ID   FADH_PSEAE              Reviewed;         399 AA.
AC   Q9HTE3;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   16-JUN-2009, entry version 51.
DE   RecName: Full=Glutathione-independent formaldehyde dehydrogenase;
DE            Short=FALDH;
DE            Short=FDH;
DE            EC=1.2.1.46;
GN   Name=fdhA; OrderedLocusNames=PA5421;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228;
RX   MEDLINE=20437337; PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T.,
RA   Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an
RT   opportunistic pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the oxidation of long-chain alcohols but is
CC       inactive against ethanol (By similarity).
CC   -!- CATALYTIC ACTIVITY: Formaldehyde + NAD(+) + H(2)O = formate +
CC       NADH.
CC   -!- CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
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DR   EMBL; AE004091; AAG08806.1; -; Genomic_DNA.
DR   PIR; E82969; E82969.
DR   RefSeq; NP_254108.1; -.
DR   HSSP; P46154; 1KOL.
DR   SMR; Q9HTE3; 3-398.
DR   GeneID; 880053; -.
DR   GenomeReviews; AE004091_GR; PA5421.
DR   KEGG; pae:PA5421; -.
DR   PseudoCAP; PA5421; -.
DR   HOGENOM; Q9HTE3; -.
DR   OMA; Q9HTE3; VACGRCR.
DR   BioCyc; PAER208964:PA5421-MON; -.
DR   BRENDA; 1.2.1.46; 354.
DR   GO; GO:0018467; F:formaldehyde dehydrogenase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR002085; ADH_SF_Zn.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   InterPro; IPR014184; HCHO_DH_non_GSH.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   TIGRFAMs; TIGR02819; fdhA_non_GSH; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase; Zinc.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    399       Glutathione-independent formaldehyde
FT                                dehydrogenase.
FT                                /FTId=PRO_0000287742.
FT   REGION      172    338       Cofactor-binding.
FT   METAL        47     47       Zinc 1; catalytic (By similarity).
FT   METAL        68     68       Zinc 1; catalytic (By similarity).
FT   METAL        98     98       Zinc 2 (By similarity).
FT   METAL       101    101       Zinc 2 (By similarity).
FT   METAL       104    104       Zinc 2 (By similarity).
FT   METAL       112    112       Zinc 2 (By similarity).
FT   METAL       177    177       Zinc 1; catalytic (By similarity).
SQ   SEQUENCE   399 AA;  42008 MW;  FE1FBAE68A90C9FC CRC64;
     MSGNRGVVYL GPGKVEVQNI PYPKMQDPQG RQIDHGVILR VVSTNICGSD QHMVRGRTTA
     PEGLVLGHEI TGEVVEIGRG VETMKIGDLV SVPFNVACGH CRTCKEQHTG VCLTVNPARA
     GGAYGYVDMG GWVGGQAEYV LVPYADFNLL KLPNREAAME KIRDLTCLSD ILPTGYHGAV
     TAGVGPGSTV YIAGAGPVGL AAAASARLLG AAVVIVGDVN PTRLAHAKKQ GFEIADLSKD
     TPLHEQIAAL LGEPEVDCAV DAVGFEARGH GHSGSQQEAP ATVLNSLMGI TRVAGKIGIP
     GLYVTEDPGA VDAAAKHGAL SIRFGLGWAK SHSFHTGQTP VMKYNRQLMQ AIMWDRIKIA
     DIVGVEVITL DDAPKGYGEF DAGVPKKFVI DPHNLFRAA
//