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Protein

Putative 2-dehydropantoate 2-reductase

Gene

VNG_0730C

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.By similarity

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathway: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. Putative 2-dehydropantoate 2-reductase (VNG_0730C)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031NADP; via amide nitrogenBy similarity
Binding sitei103 – 1031SubstrateBy similarity
Active sitei185 – 1851Proton donorBy similarity
Binding sitei189 – 1891SubstrateBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei255 – 2551SubstrateBy similarity
Binding sitei267 – 2671NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi7 – 126NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pantothenate biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Ordered Locus Names:VNG_0730C
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Putative 2-dehydropantoate 2-reductasePRO_0000157323Add
BLAST

Proteomic databases

PaxDbiQ9HRF0.

Interactioni

Protein-protein interaction databases

STRINGi64091.VNG0730C.

Structurei

3D structure databases

ProteinModelPortaliQ9HRF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiCOG1893.
InParanoidiQ9HRF0.
KOiK00077.
OMAiNILGTMW.
PhylomeDBiQ9HRF0.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HRF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVVVQGPGS LGSLVGGVLA GGETAVTLLG HQSEHLTRVR EDGLRVVQPD
60 70 80 90 100
GTTRVTRPSV ATDPSVVADA DLVVVCVKSY DTASAARALG RQCDGAMVLT
110 120 130 140 150
LQNGLGNAAV LAEHVPADTV LVGTTTHGAA RTEPGVVRHA GGGETTIGRY
160 170 180 190 200
RGANDARVAS VAAAFSTGGM ETTVTASPQR AVWEKVLVNV GINAATALAD
210 220 230 240 250
VDNGALVECP PGERVLERAV TEGVRVAEAE GVSVSESVVE RARQVAARTA
260 270 280 290 300
SNESSMRQDL AGGARTEVES LHGAVVERAR DHDIAVPVIR TLADLVRLAQ

RDG
Length:303
Mass (Da):31,124
Last modified:March 1, 2001 - v1
Checksum:iB373143F2822DC94
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG19208.1.
PIRiD84230.
RefSeqiNP_279728.1. NC_002607.1.
WP_010902504.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG19208; AAG19208; VNG_0730C.
GeneIDi1447514.
KEGGihal:VNG0730C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG19208.1.
PIRiD84230.
RefSeqiNP_279728.1. NC_002607.1.
WP_010902504.1. NC_002607.1.

3D structure databases

ProteinModelPortaliQ9HRF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64091.VNG0730C.

Proteomic databases

PaxDbiQ9HRF0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG19208; AAG19208; VNG_0730C.
GeneIDi1447514.
KEGGihal:VNG0730C.

Phylogenomic databases

eggNOGiCOG1893.
InParanoidiQ9HRF0.
KOiK00077.
OMAiNILGTMW.
PhylomeDBiQ9HRF0.

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiPANE_HALSA
AccessioniPrimary (citable) accession number: Q9HRF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2002
Last sequence update: March 1, 2001
Last modified: May 27, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.