Reviewed, UniProtKB/Swiss-Prot Q9HTD7 (ASPA_PSEAE)

Names and origin

Protein names

Recommended name:
    Aspartate ammonia-lyase
      Short name=Aspartase
    EC=4.3.1.1

Gene names

Name:	aspA
Ordered Locus Names:	PA5429

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate = fumarate + NH3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Aspartase subfamily.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	aspartate ammonia-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	Aspartate ammonia-lyase		PRO_0000287771
Regions
	Region	144 – 146	3	Substrate binding By similarity
Sites
	Binding site	105	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HTD7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: DE868A8CA8478429	FASTA	474	51,070
	10 20 30 40 50 60 MSPVASSRIE KDLLGTLEVP ADAYYGIQTL RAVNNFRLSG VPLSHYPKLV VALAMVKQAA 70 80 90 100 110 120 ADANRQLGHL PEDKHAAISE ACARLIRGDF HEQFVVDMIQ GGAGTSTNMN ANEVIANIAL 130 140 150 160 170 180 EAMGHTKGEY KYLHPNNDVN MAQSTNDAYP TAIRLGLLLG HDTLLASLDS LIQAFAAKGV 190 200 210 220 230 240 EFAGVLKMGR TQLQDAVPMT LGQEFHAFAT TLGEDLDRLR RLAPELLTEV NLGGTAIGTG 250 260 270 280 290 300 INADPGYQKL AVERLAAISG QPLKPAADLI EATSDMGAFV LFSGMLKRTA VKLSKICNDL 310 320 330 340 350 360 RLLSSGPRTG INEINLPPRQ PGSSIMPGKV NPVIPEAVNQ VAFEVIGNDL ALTLAAEGGQ 370 380 390 400 410 420 LQLNVMEPLI AYKIFDSIRL LQRAMDMLRE HCITGITANV ERCHELVEHS IGLVTALNPY 430 440 450 460 470 IGYENSTRIA KTALESGRGV LELVREEKLL DEATLADILL PENMIAPRLI PLRA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG08814.1.
PIR	C82968.
RefSeq	NP_254116.1.
3D structure databases
HSSP	HSSP built from PDB template 1JSW based on UniProtKB P04422.
ModBase	Search...
Genome annotation databases
GeneID	878603.
GenomeReviews	Gene locus PA5429 in contig AE004091_GR.
KEGG	pae:PA5429.
Organism-specific databases
PseudoCAP	PA5429.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HTD7.
OMA	GKICAQT.
Enzyme and pathway databases
BioCyc	PAER208964:PA5429-MON.
BRENDA	4.3.1.1. 354.
Family and domain databases
InterPro	IPR004708. ApsA. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00839. aspA. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPA_PSEAE

Accession

Primary (citable) accession number: Q9HTD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)