Q9HSB4 (AROD_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	VNG_0314G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	226 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.
Sequence caution	The sequence AAG18893.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 226	226	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_0000138827
Sites
	Active site	120	1	Proton acceptor By similarity
	Active site	146	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HSB4 [UniParc]. Last modified April 16, 2002. Version 2. Checksum: 0054520F1EFB9468	FASTA	226	23,048
	10 20 30 40 50 60 MEFQDFLLAA STGDLGAAPA APEHVDLVEF RMDLAADPLA ALDDYDGVLP VLATNRADWE 70 80 90 100 110 120 GGAAADGGDR IDALAEAART DCVAAVDIER SALVDDDTAD GAEALAAARS TDTTTVVSAH 130 140 150 160 170 180 DFDGTPSLSA MADLLGEACS LGDVGKLAVT PQDRGDALDV IRVTHEYSAA GMTVATMGMG 190 200 210 220 DLGRHTRAVT PLYGSKLGYA PVADGETTAP GQYAPAALQA LIADLQ

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG18893.1. Different initiation.
PIR	A84191.
RefSeq	NP_279413.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HSB4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1447200.
GenomeReviews	Gene locus VNG_0314G in contig AE004437_GR.
KEGG	hal:VNG0314G.
NMPDR	fig|64091.1.peg.426.
Phylogenomic databases
HOGENOM	HBG732926.
ProtClustDB	CLSK511042.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0314G-MONOMER.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_HALSA

Accession

Primary (citable) accession number: Q9HSB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	April 16, 2002
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program