Reviewed, UniProtKB/Swiss-Prot Q9HU72 (LGUL_PSEAE)

Names and origin

Protein names

Recommended name:
    Lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	PA5111

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Lactoylglutathione lyase		PRO_0000287775
Sites
	Metal binding	92	1	Zinc Potential
	Metal binding	95	1	Zinc Potential
	Metal binding	107	1	Zinc Potential
	Metal binding	118	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HU72-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9C85130839474892	FASTA	176	20,215
	10 20 30 40 50 60 MSFNTEVQPG ICMEPDAITQ EYVFNHTMLR VKDPKRSLDF YSRVLGMRLL RRLDFEEGRF 70 80 90 100 110 120 SLYFLAMTRG EEVPDAVDER QRYTFGRQSV LELTHNWGSE SDDSQYHNGN QDPRGFGHIC 130 140 150 160 170 FSVPDLVAAC ERFETLGVNF VKPLDRGMKN VAFISDPDGY WVEIVQASLN GEMGRG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG08496.1.
PIR	F83006.
RefSeq	NP_253798.1.
3D structure databases
HSSP	HSSP built from PDB template 1QIP based on UniProtKB Q04760.
ModBase	Search...
Genome annotation databases
GeneID	882241.
GenomeReviews	Gene locus PA5111 in contig AE004091_GR.
KEGG	pae:PA5111.
Organism-specific databases
PseudoCAP	PA5111.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HU72.
OMA	FAYHNGN.
Enzyme and pathway databases
BioCyc	PAER208964:PA5111-MON.
BRENDA	4.4.1.5. 354.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_PSEAE

Accession

Primary (citable) accession number: Q9HU72

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)