Reviewed, UniProtKB/Swiss-Prot Q9HU99 (DADA2_PSEAE)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase 2 small subunit
    EC=1.4.99.1

Gene names

Name:	dadA2
Ordered Locus Names:	PA5084

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	D-amino acid dehydrogenase 2 small subunit HAMAP MF_01202		PRO_0000166139
Regions
	Nucleotide binding	5 – 19	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HU99-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1EDE536FD784FF6F	FASTA	416	44,767
	10 20 30 40 50 60 MAQRVCIIGA GVVGLATAYA LVREGFDVTL VEARERGGLE TSYANGGQLS YRYVAPLADS 70 80 90 100 110 120 GVPAQALGWL LRNDAPLRLR PRLDPAQWRW LLGFLAACRG SLNRRNAAHL LRLALLSQRT 130 140 150 160 170 180 LQDWREEDGL DGFDWRRNGK LVAFRQPASF ARARQGLADP SAQFVLTAAE CLALEPALSA 190 200 210 220 230 240 APFVGGIHTP DEEVADCHAF CVQLAERLQA SGHCRQLHGR RVTKIVEGGA AVRGVEVGGD 250 260 270 280 290 300 LIEADHVVLA AGYRSAELML PGLRLPLYPL KGYSLTLPVG AQHRAPDTSI TDYDRKIVYA 310 320 330 340 350 360 RIGERLRIAA MVDIVGFDPG LEPARLALLR QQARDTFPQG GDFDAAVEWA GMRPATPTGV 370 380 390 400 410 PLVGNGGYRN LWLNLGHGAL GFTLACGSGR LLAEQIGRRP PSIDTTGLLP RGALAG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG08469.1.
PIR	F83010.
RefSeq	NP_253771.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880266.
GenomeReviews	Gene locus PA5084 in contig AE004091_GR.
KEGG	pae:PA5084.
NMPDR	fig|208964.1.peg.5081.
Organism-specific databases
PseudoCAP	PA5084.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HU99.
OMA	AQWRWLL.
Enzyme and pathway databases
BioCyc	PAER208964:PA5084-MON.
BRENDA	1.4.99.1. 354.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA2_PSEAE

Accession

Primary (citable) accession number: Q9HU99

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)