Reviewed, UniProtKB/Swiss-Prot Q9HTE9 (GLYA1_PSEAE)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 1
      Short name=Serine methylase 1
      Short name=SHMT 1
    EC=2.1.2.1

Gene names

Name:	glyA1
Ordered Locus Names:	PA5415

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 1 HAMAP MF_00051		PRO_0000113637
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HTE9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9FDD4C6B7096B9D3	FASTA	417	44,700
	10 20 30 40 50 60 MFSKHDQIRG YDDELLAAMD AEEARQEDHL ELIASENYTS KRVMQAQGSG LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEH VDKVERLAID RARQLFGADY ANVQPHSGSS ANAAVYLALL NAGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG AKVSSSGKLY NAVQYGLDTA TGLIDYDEVE RLAVEHKPKM IVAGFSAYSK 190 200 210 220 230 240 TLDFPRFRAI ADKVGALLFV DMAHVAGLVA AGLYPNPIPF ADVVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LARANEEIEK KLNSAVFPGA QGGPLMHVIA AKAVCFKEAL EPGFKDYQAQ VIRNAKAMAE 310 320 330 340 350 360 VFIGRGYDVV SGGTDNHLML ISLVRQGLTG KEADAALGRV GITVNKNAVP NDPQSPFVTS 370 380 390 400 410 GIRIGTPAIT TRGLQEAQSR ELAGWICDIL DHLGDADVEA KVATQVAGLC ADFPVYR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG08800.1.
PIR	G82968.
RefSeq	NP_254102.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
SMR	Q9HTE9. Positions 1-416.
ModBase	Search...
Genome annotation databases
GeneID	880066.
GenomeReviews	Gene locus PA5415 in contig AE004091_GR.
KEGG	pae:PA5415.
NMPDR	fig|208964.1.peg.5412.
Organism-specific databases
PseudoCAP	PA5415.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HTE9.
OMA	QVIDNAQ.
Enzyme and pathway databases
BioCyc	PAER208964:PA5415-MON.
BRENDA	2.1.2.1. 354.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA1_PSEAE

Accession

Primary (citable) accession number: Q9HTE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)