dTDP-4-dehydrorhamnose 3,5-epimerase - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

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Q9HU21 (RMLC_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 77. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
dTDP-4-dehydrorhamnose 3,5-epimerase
EC=5.1.3.13

Alternative name(s):
Thymidine diphospho-4-keto-rhamnose 3,5-epimerase
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase
dTDP-L-rhamnose synthase

Gene names

Name:	rmlC
Ordered Locus Names:	PA5164

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose. Ref.3
Catalytic activity	dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-beta-L-mannose. Ref.3
Pathway	Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the dTDP-4-dehydrorhamnose 3,5-epimerase family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Isomerase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTDP-rhamnose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway extracellular polysaccharide biosynthetic process Inferred from direct assay Ref.3. Source: UniProtKB lipopolysaccharide biosynthetic process Inferred from direct assay Ref.3. Source: UniProtKB
Molecular_function	dTDP-4-dehydrorhamnose 3,5-epimerase activity Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

dTDP-4-dehydrorhamnose 3,5-epimerase

PRO_0000395350

Sites

Binding site

Substrate

Binding site

Substrate

Binding site

Substrate

Binding site

Substrate

Binding site

Substrate

Binding site

Substrate

Binding site

Substrate

Binding site

118

Substrate

Binding site

168

Substrate

Secondary structure

181

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9HU21 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F44AA1D59C1772C2
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FASTA

181

20,766

        10         20         30         40         50         60 
MKATRLAIPD VILFEPRVFG DDRGFFFESY NQRAFEEACG HPVSFVQDNH SRSARGVLRG 

        70         80         90        100        110        120 
LHYQIRQAQG KLVRATLGEV FDVAVDLRRG SPTFGQWVGE RLSAENKRQM WIPAGFAHGF 

       130        140        150        160        170        180 
VVLSEYAEFL YKTTDFWAPE HERCIVWNDP ELKIDWPLQD APLLSEKDRQ GKAFADADCF 


P

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the rmlC gene of Pseudomonas aeruginosa." Maeki M. Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation." Dong C., Major L.L., Srikannathasan V., Errey J.C., Giraud M.F., Lam J.S., Graninger M., Messner P., McNeil M.R., Field R.A., Whitfield C., Naismith J.H. J. Mol. Biol. 365:146-159(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS, CATALYTIC ACTIVITY, REACTION MECHANISM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ298594 Genomic DNA. Translation: CAC82199.1.
AE004091 Genomic DNA. Translation: AAG08549.1.

PIR

E83000.

RefSeq

NP_253851.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1RTV	X-ray	2.50	A	1-181	[»]
2IXH	X-ray	2.00	A/B	1-181	[»]
2IXI	X-ray	1.80	A/B	1-181	[»]
2IXJ	X-ray	2.54	A	1-181	[»]
2IXK	X-ray	1.70	A/B	1-181	[»]

ProteinModelPortal

Q9HU21.

SMR

Q9HU21. Positions 1-181.

ModBase

Search...

Protein-protein interaction databases

STRING

208964.PA5164.

Protocols and materials databases

DNASU

879991.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

879991.

KEGG

pae:PA5164.

PATRIC

19845171. VBIPseAer58763_5412.

Organism-specific databases

PseudoCAP

PA5164.

Phylogenomic databases

eggNOG

COG1898.

HOGENOM

HOG000227724.

K01790.

OMA

VIIEPRV.

ProtClustDB

CLSK869162.

Enzyme and pathway databases

UniPathway

UPA00030.
UPA00124.

Family and domain databases

Gene3D

2.60.120.10. 1 hit.

InterPro

IPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]

PANTHER

PTHR21047. PTHR21047. 1 hit.

Pfam

PF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]

ProDom

PD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF51182. RmlC_like_cupin. 1 hit.

TIGRFAMs

TIGR01221. rmlC. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

Q9HU21.

Entry information

Entry name

RMLC_PSEAE

Accession

Primary (citable) accession number: Q9HU21
Secondary accession number(s): Q7AYQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents