Reviewed, UniProtKB/Swiss-Prot Q9HRF0 (PANE_HALSA)

Names and origin

Protein names

Recommended name:
    Putative 2-dehydropantoate 2-reductase
    EC=1.1.1.169
Alternative name(s):
    Ketopantoate reductase
      Short name=KPA reductase
      Short name=KPR

Gene names

Ordered Locus Names:

VNG_0730C

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP or NADPH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Putative 2-dehydropantoate 2-reductase		PRO_0000157323
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	185	1	Proton donor By similarity
	Binding site	103	1	NADP; via amide nitrogen By similarity
	Binding site	103	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity
	Binding site	193	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	267	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HRF0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B373143F2822DC94	FASTA	303	31,124
	10 20 30 40 50 60 MRVVVQGPGS LGSLVGGVLA GGETAVTLLG HQSEHLTRVR EDGLRVVQPD GTTRVTRPSV 70 80 90 100 110 120 ATDPSVVADA DLVVVCVKSY DTASAARALG RQCDGAMVLT LQNGLGNAAV LAEHVPADTV 130 140 150 160 170 180 LVGTTTHGAA RTEPGVVRHA GGGETTIGRY RGANDARVAS VAAAFSTGGM ETTVTASPQR 190 200 210 220 230 240 AVWEKVLVNV GINAATALAD VDNGALVECP PGERVLERAV TEGVRVAEAE GVSVSESVVE 250 260 270 280 290 300 RARQVAARTA SNESSMRQDL AGGARTEVES LHGAVVERAR DHDIAVPVIR TLADLVRLAQ RDG

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004437 Genomic DNA. Translation: AAG19208.1.
PIR	D84230.
RefSeq	NP_279728.1.
3D structure databases
HSSP	HSSP built from PDB template 1KS9 based on UniProtKB P77728.
ModBase	Search...
Genome annotation databases
GeneID	1447514.
GenomeReviews	Gene locus VNG_0730C in contig AE004437_GR.
KEGG	hal:VNG0730C.
NMPDR	fig|64091.1.peg.744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HRF0.
OMA	TANINPH.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0730C-MON.
BRENDA	1.1.1.169. 141893.
Family and domain databases
InterPro	IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR21708:SF21. ApbA. 1 hit.
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANE_HALSA

Accession

Primary (citable) accession number: Q9HRF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2002
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)