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Protein

Alanine racemase, biosynthetic

Gene

alr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

Kineticsi

  1. KM=1.1 mM for D-alanine1 Publication
  2. KM=1.1 mM for L-alanine1 Publication
  1. Vmax=40 µmol/min/mg enzyme toward D-alanine1 Publication
  2. Vmax=44 µmol/min/mg enzyme toward L-alanine1 Publication

Pathwayi: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase, biosynthetic (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34Proton acceptor; specific for D-alanineBy similarity1
Binding sitei130SubstrateBy similarity1
Active sitei254Proton acceptor; specific for L-alanineBy similarity1
Binding sitei302Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
UPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, biosynthetic (EC:5.1.1.1)
Gene namesi
Name:alr
Ordered Locus Names:PA4930
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4930.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145461 – 358Alanine racemase, biosyntheticAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9HUN4.
PRIDEiQ9HUN4.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4930.

Structurei

3D structure databases

ProteinModelPortaliQ9HUN4.
SMRiQ9HUN4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HUN4.
KOiK01775.
OMAiDTGFNRL.
PhylomeDBiQ9HUN4.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HUN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLVATVDL SAIRHNYALA KRCAPQRQAF AVVKANAYGH GAREVVTALH
60 70 80 90 100
DDADGFAVAC LEEAAEVRAL HASARILLLE GCFEASEYAL AGQLRLDLVI
110 120 130 140 150
QGAEQGEAFL AAGLDIPLNV WLKLDSGMHR LGFDPAALRA WHARLRSHPG
160 170 180 190 200
VRELNLISHF ACADERNHPL TEQQLESFLG LLDLDFDQRS LANSAAVLTI
210 220 230 240 250
PAAHMDWLRP GIMLYGSTPL ADLSAAELGL KPAMSLGAQL ISLREVAVGE
260 270 280 290 300
SVGYGATWIA ERPARIGTVS CGYADGYPRT APAGTPVLVG GRRAILAGRV
310 320 330 340 350
SMDMLAVDLS DLPEARVGDP VELWGAGLSV DEVARACGTL GYELLSKVTA

RVPRRYSH
Length:358
Mass (Da):38,311
Last modified:March 1, 2001 - v1
Checksum:iBAA99415A8948BD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147S → N in AAD47082 (PubMed:10977898).Curated1
Sequence conflicti295I → L in AAD47082 (PubMed:10977898).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165882 Genomic DNA. Translation: AAD47082.1.
AE004091 Genomic DNA. Translation: AAG08315.1.
PIRiD83029.
RefSeqiNP_253617.1. NC_002516.2.
WP_003112284.1. NZ_ASJY01000788.1.

Genome annotation databases

EnsemblBacteriaiAAG08315; AAG08315; PA4930.
GeneIDi878646.
KEGGipae:PA4930.
PATRICi19844664. VBIPseAer58763_5163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165882 Genomic DNA. Translation: AAD47082.1.
AE004091 Genomic DNA. Translation: AAG08315.1.
PIRiD83029.
RefSeqiNP_253617.1. NC_002516.2.
WP_003112284.1. NZ_ASJY01000788.1.

3D structure databases

ProteinModelPortaliQ9HUN4.
SMRiQ9HUN4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4930.

Proteomic databases

PaxDbiQ9HUN4.
PRIDEiQ9HUN4.

Protocols and materials databases

DNASUi878646.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08315; AAG08315; PA4930.
GeneIDi878646.
KEGGipae:PA4930.
PATRICi19844664. VBIPseAer58763_5163.

Organism-specific databases

PseudoCAPiPA4930.

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HUN4.
KOiK01775.
OMAiDTGFNRL.
PhylomeDBiQ9HUN4.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
UPA00219.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALR1_PSEAE
AccessioniPrimary (citable) accession number: Q9HUN4
Secondary accession number(s): Q9S418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.