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Protein

Alanine racemase, biosynthetic

Gene

alr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

Kineticsi

  1. KM=1.1 mM for D-alanine1 Publication
  2. KM=1.1 mM for L-alanine1 Publication
  1. Vmax=40 µmol/min/mg enzyme toward D-alanine1 Publication
  2. Vmax=44 µmol/min/mg enzyme toward L-alanine1 Publication

Pathwayi: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase, biosynthetic (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei34Proton acceptor; specific for D-alanineBy similarity1
Binding sitei130SubstrateBy similarity1
Active sitei254Proton acceptor; specific for L-alanineBy similarity1
Binding sitei302Substrate; via amide nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-5044-MONOMER
SABIO-RKiQ9HUN4
UniPathwayiUPA00042; UER00497
UPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, biosynthetic (EC:5.1.1.1)
Gene namesi
Name:alr
Ordered Locus Names:PA4930
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4930

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145461 – 358Alanine racemase, biosyntheticAdd BLAST358

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei34N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ9HUN4
PRIDEiQ9HUN4

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4930

Structurei

3D structure databases

ProteinModelPortaliQ9HUN4
SMRiQ9HUN4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJ4 Bacteria
COG0787 LUCA
HOGENOMiHOG000031446
InParanoidiQ9HUN4
KOiK01775
OMAiISHFACA
PhylomeDBiQ9HUN4

Family and domain databases

Gene3Di2.40.37.10, 2 hits
3.20.20.10, 1 hit
HAMAPiMF_01201 Ala_racemase, 1 hit
InterProiView protein in InterPro
IPR000821 Ala_racemase
IPR009006 Ala_racemase/Decarboxylase_C
IPR011079 Ala_racemase_C
IPR001608 Ala_racemase_N
IPR020622 Ala_racemase_pyridoxalP-BS
IPR029066 PLP-binding_barrel
PfamiView protein in Pfam
PF00842 Ala_racemase_C, 1 hit
PF01168 Ala_racemase_N, 1 hit
PRINTSiPR00992 ALARACEMASE
SMARTiView protein in SMART
SM01005 Ala_racemase_C, 1 hit
SUPFAMiSSF50621 SSF50621, 1 hit
SSF51419 SSF51419, 1 hit
TIGRFAMsiTIGR00492 alr, 1 hit
PROSITEiView protein in PROSITE
PS00395 ALANINE_RACEMASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HUN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLVATVDL SAIRHNYALA KRCAPQRQAF AVVKANAYGH GAREVVTALH
60 70 80 90 100
DDADGFAVAC LEEAAEVRAL HASARILLLE GCFEASEYAL AGQLRLDLVI
110 120 130 140 150
QGAEQGEAFL AAGLDIPLNV WLKLDSGMHR LGFDPAALRA WHARLRSHPG
160 170 180 190 200
VRELNLISHF ACADERNHPL TEQQLESFLG LLDLDFDQRS LANSAAVLTI
210 220 230 240 250
PAAHMDWLRP GIMLYGSTPL ADLSAAELGL KPAMSLGAQL ISLREVAVGE
260 270 280 290 300
SVGYGATWIA ERPARIGTVS CGYADGYPRT APAGTPVLVG GRRAILAGRV
310 320 330 340 350
SMDMLAVDLS DLPEARVGDP VELWGAGLSV DEVARACGTL GYELLSKVTA

RVPRRYSH
Length:358
Mass (Da):38,311
Last modified:March 1, 2001 - v1
Checksum:iBAA99415A8948BD6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti147S → N in AAD47082 (PubMed:10977898).Curated1
Sequence conflicti295I → L in AAD47082 (PubMed:10977898).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165882 Genomic DNA Translation: AAD47082.1
AE004091 Genomic DNA Translation: AAG08315.1
PIRiD83029
RefSeqiNP_253617.1, NC_002516.2
WP_003112284.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG08315; AAG08315; PA4930
GeneIDi878646
KEGGipae:PA4930
PATRICifig|208964.12.peg.5163

Similar proteinsi

Entry informationi

Entry nameiALR1_PSEAE
AccessioniPrimary (citable) accession number: Q9HUN4
Secondary accession number(s): Q9S418
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health