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Protein

Murein tetrapeptide carboxypeptidase

Gene

PA5198

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Releases the terminal D-alanine residue from the cytoplasmic disaccharide-tetrapeptide GlcNAc-MurNAc-L-Ala-gamma-D-Glu-meso-Dap-D-Ala, which is a murein turnover product. Probably also act on free tetrapetide. May be involved in murein recycling.

Catalytic activityi

GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H2O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine.1 Publication

Pathwayi: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115Nucleophile1 Publication1
Active sitei217Charge relay system1 Publication1
Active sitei285Charge relay system1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease, Serine protease

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BRENDAi3.4.17.13. 5087.
UniPathwayiUPA00544.

Protein family/group databases

MEROPSiS66.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Murein tetrapeptide carboxypeptidase (EC:3.4.17.13)
Alternative name(s):
LD-carboxypeptidase
Gene namesi
Ordered Locus Names:PA5198
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5198.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi115S → A: Loss of activity. 1 Publication1
Mutagenesisi217E → A: Loss of activity. 1 Publication1
Mutagenesisi285H → A: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003889731 – 307Murein tetrapeptide carboxypeptidaseAdd BLAST307

Proteomic databases

PaxDbiQ9HTZ1.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5198.

Structurei

Secondary structure

1307
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi16 – 21Combined sources6
Beta strandi23 – 25Combined sources3
Helixi29 – 41Combined sources13
Beta strandi46 – 48Combined sources3
Turni50 – 53Combined sources4
Beta strandi59 – 61Combined sources3
Helixi63 – 75Combined sources13
Beta strandi79 – 85Combined sources7
Helixi91 – 94Combined sources4
Turni95 – 97Combined sources3
Helixi100 – 105Combined sources6
Beta strandi111 – 113Combined sources3
Helixi115 – 117Combined sources3
Helixi118 – 126Combined sources9
Beta strandi131 – 133Combined sources3
Helixi137 – 141Combined sources5
Helixi148 – 165Combined sources18
Beta strandi171 – 173Combined sources3
Beta strandi175 – 179Combined sources5
Beta strandi184 – 192Combined sources9
Helixi193 – 197Combined sources5
Turni198 – 201Combined sources4
Beta strandi213 – 220Combined sources8
Helixi223 – 236Combined sources14
Helixi239 – 241Combined sources3
Beta strandi243 – 251Combined sources9
Helixi262 – 272Combined sources11
Beta strandi277 – 279Combined sources3
Beta strandi284 – 287Combined sources4
Beta strandi292 – 294Combined sources3
Beta strandi297 – 301Combined sources5
Beta strandi304 – 307Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZL0X-ray1.10A/B1-307[»]
1ZRSX-ray1.50A/B1-307[»]
2AUMX-ray2.40A/B1-307[»]
2AUNX-ray2.40A/B1-307[»]
ProteinModelPortaliQ9HTZ1.
SMRiQ9HTZ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HTZ1.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S66 family.Curated

Phylogenomic databases

eggNOGiENOG4107WMG. Bacteria.
COG1619. LUCA.
HOGENOMiHOG000025480.
InParanoidiQ9HTZ1.
KOiK01297.
OMAiDHELPVQ.
PhylomeDBiQ9HTZ1.

Family and domain databases

Gene3Di3.40.50.10740. 1 hit.
3.50.30.60. 1 hit.
InterProiIPR027461. Carboxypeptidase_A_C.
IPR029062. Class_I_gatase-like.
IPR027478. LdcA_N_dom.
IPR003507. S66_fam.
[Graphical view]
PfamiPF02016. Peptidase_S66. 1 hit.
[Graphical view]
PIRSFiPIRSF028757. LD-carboxypeptidase. 1 hit.
SUPFAMiSSF141986. SSF141986. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HTZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRPSSDQT WQPIDGRVAL IAPASAIATD VLEATLRQLE VHGVDYHLGR
60 70 80 90 100
HVEARYRYLA GTVEQRLEDL HNAFDMPDIT AVWCLRGGYG CGQLLPGLDW
110 120 130 140 150
GRLQAASPRP LIGFSDISVL LSAFHRHGLP AIHGPVATGL GLSPLSAPRE
160 170 180 190 200
QQERLASLAS VSRLLAGIDH ELPVQHLGGH KQRVEGALIG GNLTALACMA
210 220 230 240 250
GTLGGLHAPA GSILVLEDVG EPYYRLERSL WQLLESIDAR QLGAICLGSF
260 270 280 290 300
TDCPRKEVAH SLERIFGEYA AAIEVPLYHH LPSGHGAQNR AWPYGKTAVL

EGNRLRW
Length:307
Mass (Da):33,288
Last modified:March 1, 2001 - v1
Checksum:i0E6BA75C30DC8C9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08583.1.
PIRiE82997.
RefSeqiNP_253885.1. NC_002516.2.
WP_003100070.1. NZ_ASJY01000813.1.

Genome annotation databases

EnsemblBacteriaiAAG08583; AAG08583; PA5198.
GeneIDi880289.
KEGGipae:PA5198.
PATRICi19845243. VBIPseAer58763_5448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08583.1.
PIRiE82997.
RefSeqiNP_253885.1. NC_002516.2.
WP_003100070.1. NZ_ASJY01000813.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZL0X-ray1.10A/B1-307[»]
1ZRSX-ray1.50A/B1-307[»]
2AUMX-ray2.40A/B1-307[»]
2AUNX-ray2.40A/B1-307[»]
ProteinModelPortaliQ9HTZ1.
SMRiQ9HTZ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5198.

Protein family/group databases

MEROPSiS66.001.

Proteomic databases

PaxDbiQ9HTZ1.

Protocols and materials databases

DNASUi880289.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08583; AAG08583; PA5198.
GeneIDi880289.
KEGGipae:PA5198.
PATRICi19845243. VBIPseAer58763_5448.

Organism-specific databases

PseudoCAPiPA5198.

Phylogenomic databases

eggNOGiENOG4107WMG. Bacteria.
COG1619. LUCA.
HOGENOMiHOG000025480.
InParanoidiQ9HTZ1.
KOiK01297.
OMAiDHELPVQ.
PhylomeDBiQ9HTZ1.

Enzyme and pathway databases

UniPathwayiUPA00544.
BRENDAi3.4.17.13. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HTZ1.

Family and domain databases

Gene3Di3.40.50.10740. 1 hit.
3.50.30.60. 1 hit.
InterProiIPR027461. Carboxypeptidase_A_C.
IPR029062. Class_I_gatase-like.
IPR027478. LdcA_N_dom.
IPR003507. S66_fam.
[Graphical view]
PfamiPF02016. Peptidase_S66. 1 hit.
[Graphical view]
PIRSFiPIRSF028757. LD-carboxypeptidase. 1 hit.
SUPFAMiSSF141986. SSF141986. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLDC_PSEAE
AccessioniPrimary (citable) accession number: Q9HTZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.