Reviewed, UniProtKB/Swiss-Prot Q9HSH4 (GUAAA_HALSA)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit A
    EC=6.3.5.2
Alternative name(s):
    Glutamine amidotransferase

Gene names

Name:	guaAA
Ordered Locus Names:	VNG_0228G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity.
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_01510
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_01510
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	GMP synthase [glutamine-hydrolyzing] subunit A HAMAP MF_01510		PRO_0000140219
Regions
	Domain	3 – 183	181	Glutamine amidotransferase type-1
Sites
	Active site	74	1	Nucleophile By similarity
	Active site	162	1	By similarity
	Active site	164	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HSH4-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4519B00D7BE4B660	FASTA	183	19,555
	10 20 30 40 50 60 MTHILVVDNH GQFTHLEHRL LRDMDGVTVD LTDNTTPPAD IDADGLVLSG GPTMADIGHC 70 80 90 100 110 120 REYLTLDVPI LGVCLGHQLI ADELGGRIEA GDYGGYADVT VSISDADDPL VGSLAPDTRV 130 140 150 160 170 180 WASHADEVVA VPDGFTITAE SDICGVEAMG DTDRDLYGVQ WHPEVAHTEE GEAVFENFVA ICE

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004437 Genomic DNA. Translation: AAG18832.1.
PIR	D84183.
RefSeq	NP_279352.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ModBase	Search...
Genome annotation databases
GeneID	1447142.
GenomeReviews	Gene locus VNG_0228G in contig AE004437_GR.
KEGG	hal:VNG0228G.
NMPDR	fig|64091.1.peg.364.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HSH4.
OMA	GQYVHRI.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0228G-MON.
BRENDA	6.3.5.2. 141893.
Family and domain databases
HAMAP	MF_01510. [Tree]
InterPro	IPR006220. Anth_synthII. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. IPR004739. GMP_synth_N. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00096. GATASE.
TIGRFAMs	TIGR00888. guaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAAA_HALSA

Accession

Primary (citable) accession number: Q9HSH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)