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Protein

Lipid A export ATP-binding/permease protein MsbA

Gene

msbA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi390 – 397ATPUniRule annotation8

GO - Molecular functioni

  • ATPase activity Source: PseudoCAP
  • ATP binding Source: UniProtKB-KW
  • ATP transmembrane transporter activity Source: PseudoCAP
  • lipid-transporting ATPase activity Source: GO_Central

GO - Biological processi

  • lipid A biosynthetic process Source: PseudoCAP
  • lipopolysaccharide transport Source: PseudoCAP

Keywordsi

Molecular functionHydrolase
Biological processLipid transport, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.39. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A export ATP-binding/permease protein MsbAUniRule annotation (EC:3.6.3.-UniRule annotation)
Gene namesi
Name:msbAUniRule annotation
Ordered Locus Names:PA4997
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA4997.

Subcellular locationi

Q9HUG8:
  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei79 – 99HelicalUniRule annotationAdd BLAST21
Transmembranei161 – 178HelicalUniRule annotationAdd BLAST18
Transmembranei183 – 202HelicalUniRule annotationAdd BLAST20
Transmembranei269 – 289HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000925931 – 603Lipid A export ATP-binding/permease protein MsbAAdd BLAST603

Proteomic databases

PaxDbiQ9HUG8.
PRIDEiQ9HUG8.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA4997.

Structurei

3D structure databases

ProteinModelPortaliQ9HUG8.
SMRiQ9HUG8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 324ABC transmembrane type-1UniRule annotationAdd BLAST295
Domaini356 – 592ABC transporterUniRule annotationAdd BLAST237

Domaini

In MsbA the ATP-binding domain (NBD) and the transmembrane domain (TMD) are fused.

Sequence similaritiesi

Belongs to the ABC transporter superfamily. Lipid exporter (TC 3.A.1.106) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ1. Bacteria.
COG1132. LUCA.
InParanoidiQ9HUG8.
KOiK11085.
OMAiSPLMEFL.
PhylomeDBiQ9HUG8.

Family and domain databases

Gene3Di1.20.1560.10. 1 hit.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR011917. ABC_transpr_lipidA.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
TIGRFAMsiTIGR02203. MsbA_lipidA. 1 hit.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51239. MSBA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HUG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDSPQNPGP SSLKIYFRLL GYVKPYIGMF LLSIVGFLIF ASTQPMLAGI
60 70 80 90 100
LKYFVDGLSN PDAALFPNVQ WPWLRDLHLV YAVPLLIILI AAWQGLGSFL
110 120 130 140 150
GNFFLAKVSL GLVHDLRVAL FNKLLVLPNR YFDTHSSGHL ISRITFNVTM
160 170 180 190 200
VTGAATDAIK VVIREGLTVV FLFLYLLWMN WKLTLVMLAI LPVIAVMVTT
210 220 230 240 250
ASRKFRKQSK KIQVAMGDVT HVASETIQGY RVVRSFGGEA YEEKRFLDAS
260 270 280 290 300
QSNTDKQLRM TKTGAVYTPM LQLVIYVAMA ILMFLVLWLR GDASAGDLVA
310 320 330 340 350
YITAAGLLPK PIRQLSEVSS TVQRGVAGAE SIFEQLDEAA EEDQGTVEKE
360 370 380 390 400
RVSGRLEVRN LSFRYPGTDK QVLDDISFIA EPGQMIALVG RSGSGKSTLA
410 420 430 440 450
NLVPRFYQHN DGKILLDGVE VEDYRLRNLR RHIALVTQQV TLFNDSVANN
460 470 480 490 500
IAYGDLAGAP REEIERAAKA ANAKEFIDNL PQGFDTEVGE NGVLLSGGQR
510 520 530 540 550
QRLAIARALL KDAPLLILDE ATSALDTESE RHIQAALDEV MKGRTTLVIA
560 570 580 590 600
HRLSTIEKAD LILVMDQGQI VERGSHAELL AQNGHYARLH AMGLDEQAPA

PVG
Length:603
Mass (Da):66,432
Last modified:March 1, 2001 - v1
Checksum:i4A91DA2845BCF5C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08382.1.
PIRiB83022.
RefSeqiNP_253684.1. NC_002516.2.
WP_003114544.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG08382; AAG08382; PA4997.
GeneIDi881672.
KEGGipae:PA4997.
PATRICifig|208964.12.peg.5237.

Similar proteinsi

Entry informationi

Entry nameiMSBA_PSEAE
AccessioniPrimary (citable) accession number: Q9HUG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2001
Last modified: October 25, 2017
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families