Q9HSH4 (GUAAA_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit A
EC=6.3.5.2

Alternative name(s):
Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	VNG_0228G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_01510
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140219
Regions
	Domain	3 – 183	181	Glutamine amidotransferase type-1
Sites
	Active site	74	1	Nucleophile By similarity
	Active site	162	1	By similarity
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HSH4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4519B00D7BE4B660	FASTA	183	19,555
	10 20 30 40 50 60 MTHILVVDNH GQFTHLEHRL LRDMDGVTVD LTDNTTPPAD IDADGLVLSG GPTMADIGHC 70 80 90 100 110 120 REYLTLDVPI LGVCLGHQLI ADELGGRIEA GDYGGYADVT VSISDADDPL VGSLAPDTRV 130 140 150 160 170 180 WASHADEVVA VPDGFTITAE SDICGVEAMG DTDRDLYGVQ WHPEVAHTEE GEAVFENFVA ICE

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG18832.1.
PIR	D84183.
RefSeq	NP_279352.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HSH4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1447142.
GenomeReviews	Gene locus VNG_0228G in contig AE004437_GR.
KEGG	hal:VNG0228G.
NMPDR	fig|64091.1.peg.364.
Phylogenomic databases
HOGENOM	HBG292341.
OMA	GQYVHRI.
PhylomeDB	Q9HSH4.
ProtClustDB	PRK00758.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0228G-MONOMER.
Family and domain databases
HAMAP	MF_01510. GMP_synthase_A. [Tree]
InterPro	IPR017926. GATASE_1. IPR004739. GMP_synth_N. IPR023686. GMP_synthase_A. [Graphical view]
KO	K01951.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_HALSA

Accession

Primary (citable) accession number: Q9HSH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program