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Protein

Alanine racemase, catabolic

Gene

dadX

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

pyridoxal 5'-phosphate1 Publication

Kineticsi

  1. KM=1.40 mM for D-alanine1 Publication
  2. KM=1.40 mM for L-alanine1 Publication
  1. Vmax=134 µmol/min/mg enzyme toward D-alanine1 Publication
  2. Vmax=155 µmol/min/mg enzyme toward L-alanine1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei33Proton acceptor; specific for D-alanineUniRule annotation1
Binding sitei129Substrate1 Publication1
Active sitei253Proton acceptor; specific for L-alanineUniRule annotation1
Binding sitei301Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi5.1.1.1. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, catabolic1 Publication (EC:5.1.1.11 Publication)
Gene namesi
Name:dadX1 PublicationImported
Ordered Locus Names:PA5302
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5302.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001145471 – 357Alanine racemase, catabolicAdd BLAST357

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33N6-(pyridoxal phosphate)lysine1 Publication1
Modified residuei122N6-carboxylysine1 Publication1

Proteomic databases

PaxDbiQ9HTQ2.
PRIDEiQ9HTQ2.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA5302.

Structurei

Secondary structure

1357
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Helixi10 – 24Combined sources15
Beta strandi26 – 31Combined sources6
Helixi33 – 37Combined sources5
Helixi41 – 48Combined sources8
Turni49 – 51Combined sources3
Beta strandi53 – 59Combined sources7
Helixi60 – 68Combined sources9
Beta strandi75 – 77Combined sources3
Helixi84 – 86Combined sources3
Helixi87 – 92Combined sources6
Beta strandi95 – 99Combined sources5
Helixi102 – 110Combined sources9
Beta strandi117 – 123Combined sources7
Beta strandi125 – 127Combined sources3
Beta strandi129 – 132Combined sources4
Helixi134 – 146Combined sources13
Beta strandi150 – 156Combined sources7
Helixi169 – 181Combined sources13
Helixi193 – 198Combined sources6
Beta strandi205 – 207Combined sources3
Helixi211 – 214Combined sources4
Beta strandi218 – 221Combined sources4
Helixi226 – 228Combined sources3
Beta strandi233 – 245Combined sources13
Beta strandi250 – 252Combined sources3
Helixi253 – 255Combined sources3
Beta strandi260 – 269Combined sources10
Helixi272 – 274Combined sources3
Beta strandi285 – 288Combined sources4
Beta strandi291 – 295Combined sources5
Beta strandi304 – 307Combined sources4
Beta strandi319 – 327Combined sources9
Helixi329 – 335Combined sources7
Helixi340 – 345Combined sources6
Beta strandi352 – 355Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RCQX-ray1.45A1-357[»]
ProteinModelPortaliQ9HTQ2.
SMRiQ9HTQ2.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HTQ2.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HTQ2.
KOiK01775.
OMAiTHAKNQM.
PhylomeDBiQ9HTQ2.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HTQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPARALIDL QALRHNYRLA REATGARALA VIKADAYGHG AVRCAEALAA
60 70 80 90 100
EADGFAVACI EEGLELREAG IRQPILLLEG FFEASELELI VAHDFWCVVH
110 120 130 140 150
CAWQLEAIER ASLARPLNVW LKMDSGMHRV GFFPEDFSAA HERLRASGKV
160 170 180 190 200
AKIVMMSHFS RADELDCPRT EEQLAAFAAA SQGLEGEISL RNSPAVLGWP
210 220 230 240 250
KVPSDWVRPG ILLYGATPFE RAHPLADRLR PVMTLESKVI SVRDLPAGEP
260 270 280 290 300
VGYGARYSTE RSQRIGVVAM GYADGYPRHA ADGTLVFIDG KPGRLVGRVS
310 320 330 340 350
MDMLTVDLTD HPQAGLGSRV ELWGPNVPVG ALAAQFGSIP YQLLCNLKRV

PRVYSGA
Length:357
Mass (Da):38,915
Last modified:March 1, 2001 - v1
Checksum:i199F4021D02FFF48
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti138S → R in AAD47081 (PubMed:10977898).Curated1
Sequence conflicti178A → S in AAD47081 (PubMed:10977898).Curated1
Sequence conflicti262S → R in AAD47081 (PubMed:10977898).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165881 Genomic DNA. Translation: AAD47081.1.
AE004091 Genomic DNA. Translation: AAG08687.1.
PIRiF82982.
RefSeqiNP_253989.1. NC_002516.2.
WP_003114351.1. NZ_ASJY01000820.1.

Genome annotation databases

EnsemblBacteriaiAAG08687; AAG08687; PA5302.
GeneIDi878055.
KEGGipae:PA5302.
PATRICi19845459. VBIPseAer58763_5556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF165881 Genomic DNA. Translation: AAD47081.1.
AE004091 Genomic DNA. Translation: AAG08687.1.
PIRiF82982.
RefSeqiNP_253989.1. NC_002516.2.
WP_003114351.1. NZ_ASJY01000820.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RCQX-ray1.45A1-357[»]
ProteinModelPortaliQ9HTQ2.
SMRiQ9HTQ2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5302.

Proteomic databases

PaxDbiQ9HTQ2.
PRIDEiQ9HTQ2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08687; AAG08687; PA5302.
GeneIDi878055.
KEGGipae:PA5302.
PATRICi19845459. VBIPseAer58763_5556.

Organism-specific databases

PseudoCAPiPA5302.

Phylogenomic databases

eggNOGiENOG4105CJ4. Bacteria.
COG0787. LUCA.
HOGENOMiHOG000031446.
InParanoidiQ9HTQ2.
KOiK01775.
OMAiTHAKNQM.
PhylomeDBiQ9HTQ2.

Enzyme and pathway databases

BRENDAi5.1.1.1. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HTQ2.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase. 1 hit.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALR2_PSEAE
AccessioniPrimary (citable) accession number: Q9HTQ2
Secondary accession number(s): Q9S419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.