Q9HSF3 (IPYR_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Synonyms:	ipp
Ordered Locus Names:	VNG_0259G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137548
Sites
	Metal binding	67	1	Magnesium 1 By similarity
	Metal binding	72	1	Magnesium 1 By similarity
	Metal binding	72	1	Magnesium 2 By similarity
	Metal binding	104	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HSF3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D23B03C1B094B2E7	FASTA	177	20,063
	10 20 30 40 50 60 MANLWEDLET GPDAPDVIYA VVECLKGERN KYEYDKDIPG VVLDRVLHSN VHYPSDYGFI 70 80 90 100 110 120 PQSYYDDGDP FDVLVLVEDQ TFPGCVIEAR PVALMEMDDD GEQDDKVIAV PEEDPRYDDV 130 140 150 160 170 EDVDDLTDQQ KAEIAEFFET YKNLEADKET AVLGWGDAQA AKDAIEHAQD LYDEQFA

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG18854.1.
PIR	B84186.
RefSeq	NP_279374.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HSF3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1447163.
GenomeReviews	Gene locus VNG_0259G in contig AE004437_GR.
KEGG	hal:VNG0259G.
NMPDR	fig|64091.1.peg.387.
Phylogenomic databases
HOGENOM	HBG529150.
OMA	PNDFNVI.
PhylomeDB	Q9HSF3.
ProtClustDB	CLSK511023.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0259G-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_HALSA

Accession

Primary (citable) accession number: Q9HSF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program