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Protein

Alanine racemase, biosynthetic

Gene

alr

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

Kineticsi

  1. KM=1.1 mM for D-alanine1 Publication
  2. KM=1.1 mM for L-alanine1 Publication
  1. Vmax=40 µmol/min/mg enzyme toward D-alanine1 Publication
  2. Vmax=44 µmol/min/mg enzyme toward L-alanine1 Publication

Pathwayi: D-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase, biosynthetic (alr)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Proton acceptor; specific for D-alanineBy similarity
Binding sitei130 – 1301SubstrateBy similarity
Active sitei254 – 2541Proton acceptor; specific for L-alanineBy similarity
Binding sitei302 – 3021Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
UPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, biosynthetic (EC:5.1.1.1)
Gene namesi
Name:alr
Ordered Locus Names:PA4930
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4930.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Alanine racemase, biosyntheticPRO_0000114546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PaxDbiQ9HUN4.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA4930.

Structurei

3D structure databases

ProteinModelPortaliQ9HUN4.
SMRiQ9HUN4. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.Curated

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HUN4.
KOiK01775.
OMAiIVHMTHF.
OrthoDBiEOG6PP9NJ.
PhylomeDBiQ9HUN4.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HUN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPLVATVDL SAIRHNYALA KRCAPQRQAF AVVKANAYGH GAREVVTALH
60 70 80 90 100
DDADGFAVAC LEEAAEVRAL HASARILLLE GCFEASEYAL AGQLRLDLVI
110 120 130 140 150
QGAEQGEAFL AAGLDIPLNV WLKLDSGMHR LGFDPAALRA WHARLRSHPG
160 170 180 190 200
VRELNLISHF ACADERNHPL TEQQLESFLG LLDLDFDQRS LANSAAVLTI
210 220 230 240 250
PAAHMDWLRP GIMLYGSTPL ADLSAAELGL KPAMSLGAQL ISLREVAVGE
260 270 280 290 300
SVGYGATWIA ERPARIGTVS CGYADGYPRT APAGTPVLVG GRRAILAGRV
310 320 330 340 350
SMDMLAVDLS DLPEARVGDP VELWGAGLSV DEVARACGTL GYELLSKVTA

RVPRRYSH
Length:358
Mass (Da):38,311
Last modified:March 1, 2001 - v1
Checksum:iBAA99415A8948BD6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti147 – 1471S → N in AAD47082 (PubMed:10977898).Curated
Sequence conflicti295 – 2951I → L in AAD47082 (PubMed:10977898).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165882 Genomic DNA. Translation: AAD47082.1.
AE004091 Genomic DNA. Translation: AAG08315.1.
PIRiD83029.
RefSeqiNP_253617.1. NC_002516.2.
WP_003112284.1. NZ_ASJY01000788.1.

Genome annotation databases

EnsemblBacteriaiAAG08315; AAG08315; PA4930.
GeneIDi878646.
KEGGipae:PA4930.
PATRICi19844664. VBIPseAer58763_5163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165882 Genomic DNA. Translation: AAD47082.1.
AE004091 Genomic DNA. Translation: AAG08315.1.
PIRiD83029.
RefSeqiNP_253617.1. NC_002516.2.
WP_003112284.1. NZ_ASJY01000788.1.

3D structure databases

ProteinModelPortaliQ9HUN4.
SMRiQ9HUN4. Positions 1-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4930.

Proteomic databases

PaxDbiQ9HUN4.

Protocols and materials databases

DNASUi878646.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08315; AAG08315; PA4930.
GeneIDi878646.
KEGGipae:PA4930.
PATRICi19844664. VBIPseAer58763_5163.

Organism-specific databases

PseudoCAPiPA4930.

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HUN4.
KOiK01775.
OMAiIVHMTHF.
OrthoDBiEOG6PP9NJ.
PhylomeDBiQ9HUN4.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
UPA00219.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1."
    Strych U., Huang H.-C., Krause K.L., Benedik M.J.
    Curr. Microbiol. 41:290-294(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiALR1_PSEAE
AccessioniPrimary (citable) accession number: Q9HUN4
Secondary accession number(s): Q9S418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.