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Protein

Glucose-1-phosphate thymidylyltransferase

Gene

rmlA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis.UniRule annotation

Catalytic activityi

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei110TTPCombined sources1
Binding sitei111Glucose-1-phosphateCombined sources1
Binding sitei146Glucose-1-phosphate; via amide nitrogenCombined sources1
Binding sitei172Glucose-1-phosphate; via carbonyl oxygenCombined sources1
Binding sitei251TTP; via amide nitrogenCombined sources1
Binding sitei255TTPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15TTPCombined sources6
Nucleotide bindingi25 – 26TTPCombined sources2
Nucleotide bindingi82 – 87TTPCombined sources6
Nucleotide bindingi114 – 119TTPCombined sources6
Nucleotide bindingi219 – 220TTPCombined sources2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

NucleotidyltransferaseUniRule annotation, Transferase

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-bindingCombined sources

Enzyme and pathway databases

BRENDAi2.7.7.24. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-1-phosphate thymidylyltransferaseUniRule annotation (EC:2.7.7.24UniRule annotation)
Gene namesi
Name:rmlAImported
Ordered Locus Names:PA5163Imported
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)Imported
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5163.

PTM / Processingi

Proteomic databases

PaxDbiQ9HU22.
PRIDEiQ9HU22.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5163.

Chemistry databases

BindingDBiQ9HU22.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXOX-ray1.66A/B/C/D/E/F/G/H1-293[»]
1FZWX-ray1.90A/B/C/D/E/F/G/H1-293[»]
1G0RX-ray1.87A/B/C/D/E/F/G/H1-293[»]
1G1LX-ray1.77A/B/C/D/E/F/G/H1-293[»]
1G23X-ray2.80A/B/C/D/E/F/G/H1-293[»]
1G2VX-ray2.60A/B/C/D/E/F/G/H1-293[»]
1G3LX-ray2.70A/B/C/D1-293[»]
3ZLKX-ray1.95A/B/C/D1-293[»]
3ZLLX-ray2.00A/B/C/D1-293[»]
4ARWX-ray2.20A/B/C/D1-293[»]
4ASJX-ray2.25A/B/C/D1-293[»]
4ASYX-ray2.30A/B/C/D1-293[»]
4B2WX-ray2.36A/B/C/D1-293[»]
4B2XX-ray1.70A/B/C/D1-293[»]
4B3UX-ray1.80A/B/C/D1-293[»]
4B42X-ray2.50A/B/C/D1-293[»]
4B4BX-ray2.10A/B/C/D1-293[»]
4B4GX-ray2.50A/B/C/D1-293[»]
4B4MX-ray2.35A/B/C/D1-293[»]
4B5BX-ray2.06A/B/C/D1-293[»]
ProteinModelPortaliQ9HU22.
SMRiQ9HU22.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HU22.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 240NTP_transferaseInterPro annotationAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni161 – 162Glucose-1-phosphate bindingCombined sources2

Sequence similaritiesi

Belongs to the glucose-1-phosphate thymidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108I19. Bacteria.
COG1209. LUCA.
HOGENOMiHOG000283473.
InParanoidiQ9HU22.
KOiK00973.
OMAiPHDAEQF.
PhylomeDBiQ9HU22.

Family and domain databases

CDDicd02538. G1P_TT_short. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005907. G1P_thy_trans_s.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF105. PTHR22572:SF105. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01207. rmlA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HU22-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKGIILAG GSGTRLHPAT LAISKQLLPV YDKPMIYYPL STLMLAGIRE
60 70 80 90 100
ILIISTPQDT PRFQQLLGDG SNWGLDLQYA VQPSPDGLAQ AFLIGESFIG
110 120 130 140 150
NDLSALVLGD NLYYGHDFHE LLGSASQRQT GASVFAYHVL DPERYGVVEF
160 170 180 190 200
DQGGKAISLE EKPLEPKSNY AVTGLYFYDQ QVVDIARDLK PSPRGELEIT
210 220 230 240 250
DVNRAYLERG QLSVEIMGRG YAWLDTGTHD SLLEAGQFIA TLENRQGLKV
260 270 280 290
ACPEEIAYRQ KWIDAAQLEK LAAPLAKNGY GQYLKRLLTE TVY
Length:293
Mass (Da):32,457
Last modified:March 1, 2001 - v1
Checksum:i1E249F4359DC0553
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08548.1.
PIRiD83000.
RefSeqiNP_253850.1. NC_002516.2.
WP_003105518.1. NZ_ASJY01000811.1.

Genome annotation databases

EnsemblBacteriaiAAG08548; AAG08548; PA5163.
GeneIDi879990.
KEGGipae:PA5163.
PATRICi19845169. VBIPseAer58763_5411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08548.1.
PIRiD83000.
RefSeqiNP_253850.1. NC_002516.2.
WP_003105518.1. NZ_ASJY01000811.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FXOX-ray1.66A/B/C/D/E/F/G/H1-293[»]
1FZWX-ray1.90A/B/C/D/E/F/G/H1-293[»]
1G0RX-ray1.87A/B/C/D/E/F/G/H1-293[»]
1G1LX-ray1.77A/B/C/D/E/F/G/H1-293[»]
1G23X-ray2.80A/B/C/D/E/F/G/H1-293[»]
1G2VX-ray2.60A/B/C/D/E/F/G/H1-293[»]
1G3LX-ray2.70A/B/C/D1-293[»]
3ZLKX-ray1.95A/B/C/D1-293[»]
3ZLLX-ray2.00A/B/C/D1-293[»]
4ARWX-ray2.20A/B/C/D1-293[»]
4ASJX-ray2.25A/B/C/D1-293[»]
4ASYX-ray2.30A/B/C/D1-293[»]
4B2WX-ray2.36A/B/C/D1-293[»]
4B2XX-ray1.70A/B/C/D1-293[»]
4B3UX-ray1.80A/B/C/D1-293[»]
4B42X-ray2.50A/B/C/D1-293[»]
4B4BX-ray2.10A/B/C/D1-293[»]
4B4GX-ray2.50A/B/C/D1-293[»]
4B4MX-ray2.35A/B/C/D1-293[»]
4B5BX-ray2.06A/B/C/D1-293[»]
ProteinModelPortaliQ9HU22.
SMRiQ9HU22.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5163.

Chemistry databases

BindingDBiQ9HU22.

Proteomic databases

PaxDbiQ9HU22.
PRIDEiQ9HU22.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08548; AAG08548; PA5163.
GeneIDi879990.
KEGGipae:PA5163.
PATRICi19845169. VBIPseAer58763_5411.

Organism-specific databases

PseudoCAPiPA5163.

Phylogenomic databases

eggNOGiENOG4108I19. Bacteria.
COG1209. LUCA.
HOGENOMiHOG000283473.
InParanoidiQ9HU22.
KOiK00973.
OMAiPHDAEQF.
PhylomeDBiQ9HU22.

Enzyme and pathway databases

BRENDAi2.7.7.24. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HU22.

Family and domain databases

CDDicd02538. G1P_TT_short. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005907. G1P_thy_trans_s.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22572:SF105. PTHR22572:SF105. 1 hit.
PfamiPF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01207. rmlA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ9HU22_PSEAE
AccessioniPrimary (citable) accession number: Q9HU22
Secondary accession number(s): Q7AYQ9
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.