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Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=1.40 mM for D-alanine1 Publication
  2. KM=1.40 mM for L-alanine1 Publication
  1. Vmax=134 µmol/min/mg enzyme toward D-alanine1 Publication
  2. Vmax=155 µmol/min/mg enzyme toward L-alanine1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei33Proton acceptor; specific for D-alanineUniRule annotation1
Binding sitei129Substrate1 Publication1
Active sitei253Proton acceptor; specific for L-alanineUniRule annotation1
Binding sitei301Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

  • alanine racemase activity Source: PseudoCAP

GO - Biological processi

Keywordsi

Molecular functionIsomerase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-5423-MONOMER
BRENDAi5.1.1.1 5087
SABIO-RKQ9HTQ2

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, catabolic1 Publication (EC:5.1.1.11 Publication)
Gene namesi
Name:dadX1 PublicationImported
Ordered Locus Names:PA5302
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5302

Pathology & Biotechi

Chemistry databases

DrugBankiDB03252 D-Lysine
DB03801 Lysine Nz-Carboxylic Acid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145471 – 357Alanine racemase, catabolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-(pyridoxal phosphate)lysine1 Publication1
Modified residuei122N6-carboxylysine1 Publication1

Proteomic databases

PaxDbiQ9HTQ2

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5302

Structurei

Secondary structure

1357
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi10 – 24Combined sources15
Beta strandi26 – 31Combined sources6
Helixi33 – 37Combined sources5
Helixi41 – 48Combined sources8
Turni49 – 51Combined sources3
Beta strandi53 – 59Combined sources7
Helixi60 – 68Combined sources9
Beta strandi75 – 77Combined sources3
Helixi84 – 86Combined sources3
Helixi87 – 92Combined sources6
Beta strandi95 – 99Combined sources5
Helixi102 – 110Combined sources9
Beta strandi117 – 123Combined sources7
Beta strandi125 – 127Combined sources3
Beta strandi129 – 132Combined sources4
Helixi134 – 146Combined sources13
Beta strandi150 – 156Combined sources7
Helixi169 – 181Combined sources13
Helixi193 – 198Combined sources6
Beta strandi205 – 207Combined sources3
Helixi211 – 214Combined sources4
Beta strandi218 – 221Combined sources4
Helixi226 – 228Combined sources3
Beta strandi233 – 245Combined sources13
Beta strandi250 – 252Combined sources3
Helixi253 – 255Combined sources3
Beta strandi260 – 269Combined sources10
Helixi272 – 274Combined sources3
Beta strandi285 – 288Combined sources4
Beta strandi291 – 295Combined sources5
Beta strandi304 – 307Combined sources4
Beta strandi319 – 327Combined sources9
Helixi329 – 335Combined sources7
Helixi340 – 345Combined sources6
Beta strandi352 – 355Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RCQX-ray1.45A1-357[»]
ProteinModelPortaliQ9HTQ2
SMRiQ9HTQ2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HTQ2

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJ4 Bacteria
COG0787 LUCA
HOGENOMiHOG000031446
InParanoidiQ9HTQ2
KOiK01775
OMAiWEILCGF
PhylomeDBiQ9HTQ2

Family and domain databases

Gene3Di2.40.37.10, 2 hits
3.20.20.10, 1 hit
HAMAPiMF_01201 Ala_racemase, 1 hit
InterProiView protein in InterPro
IPR000821 Ala_racemase
IPR009006 Ala_racemase/Decarboxylase_C
IPR011079 Ala_racemase_C
IPR001608 Ala_racemase_N
IPR020622 Ala_racemase_pyridoxalP-BS
IPR029066 PLP-binding_barrel
PfamiView protein in Pfam
PF00842 Ala_racemase_C, 1 hit
PF01168 Ala_racemase_N, 1 hit
PRINTSiPR00992 ALARACEMASE
SMARTiView protein in SMART
SM01005 Ala_racemase_C, 1 hit
SUPFAMiSSF50621 SSF50621, 1 hit
SSF51419 SSF51419, 1 hit
TIGRFAMsiTIGR00492 alr, 1 hit
PROSITEiView protein in PROSITE
PS00395 ALANINE_RACEMASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HTQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPARALIDL QALRHNYRLA REATGARALA VIKADAYGHG AVRCAEALAA
60 70 80 90 100
EADGFAVACI EEGLELREAG IRQPILLLEG FFEASELELI VAHDFWCVVH
110 120 130 140 150
CAWQLEAIER ASLARPLNVW LKMDSGMHRV GFFPEDFSAA HERLRASGKV
160 170 180 190 200
AKIVMMSHFS RADELDCPRT EEQLAAFAAA SQGLEGEISL RNSPAVLGWP
210 220 230 240 250
KVPSDWVRPG ILLYGATPFE RAHPLADRLR PVMTLESKVI SVRDLPAGEP
260 270 280 290 300
VGYGARYSTE RSQRIGVVAM GYADGYPRHA ADGTLVFIDG KPGRLVGRVS
310 320 330 340 350
MDMLTVDLTD HPQAGLGSRV ELWGPNVPVG ALAAQFGSIP YQLLCNLKRV

PRVYSGA
Length:357
Mass (Da):38,915
Last modified:March 1, 2001 - v1
Checksum:i199F4021D02FFF48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138S → R in AAD47081 (PubMed:10977898).Curated1
Sequence conflicti178A → S in AAD47081 (PubMed:10977898).Curated1
Sequence conflicti262S → R in AAD47081 (PubMed:10977898).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165881 Genomic DNA Translation: AAD47081.1
AE004091 Genomic DNA Translation: AAG08687.1
PIRiF82982
RefSeqiNP_253989.1, NC_002516.2
WP_003114351.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG08687; AAG08687; PA5302
GeneIDi878055
KEGGipae:PA5302
PATRICifig|208964.12.peg.5556

Similar proteinsi

Entry informationi

Entry nameiALR2_PSEAE
AccessioniPrimary (citable) accession number: Q9HTQ2
Secondary accession number(s): Q9S419
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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