Q9HUN4 (ALR1_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase, biosynthetic
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	PA4930

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Alanine racemase, biosynthetic HAMAP MF_01201		PRO_0000114546
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	254	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	147	1	S → N in AAD47082. Ref.1
	Sequence conflict	295	1	I → L in AAD47082. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9HUN4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BAA99415A8948BD6	FASTA	358	38,311
	10 20 30 40 50 60 MRPLVATVDL SAIRHNYALA KRCAPQRQAF AVVKANAYGH GAREVVTALH DDADGFAVAC 70 80 90 100 110 120 LEEAAEVRAL HASARILLLE GCFEASEYAL AGQLRLDLVI QGAEQGEAFL AAGLDIPLNV 130 140 150 160 170 180 WLKLDSGMHR LGFDPAALRA WHARLRSHPG VRELNLISHF ACADERNHPL TEQQLESFLG 190 200 210 220 230 240 LLDLDFDQRS LANSAAVLTI PAAHMDWLRP GIMLYGSTPL ADLSAAELGL KPAMSLGAQL 250 260 270 280 290 300 ISLREVAVGE SVGYGATWIA ERPARIGTVS CGYADGYPRT APAGTPVLVG GRRAILAGRV 310 320 330 340 350 SMDMLAVDLS DLPEARVGDP VELWGAGLSV DEVARACGTL GYELLSKVTA RVPRRYSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1." Strych U., Huang H.-C., Krause K.L., Benedik M.J. Curr. Microbiol. 41:290-294(2000) [PubMed: 10977898] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF165882 Genomic DNA. Translation: AAD47082.1. AE004091 Genomic DNA. Translation: AAG08315.1.
PIR	D83029.
RefSeq	NP_253617.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9HUN4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878646.
GenomeReviews	Gene locus PA4930 in contig AE004091_GR.
KEGG	pae:PA4930.
NMPDR	fig|208964.1.peg.4927.
PATRIC	19844664. VBIPseAer58763_5163.
Organism-specific databases
PseudoCAP	PA4930.
Phylogenomic databases
HOGENOM	HBG712172.
OMA	HYEIACN.
ProtClustDB	CLSK869000.
Enzyme and pathway databases
BioCyc	PAER208964:PA4930-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR1_PSEAE

Accession

Primary (citable) accession number: Q9HUN4
Secondary accession number(s): Q9S418

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program