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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei86S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei147S-adenosyl-L-methionineUniRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Gene namesi
Name:rsmGUniRule annotation
Ordered Locus Names:PA5564
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA5564.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001843061 – 214Ribosomal RNA small subunit methyltransferase GAdd BLAST214

Proteomic databases

PaxDbiQ9HT10.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA5564.

Structurei

3D structure databases

ProteinModelPortaliQ9HT10.
SMRiQ9HT10.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 133S-adenosyl-L-methionine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiQ9HT10.
KOiK03501.
OMAiQVTCLDS.
PhylomeDBiQ9HT10.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HT10-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAVTQHHAD ELARGADELG VALDADKQRQ LLAYLALLIK WNKAYNLTAV
60 70 80 90 100
RDPDEMVSRH LLDSLSIVPY AEAGDNWLDV GSGGGMPGVP LAILFPEKRL
110 120 130 140 150
TLLDSNGKKT RFLTQVKLEL KLANLEVVHS RVEAFRPESP FDGIVSRAFS
160 170 180 190 200
SLEDFANWTR HLGGQETRWL AMKGVHPNEE LAALPEDFRV EAEHALAVPG
210
CQGQRHLLIL RRTA
Length:214
Mass (Da):23,735
Last modified:March 1, 2001 - v1
Checksum:iBF90E6B9146ED0E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08949.1.
PIRiC82950.
RefSeqiNP_254251.1. NC_002516.2.
WP_003100242.1. NZ_ASJY01000843.1.

Genome annotation databases

EnsemblBacteriaiAAG08949; AAG08949; PA5564.
GeneIDi877778.
KEGGipae:PA5564.
PATRICi19846017. VBIPseAer58763_5830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG08949.1.
PIRiC82950.
RefSeqiNP_254251.1. NC_002516.2.
WP_003100242.1. NZ_ASJY01000843.1.

3D structure databases

ProteinModelPortaliQ9HT10.
SMRiQ9HT10.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA5564.

Proteomic databases

PaxDbiQ9HT10.

Protocols and materials databases

DNASUi877778.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG08949; AAG08949; PA5564.
GeneIDi877778.
KEGGipae:PA5564.
PATRICi19846017. VBIPseAer58763_5830.

Organism-specific databases

PseudoCAPiPA5564.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
InParanoidiQ9HT10.
KOiK03501.
OMAiQVTCLDS.
PhylomeDBiQ9HT10.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMG_PSEAE
AccessioniPrimary (citable) accession number: Q9HT10
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.