Reviewed, UniProtKB/Swiss-Prot Q9HSM4 (DHE42_HALSA)

Names and origin

Protein names

Recommended name:
    NADP-specific glutamate dehydrogenase B
      Short name=NADP-GDH B
    EC=1.4.1.4

Gene names

Name:	gdhB
Ordered Locus Names:	VNG_0161G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	NADP-specific glutamate dehydrogenase B		PRO_0000182780
Sites
	Active site	119	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HSM4-1 [UniParc]. Last modified January 11, 2001. Version 1. Checksum: A344F09C60BE60A6	FASTA	429	45,968
	10 20 30 40 50 60 MAQTPPPESA PSTDSEPETA LETARRQLQA AAEHVDIADG VIERLTHPTR VVQVSVPVER 70 80 90 100 110 120 DDGTVTVYDG YRAQHDDVRG PYKGGLRYHP GVSAEECVGL SMWMTWKCAV MDLPFGGAKG 130 140 150 160 170 180 GVVVDPKTLS ADEHERLTRR FAAELRDEVG PSQDIPAPDM GTDAQTMAWF MDAYSMQQGE 190 200 210 220 230 240 TVPGVVTGKP PVAGGSHGRA EAPGRSVAIA TREAINYYDI PIDDATVAVQ GYGSVGANAA 250 260 270 280 290 300 LLLDDWGARV VAVSDVNGGV LDTDGLDTHA IPSHGNQPAA VMRHDAPNTL TNEELLELDV 310 320 330 340 350 360 DVVIPAAVGN VITAANADRI QADIVVEGAN GPTTSAADRI LEERAVPVIP DILANAGGVT 370 380 390 400 410 420 VSYFEWLQDI NRRTWSPERV RDELESEMLS AWNAVRSEVD DGDLSWRDAA YVVALQRIGR AKEARGLWP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The discovery of four distinct glutamate dehydrogenase genes in a strain of Halobacterium salinarum." Ingoldsby L.M., Geoghegan K.F., Hayden B.M., Engel P.C. Gene 349:237-244(2005) [PubMed: 15780999] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRC-36014.
[2]	"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY840087 Genomic DNA. Translation: AAW19067.1. AE004437 Genomic DNA. Translation: AAG18779.1.
PIR	G84176.
RefSeq	NP_279299.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3B based on UniProtKB P96110.
ModBase	Search...
Genome annotation databases
GeneID	1447088.
GenomeReviews	Gene locus VNG_0161G in contig AE004437_GR.
KEGG	hal:VNG0161G.
NMPDR	fig|64091.1.peg.311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HSM4.
OMA	Q9HSM4. ANNKMGE.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0161G-MON.
BRENDA	1.4.1.4. 141893.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE42_HALSA

Accession

Primary (citable) accession number: Q9HSM4
Secondary accession number(s): Q5MBG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)