ID   Q9HSL0_HALSA            Unreviewed;       300 AA.
AC   Q9HSL0;
DT   01-MAR-2001, integrated into UniProtKB/TrEMBL.
DT   01-MAR-2001, sequence version 1.
DT   27-MAR-2024, entry version 136.
DE   RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE            EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN   Name=rio2 {ECO:0000313|EMBL:DAC77481.1};
GN   Synonyms=pkn1 {ECO:0000313|EMBL:DAC77481.1};
GN   OrderedLocusNames=VNG_0179C {ECO:0000313|EMBL:AAG18794.1};
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX   NCBI_TaxID=64091 {ECO:0000313|EMBL:AAG18794.1, ECO:0000313|Proteomes:UP000000554};
RN   [1] {ECO:0000313|EMBL:AAG18794.1, ECO:0000313|Proteomes:UP000000554}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1
RC   {ECO:0000313|Proteomes:UP000000554}, and NRC-1
RC   {ECO:0000313|EMBL:AAG18794.1};
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.W., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
RN   [2] {ECO:0000313|EMBL:DAC77481.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77481.1};
RX   PubMed=18313895; DOI=.1016/j.ygeno.2008.01.001;
RA   Pfeiffer F., Schuster S.C., Broicher A., Falb M., Palm P., Rodewald K.,
RA   Ruepp A., Soppa J., Tittor J., Oesterhelt D.;
RT   "Evolution in the laboratory: the genome of Halobacterium salinarum strain
RT   R1 compared to that of strain NRC-1.";
RL   Genomics 91:335-346(2008).
RN   [3] {ECO:0000313|EMBL:DAC77481.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77481.1};
RX   PubMed=26042526;
RA   Pfeiffer F., Oesterhelt D.;
RT   "A manual curation strategy to improve genome annotation: application to a
RT   set of haloarchael genomes.";
RL   Life 5:1427-1444(2015).
RN   [4] {ECO:0000313|EMBL:DAC77481.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=NRC-1 {ECO:0000313|EMBL:DAC77481.1};
RA   Pfeiffer F., Marchfelder A., Habermann B.H., Dyall-Smith M.;
RT   "The genome of the Halobacterium salinarum type strain is closely related
RT   to that of the laboratory strains NRC-1 and R1.";
RL   Microbiol. Resour. Announc. 0:0-0(2019).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC         [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00001433};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC         threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC         Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC         EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. RIO-type Ser/Thr
CC       kinase family. {ECO:0000256|ARBA:ARBA00009196}.
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DR   EMBL; AE004437; AAG18794.1; -; Genomic_DNA.
DR   EMBL; BK010829; DAC77481.1; -; Genomic_DNA.
DR   PIR; F84178; F84178.
DR   RefSeq; WP_010902089.1; NC_002607.1.
DR   AlphaFoldDB; Q9HSL0; -.
DR   STRING; 64091.VNG_0179C; -.
DR   PaxDb; 64091-VNG_0179C; -.
DR   GeneID; 68693154; -.
DR   KEGG; hal:VNG_0179C; -.
DR   PATRIC; fig|64091.14.peg.131; -.
DR   HOGENOM; CLU_018693_1_0_2; -.
DR   OrthoDB; 50101at2157; -.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR   GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0030490; P:maturation of SSU-rRNA; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd05144; RIO2_C; 1.
DR   Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR   Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR030484; Rio2.
DR   InterPro; IPR015285; RIO2_wHTH_N.
DR   InterPro; IPR000687; RIO_kinase.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR45852; SER/THR-PROTEIN KINASE RIO2; 1.
DR   PANTHER; PTHR45852:SF1; SERINE_THREONINE-PROTEIN KINASE RIO2; 1.
DR   Pfam; PF01163; RIO1; 1.
DR   Pfam; PF09202; Rio2_N; 1.
DR   SMART; SM00090; RIO; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR   SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:DAC77481.1};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000000554};
KW   Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527,
KW   ECO:0000313|EMBL:DAC77481.1};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:DAC77481.1}.
FT   DOMAIN          65..287
FT                   /note="RIO kinase"
FT                   /evidence="ECO:0000259|SMART:SM00090"
SQ   SEQUENCE   300 AA;  34255 MW;  FFB38FCE1CDF8FFC CRC64;
     MVRNVADEIA ALDPEDFHLL SGVEHGMRFS EWVSREKLPE FSRLTPQEVD YRLDRMLDRE
     FLERKTIQYE GVQLTFAGYD VLALHTFAER DSVEGFGAPL GVGKESDVYE VQSFRPMALK
     FHREGYTQFR EVHRGRDYTS EKEHTSWQYT ARKAAEREYD ALEALYPTVN VPRPVDHNRH
     AIVMEKVSGT ELSNAKLNPE QASGVLDLIL REVAAAYREG YVHADVSEYN VFVDSDGVVL
     FDWPQATPTD HENARELLAR DVENIVQYFR QKYPSDVPAV DHDAVVGAVA DDAFASVTEH
//