Reviewed, UniProtKB/Swiss-Prot Q9HTQ0 (DADA1_PSEAE)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase 1 small subunit
    EC=1.4.99.1

Gene names

Name:	dadA1
Synonyms:	dadA
Ordered Locus Names:	PA5304

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity. HAMAP MF_01202
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase 1 small subunit HAMAP MF_01202		PRO_0000166138
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9HTQ0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: CD15DB2FB5A30215	FASTA	432	47,140
	10 20 30 40 50 60 MRVLVLGSGV IGTASAYYLA RAGFEVVVVD RQDGPALETS FANAGQVSPG YASPWAAPGI 70 80 90 100 110 120 PLKAMKWLLE KHAPLAIKLT SDPSQYAWML QMLRNCTAER YAVNKERMVR LSEYSRDCLD 130 140 150 160 170 180 ELRAETGIAY EGRTLGTTQL FRTQAQLDAA GKDIAVLERS GVPYEVLDRD GIARVEPALA 190 200 210 220 230 240 KVADKLVGAL RLPNDQTGDC QLFTTRLAEM AKGLGVEFRF GQNIERLDFA GDRINGVLVN 250 260 270 280 290 300 GELLTADHYV LALGSYSPQL LKPLGIKAPV YPLKGYSLTV PITNPEMAPT STILDETYKV 310 320 330 340 350 360 AITRFDQRIR VGGMAEIAGF DLSLNPRRRE TLEMITTDLY PEGGDISQAT FWTGLRPATP 370 380 390 400 410 420 DGTPIVGATR YRNLFLNTGH GTLGWTMACG SGRYLADLMA KKRPQISTEG LDISRYSNSP 430 ENAKNAHPAP AH

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG08689.1.
PIR	H82982.
RefSeq	NP_253991.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	878057.
GenomeReviews	Gene locus PA5304 in contig AE004091_GR.
KEGG	pae:PA5304.
Organism-specific databases
PseudoCAP	PA5304.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	MFQKHAP.
Enzyme and pathway databases
BioCyc	PAER208964:PA5304-MONOMER.
BRENDA	1.4.99.1. 354.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA1_PSEAE

Accession

Primary (citable) accession number: Q9HTQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)