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Q9HU21

- RMLC_PSEAE

UniProt

Q9HU21 - RMLC_PSEAE

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Protein
dTDP-4-dehydrorhamnose 3,5-epimerase
Gene
rmlC, PA5164
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the epimerization of the C3' and C5'positions of dTDP-6-deoxy-D-xylo-4-hexulose, forming dTDP-6-deoxy-L-lyxo-4-hexulose.1 Publication

Catalytic activityi

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei23 – 231Substrate
Binding sitei28 – 281Substrate
Binding sitei49 – 491Substrate
Binding sitei59 – 591Substrate
Binding sitei62 – 621Substrate
Binding sitei71 – 711Substrate
Binding sitei82 – 821Substrate
Binding sitei118 – 1181Substrate
Binding sitei168 – 1681Substrate

GO - Molecular functioni

  1. dTDP-4-dehydrorhamnose 3,5-epimerase activity Source: UniProtKB

GO - Biological processi

  1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  2. extracellular polysaccharide biosynthetic process Source: UniProtKB
  3. lipopolysaccharide biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00124.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-4-dehydrorhamnose 3,5-epimerase (EC:5.1.3.13)
Alternative name(s):
Thymidine diphospho-4-keto-rhamnose 3,5-epimerase
dTDP-4-keto-6-deoxyglucose 3,5-epimerase
dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase
dTDP-L-rhamnose synthase
Gene namesi
Name:rmlC
Ordered Locus Names:PA5164
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA5164.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 181181dTDP-4-dehydrorhamnose 3,5-epimerase
PRO_0000395350Add
BLAST

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi208964.PA5164.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 54
Beta strandi11 – 155
Beta strandi18 – 214
Beta strandi24 – 318
Helixi32 – 398
Beta strandi47 – 548
Beta strandi57 – 6711
Beta strandi71 – 8616
Turni92 – 954
Beta strandi97 – 1037
Turni104 – 1063
Beta strandi109 – 1124
Beta strandi116 – 1227
Beta strandi124 – 13512
Helixi139 – 1413
Beta strandi142 – 1454
Turni150 – 1523
Helixi166 – 1694
Helixi174 – 1763

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RTVX-ray2.50A1-181[»]
2IXHX-ray2.00A/B1-181[»]
2IXIX-ray1.80A/B1-181[»]
2IXJX-ray2.54A1-181[»]
2IXKX-ray1.70A/B1-181[»]
ProteinModelPortaliQ9HU21.
SMRiQ9HU21. Positions 1-181.

Miscellaneous databases

EvolutionaryTraceiQ9HU21.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1898.
HOGENOMiHOG000227724.
KOiK01790.
OMAiSERSIIW.
OrthoDBiEOG6HTP2V.
PhylomeDBiQ9HU21.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR21047. PTHR21047. 1 hit.
PfamiPF00908. dTDP_sugar_isom. 1 hit.
[Graphical view]
ProDomiPD001462. dTDP_sugar_isom. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01221. rmlC. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HU21-1 [UniParc]FASTAAdd to Basket

« Hide

MKATRLAIPD VILFEPRVFG DDRGFFFESY NQRAFEEACG HPVSFVQDNH    50
SRSARGVLRG LHYQIRQAQG KLVRATLGEV FDVAVDLRRG SPTFGQWVGE 100
RLSAENKRQM WIPAGFAHGF VVLSEYAEFL YKTTDFWAPE HERCIVWNDP 150
ELKIDWPLQD APLLSEKDRQ GKAFADADCF P 181
Length:181
Mass (Da):20,766
Last modified:March 1, 2001 - v1
Checksum:iF44AA1D59C1772C2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ298594 Genomic DNA. Translation: CAC82199.1.
AE004091 Genomic DNA. Translation: AAG08549.1.
PIRiE83000.
RefSeqiNP_253851.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG08549; AAG08549; PA5164.
GeneIDi879991.
KEGGipae:PA5164.
PATRICi19845171. VBIPseAer58763_5412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ298594 Genomic DNA. Translation: CAC82199.1 .
AE004091 Genomic DNA. Translation: AAG08549.1 .
PIRi E83000.
RefSeqi NP_253851.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RTV X-ray 2.50 A 1-181 [» ]
2IXH X-ray 2.00 A/B 1-181 [» ]
2IXI X-ray 1.80 A/B 1-181 [» ]
2IXJ X-ray 2.54 A 1-181 [» ]
2IXK X-ray 1.70 A/B 1-181 [» ]
ProteinModelPortali Q9HU21.
SMRi Q9HU21. Positions 1-181.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA5164.

Protocols and materials databases

DNASUi 879991.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG08549 ; AAG08549 ; PA5164 .
GeneIDi 879991.
KEGGi pae:PA5164.
PATRICi 19845171. VBIPseAer58763_5412.

Organism-specific databases

PseudoCAPi PA5164.

Phylogenomic databases

eggNOGi COG1898.
HOGENOMi HOG000227724.
KOi K01790.
OMAi SERSIIW.
OrthoDBi EOG6HTP2V.
PhylomeDBi Q9HU21.

Enzyme and pathway databases

UniPathwayi UPA00030 .
UPA00124 .

Miscellaneous databases

EvolutionaryTracei Q9HU21.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR000888. dTDP_sugar_isom.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
PANTHERi PTHR21047. PTHR21047. 1 hit.
Pfami PF00908. dTDP_sugar_isom. 1 hit.
[Graphical view ]
ProDomi PD001462. dTDP_sugar_isom. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SUPFAMi SSF51182. SSF51182. 1 hit.
TIGRFAMsi TIGR01221. rmlC. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of the rmlC gene of Pseudomonas aeruginosa."
    Maeki M.
    Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "RmlC, a C3' and C5' carbohydrate epimerase, appears to operate via an intermediate with an unusual twist boat conformation."
    Dong C., Major L.L., Srikannathasan V., Errey J.C., Giraud M.F., Lam J.S., Graninger M., Messner P., McNeil M.R., Field R.A., Whitfield C., Naismith J.H.
    J. Mol. Biol. 365:146-159(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) IN COMPLEX WITH SUBSTRATE, FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS, CATALYTIC ACTIVITY, REACTION MECHANISM.

Entry informationi

Entry nameiRMLC_PSEAE
AccessioniPrimary (citable) accession number: Q9HU21
Secondary accession number(s): Q7AYQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 13, 2010
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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