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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.

Cofactori

Mg2+By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 19ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferase (EC:2.5.1.75)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name:
DMAPP:tRNA dimethylallyltransferase
Short name:
DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name:
IPP transferase
Short name:
IPPT
Short name:
IPTase
Gene namesi
Name:miaA
Ordered Locus Names:PA4945
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4945.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001639561 – 323tRNA dimethylallyltransferaseAdd BLAST323

Proteomic databases

PaxDbiQ9HUL9.

Interactioni

Subunit structurei

Monomer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei103Interaction with substrate tRNABy similarity1
Sitei125Interaction with substrate tRNABy similarity1

Protein-protein interaction databases

STRINGi208964.PA4945.

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 11Combined sources6
Helixi18 – 28Combined sources11
Beta strandi31 – 36Combined sources6
Turni38 – 41Combined sources4
Turni47 – 50Combined sources4
Helixi54 – 59Combined sources6
Beta strandi62 – 66Combined sources5
Helixi76 – 92Combined sources17
Beta strandi96 – 102Combined sources7
Helixi104 – 111Combined sources8
Beta strandi201 – 209Combined sources9
Helixi213 – 229Combined sources17
Helixi232 – 240Combined sources9
Helixi251 – 253Combined sources3
Helixi257 – 264Combined sources8
Helixi270 – 293Combined sources24
Beta strandi299 – 302Combined sources4
Helixi308 – 318Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CRMX-ray1.90A1-323[»]
3CRQX-ray2.20A1-323[»]
3CRRX-ray1.90A1-323[»]
ProteinModelPortaliQ9HUL9.
SMRiQ9HUL9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HUL9.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 19Substrate bindingBy similarity6
Regioni37 – 40Interaction with substrate tRNABy similarity4
Regioni161 – 165Interaction with substrate tRNABy similarity5
Regioni253 – 258Interaction with substrate tRNABy similarity6
Regioni286 – 293Interaction with substrate tRNABy similarity8

Sequence similaritiesi

Belongs to the IPP transferase family.Curated

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
InParanoidiQ9HUL9.
KOiK00791.
OMAiHTRNYAK.
PhylomeDBiQ9HUL9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HUL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLPPAIFL MGPTAAGKTD LAMALADALP CELISVDSAL IYRGMDIGTA
60 70 80 90 100
KPSRELLARY PHRLIDIRDP AESYSAAEFR ADALAAMAKA TARGRIPLLV
110 120 130 140 150
GGTMLYYKAL LEGLADMPGA DPEVRAAIEA EAQAEGWEAL HRQLAEVDPE
160 170 180 190 200
SAARIHPNDP QRLMRALEVY RLGGVSMSDL RRRQSAEKAD FDASGRNQLP
210 220 230 240 250
YTVAQLAIAP EQRQVLHARI AQRFRQMLEQ GFIAEVEALH ARSDLHAGLP
260 270 280 290 300
SIRAVGYRQV WDYLDGKLSY AEMTERGIIA TRQLAKRQFT WLRSWSHLHW
310 320
MDSLAGDNLP RALKYLKTVS ILA
Length:323
Mass (Da):35,769
Last modified:March 1, 2001 - v1
Checksum:iB33DFB9EA45157CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08330.1.
PIRiE83028.
RefSeqiNP_253632.1. NC_002516.2.
WP_003113929.1. NZ_ASJY01000789.1.

Genome annotation databases

EnsemblBacteriaiAAG08330; AAG08330; PA4945.
GeneIDi878016.
KEGGipae:PA4945.
PATRICi19844698. VBIPseAer58763_5178.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08330.1.
PIRiE83028.
RefSeqiNP_253632.1. NC_002516.2.
WP_003113929.1. NZ_ASJY01000789.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CRMX-ray1.90A1-323[»]
3CRQX-ray2.20A1-323[»]
3CRRX-ray1.90A1-323[»]
ProteinModelPortaliQ9HUL9.
SMRiQ9HUL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA4945.

Proteomic databases

PaxDbiQ9HUL9.

Protocols and materials databases

DNASUi878016.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08330; AAG08330; PA4945.
GeneIDi878016.
KEGGipae:PA4945.
PATRICi19844698. VBIPseAer58763_5178.

Organism-specific databases

PseudoCAPiPA4945.

Phylogenomic databases

eggNOGiENOG4105DKX. Bacteria.
COG0324. LUCA.
HOGENOMiHOG000039996.
InParanoidiQ9HUL9.
KOiK00791.
OMAiHTRNYAK.
PhylomeDBiQ9HUL9.

Miscellaneous databases

EvolutionaryTraceiQ9HUL9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_PSEAE
AccessioniPrimary (citable) accession number: Q9HUL9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.