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Protein

Acetylglutamate kinase

Gene

argB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Arginine biosynthesis bifunctional protein ArgJ (argJ), Amino-acid acetyltransferase (argA)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Succinylornithine transaminase/acetylornithine aminotransferase (aruC)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei33 – 331Transition state stabilizer
Binding sitei90 – 901SubstrateUniRule annotation1 Publication
Binding sitei195 – 1951SubstrateUniRule annotation1 Publication
Sitei255 – 2551Transition state stabilizer

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.2.8. 5087.
UniPathwayiUPA00068; UER00107.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylglutamate kinaseUniRule annotation (EC:2.7.2.8UniRule annotation)
Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferaseUniRule annotation
NAG kinaseUniRule annotation
Short name:
AGKUniRule annotation
Gene namesi
Name:argBUniRule annotation
Ordered Locus Names:PA5323
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5323.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 301300Acetylglutamate kinasePRO_0000112650Add
BLAST

Proteomic databases

PaxDbiQ9HTN2.

Interactioni

Subunit structurei

Homohexamer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi208964.PA5323.

Structurei

Secondary structure

1
301
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 2521Combined sources
Beta strandi29 – 346Combined sources
Turni37 – 404Combined sources
Helixi43 – 5715Combined sources
Beta strandi61 – 666Combined sources
Helixi70 – 789Combined sources
Beta strandi85 – 906Combined sources
Helixi94 – 10613Combined sources
Helixi108 – 11811Combined sources
Beta strandi123 – 1286Combined sources
Helixi130 – 1323Combined sources
Beta strandi134 – 1385Combined sources
Beta strandi157 – 1659Combined sources
Helixi167 – 1759Combined sources
Beta strandi179 – 1879Combined sources
Beta strandi193 – 1953Combined sources
Helixi198 – 20912Combined sources
Beta strandi212 – 22110Combined sources
Helixi236 – 2449Combined sources
Helixi252 – 26413Combined sources
Beta strandi268 – 2747Combined sources
Helixi280 – 2856Combined sources
Beta strandi292 – 2965Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BUFX-ray2.95A/B/C/D/E/F/G/H/I/J/K/L2-301[»]
ProteinModelPortaliQ9HTN2.
SMRiQ9HTN2. Positions 2-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9HTN2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 692Substrate binding

Sequence similaritiesi

Belongs to the acetylglutamate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CAS. Bacteria.
COG0548. LUCA.
HOGENOMiHOG000233259.
InParanoidiQ9HTN2.
KOiK00930.
OMAiPKTECCI.
OrthoDBiEOG6T1WVF.
PhylomeDBiQ9HTN2.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00082_B. ArgB_B.
InterProiIPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000728. NAGK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00761. argB. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9HTN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSRDDAAQ VAKVLSEALP YIRRFVGKTL VIKYGGNAME SEELKAGFAR
60 70 80 90 100
DVVLMKAVGI NPVVVHGGGP QIGDLLKRLS IESHFIDGMR VTDAATMDVV
110 120 130 140 150
EMVLGGQVNK DIVNLINRHG GSAIGLTGKD AELIRAKKLT VTRQTPEMTK
160 170 180 190 200
PEIIDIGHVG EVTGVNVGLL NMLVKGDFIP VIAPIGVGSN GESYNINADL
210 220 230 240 250
VAGKVAEALK AEKLMLLTNI AGLMDKQGQV LTGLSTEQVN ELIADGTIYG
260 270 280 290 300
GMLPKIRCAL EAVQGGVTSA HIIDGRVPNA VLLEIFTDSG VGTLISNRKR

H
Length:301
Mass (Da):31,849
Last modified:January 23, 2007 - v3
Checksum:iCE0B6E912305B255
GO

Mass spectrometryi

Molecular mass is 31711 Da from positions 2 - 301. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08708.1.
PIRiA82980.
RefSeqiNP_254010.1. NC_002516.2.
WP_003096572.1. NZ_ASJY01000822.1.

Genome annotation databases

EnsemblBacteriaiAAG08708; AAG08708; PA5323.
GeneIDi879620.
KEGGipae:PA5323.
PATRICi19845503. VBIPseAer58763_5578.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08708.1.
PIRiA82980.
RefSeqiNP_254010.1. NC_002516.2.
WP_003096572.1. NZ_ASJY01000822.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BUFX-ray2.95A/B/C/D/E/F/G/H/I/J/K/L2-301[»]
ProteinModelPortaliQ9HTN2.
SMRiQ9HTN2. Positions 2-299.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5323.

Proteomic databases

PaxDbiQ9HTN2.

Protocols and materials databases

DNASUi879620.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08708; AAG08708; PA5323.
GeneIDi879620.
KEGGipae:PA5323.
PATRICi19845503. VBIPseAer58763_5578.

Organism-specific databases

PseudoCAPiPA5323.

Phylogenomic databases

eggNOGiENOG4105CAS. Bacteria.
COG0548. LUCA.
HOGENOMiHOG000233259.
InParanoidiQ9HTN2.
KOiK00930.
OMAiPKTECCI.
OrthoDBiEOG6T1WVF.
PhylomeDBiQ9HTN2.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00107.
BRENDAi2.7.2.8. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9HTN2.

Family and domain databases

Gene3Di3.40.1160.10. 1 hit.
HAMAPiMF_00082_B. ArgB_B.
InterProiIPR004662. AcgluKinase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000728. NAGK. 1 hit.
PRINTSiPR00474. GLU5KINASE.
SUPFAMiSSF53633. SSF53633. 1 hit.
TIGRFAMsiTIGR00761. argB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Towards structural understanding of feedback control of arginine biosynthesis: cloning and expression of the gene for the arginine-inhibited N-acetyl-L-glutamate kinase from Pseudomonas aeruginosa, purification and crystallization of the recombinant enzyme and preliminary X-ray studies."
    Fernandez-Murga M.L., Ramon-Maiques S., Gil-Ortiz F., Fita I., Rubio V.
    Acta Crystallogr. D 58:1045-1047(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-18, MASS SPECTROMETRY, CRYSTALLIZATION.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  3. "Structural bases of feed-back control of arginine biosynthesis, revealed by the structures of two hexameric N-acetylglutamate kinases, from Thermotoga maritima and Pseudomonas aeruginosa."
    Ramon-Maiques S., Fernandez-Murga M.L., Gil-Ortiz F., Vagin A., Fita I., Rubio V.
    J. Mol. Biol. 356:695-713(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.95 ANGSTROMS) IN COMPLEX WITH SUBSTRATE AND ATP ANALOG, SUBUNIT.

Entry informationi

Entry nameiARGB_PSEAE
AccessioniPrimary (citable) accession number: Q9HTN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: January 23, 2007
Last modified: February 17, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.