70 80 90 100
RDGMGQSQLG AAQVVDTVIT NALILDHWGV VKADVGLKDG RIQAIGKAGN
110 120 130 140 150
PDIQPGVNIA IGAGTEVIAG EGMILTAGGI DTHIHFICPQ QIEEALMSGV
160 170 180 190 200
TTMIGGGTGP AAGTNATTCT SGPWHMARML QAADAFPMNI GFTGKGNASL
210 220 230 240 250
PLPLEEQVLA GAIGLKLHED WGSTPAAIDN CLEVAERHDI QVAIHTDTLN
260 270 280 290 300
ESGFVETTLG AFKGRTIHTY HTEGAGGGHA PDIIKACGFA NVLPSSTNPT
310 320 330 340 350
RPFTRNTIDE HLDMLMVCHH LDPAIAEDVA FAESRIRRET IAAEDILHDL
360 370 380 390 400
GAFSMISSDS QAMGRVGEVI TRTWQTADKM KRQRGRLDGD GARNDNFRAR
410 420 430 440 450
RYIAKYTINP AITHGISHEV GSVEAGKWAD LVLWRPAFFG VKPSLILKGG
460 470 480 490 500
AIAASLMGDI NGSIPTPQPV HYRPMFASYA GSRHATSLTF VSQAAFAAGV
510 520 530 540 550
PQQLGLRKAI GVVSGCRGVQ KTDLIHNGYL PTIEVDAQNY QVRADGQLLW
560
CEPADVLPMA QRYFLF
Length:566
Mass (Da):60,611
Last modified:March 1, 2001 - v1
Checksum:i5A3C167356DFB3AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08253.1.
PIRiH83037.
RefSeqiNP_253555.1. NC_002516.2.
WP_003099392.1. NZ_ASJY01000773.1.

Genome annotation databases

EnsemblBacteriaiAAG08253; AAG08253; PA4868.
GeneIDi878240.
KEGGipae:PA4868.
PATRICi19844539. VBIPseAer58763_5101.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08253.1.
PIRiH83037.
RefSeqiNP_253555.1. NC_002516.2.
WP_003099392.1. NZ_ASJY01000773.1.

3D structure databases

ProteinModelPortaliQ9HUU5.
SMRiQ9HUU5. Positions 3-566.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4868.

Protein family/group databases

MEROPSiM38.982.

Proteomic databases

PaxDbiQ9HUU5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08253; AAG08253; PA4868.
GeneIDi878240.
KEGGipae:PA4868.
PATRICi19844539. VBIPseAer58763_5101.

Organism-specific databases

PseudoCAPiPA4868.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
InParanoidiQ9HUU5.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.
PhylomeDBiQ9HUU5.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiURE1_PSEAE
AccessioniPrimary (citable) accession number: Q9HUU5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.