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Q9HUF7

- RFAP_PSEAE

UniProt

Q9HUF7 - RFAP_PSEAE

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Protein

Lipopolysaccharide core heptose(I) kinase RfaP

Gene

rfaP

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of heptose(I) of the outer membrane lipopolysaccharide core. The phosphorylation of the lipopolysaccharide core seems to occur prior to translocation to the periplasm and attachment of O-antigen. Also has protein-tyrosine kinase activity: autophosphorylates on all Tyr residues; in vitro can phosphorylate poly(Glu,Tyr).1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Cofactori

Magnesium.By similarity

Kineticsi

For lipopolysaccharide kinase activity.

  1. KM=0.22 mM for ATP1 Publication
  2. KM=14.4 µM for hydrofluoric acid-treated lipopolysaccharide1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei163 – 1631

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: PseudoCAP
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. lipopolysaccharide core region biosynthetic process Source: PseudoCAP
  2. response to drug Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00958.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide core heptose(I) kinase RfaP (EC:2.7.1.-, EC:2.7.10.2)
Alternative name(s):
Lipopolysaccharide kinase WaaP
Gene namesi
Name:rfaP
Synonyms:waaP
Ordered Locus Names:PA5009
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA5009.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Lack of viability.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi69 – 691K → A or R: Loss of protein-tyrosine kinase activity. 1 Publication
Mutagenesisi163 – 1631D → A or E: Loss of protein-tyrosine kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 268268Lipopolysaccharide core heptose(I) kinase RfaPPRO_0000395801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphotyrosine; by autocatalysis1 Publication
Modified residuei48 – 481Phosphotyrosine; by autocatalysis1 Publication
Modified residuei98 – 981Phosphotyrosine; by autocatalysis1 Publication
Modified residuei165 – 1651Phosphotyrosine; by autocatalysis1 Publication
Modified residuei211 – 2111Phosphotyrosine; by autocatalysis1 Publication
Modified residuei231 – 2311Phosphotyrosine; by autocatalysis1 Publication
Modified residuei258 – 2581Phosphotyrosine; by autocatalysis1 Publication
Modified residuei264 – 2641Phosphotyrosine; by autocatalysis1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5009.

Structurei

3D structure databases

ProteinModelPortaliQ9HUF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG04355.
HOGENOMiHOG000126215.
InParanoidiQ9HUF7.
KOiK02848.
OMAiLGAGQEW.
OrthoDBiEOG63JR6W.
PhylomeDBiQ9HUF7.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR017172. Lsacc_core_hep_kinase_RfaP.
[Graphical view]
PfamiPF06293. Kdo. 1 hit.
[Graphical view]
PIRSFiPIRSF037318. RfaP. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HUF7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLVLEEPFK RLWNGRDPFE AVEALQGKVY RELEGRRTLR TEVDGRGYFV
60 70 80 90 100
KIHRGIGWGE IAKNLLTAKL PVLGARQEWQ AIRRLHEAGV ATMTAVAYGE
110 120 130 140 150
RGSDPARQHS FIVTEELAPT VDLEVFSQDW RERPPPPRLK RALVEAVARM
160 170 180 190 200
VGDMHRAGVN HRDCYICHFL LHTDKPVSAD DFRLSVIDLH RAQTRDATPK
210 220 230 240 250
RWRNKDLAAL YFSALDIGLT RRDKLRFLRT YFRRPLREIL RDEAGLLAWM
260
ERKAEKLYER KQRYGDLL
Length:268
Mass (Da):31,311
Last modified:March 1, 2001 - v1
Checksum:i248E8D96C78A7791
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2531K → Q in AAD12056. (PubMed:11741974)Curated

Mass spectrometryi

Molecular mass is 33544.618 Da from positions 1 - 268. Determined by MALDI. Phosphorylated protein.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF087652 Genomic DNA. Translation: AAD12056.1.
AE004091 Genomic DNA. Translation: AAG08394.1.
PIRiE83020.
RefSeqiNP_253696.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG08394; AAG08394; PA5009.
GeneIDi881540.
KEGGipae:PA5009.
PATRICi19844841. VBIPseAer58763_5249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF087652 Genomic DNA. Translation: AAD12056.1 .
AE004091 Genomic DNA. Translation: AAG08394.1 .
PIRi E83020.
RefSeqi NP_253696.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q9HUF7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA5009.

Protocols and materials databases

DNASUi 881540.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG08394 ; AAG08394 ; PA5009 .
GeneIDi 881540.
KEGGi pae:PA5009.
PATRICi 19844841. VBIPseAer58763_5249.

Organism-specific databases

PseudoCAPi PA5009.

Phylogenomic databases

eggNOGi NOG04355.
HOGENOMi HOG000126215.
InParanoidi Q9HUF7.
KOi K02848.
OMAi LGAGQEW.
OrthoDBi EOG63JR6W.
PhylomeDBi Q9HUF7.

Enzyme and pathway databases

UniPathwayi UPA00958 .

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR010440. LipoPS_kinase.
IPR017172. Lsacc_core_hep_kinase_RfaP.
[Graphical view ]
Pfami PF06293. Kdo. 1 hit.
[Graphical view ]
PIRSFi PIRSF037318. RfaP. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "WaaP of Pseudomonas aeruginosa is a novel eukaryotic type protein-tyrosine kinase as well as a sugar kinase essential for the biosynthesis of core lipopolysaccharide."
    Zhao X., Lam J.S.
    J. Biol. Chem. 277:4722-4730(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 12-36; 38-54; 84-101; 151-171; 211-237 AND 257-268, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, MASS SPECTROMETRY, DISRUPTION PHENOTYPE, PHOSPHORYLATION AT TYR-30; TYR-48; TYR-98; TYR-165; TYR-211; TYR-231; TYR-258 AND TYR-264, MUTAGENESIS OF LYS-69 AND ASP-163.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRFAP_PSEAE
AccessioniPrimary (citable) accession number: Q9HUF7
Secondary accession number(s): Q9R883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Shares similarity with eukaryotic protein kinases in the conserved tyrosine kinase functional motifs.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3