Reviewed, UniProtKB/Swiss-Prot Q9HRE8 (RTCA_HALSA)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Synonyms:	tpc
Ordered Locus Names:	VNG_0732G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential HAMAP MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_0000156424
Sites
	Active site	310	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HRE8-1 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 27B75201F22A61FE	FASTA	339	34,879
	10 20 30 40 50 60 MHVLDGSSGG GQLVRTALTC AAVSGESFRM RYVRRARSNP GLQAQHCAAV NAVADICDAA 70 80 90 100 110 120 TDGVEVGSEA FSFEPEVAAE EADDDEEPTL GGTTSVEVGT AGSIPLVFDS LLPLAGALDE 130 140 150 160 170 180 PITATLTGGT DAKWAPPMDY FQHVKLPLLR EHGIDATVSV DRRGFYPRGG GEATLTVEPS 190 200 210 220 230 240 TPTPITLTER GDREALTAYS VAESSLADDE VAEQQATAAA PDAAHEIAYT DADSAGSAVV 250 260 270 280 290 300 LAAEYEHSRA GFAALGERGV SADAVGENAA DALAAFESGP GAVDSHLADQ LVPVVAVAGG 310 320 330 EVRAPEVTTH IETCVDLLAE FDYDIDIEHT DDGAVVLSA

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG19210.1. Different initiation.
PIR	F84230.
RefSeq	NP_279730.1.
3D structure databases
SMR	Q9HRE8. Positions 1-334.
ModBase	Search...
Genome annotation databases
GeneID	1447516.
GenomeReviews	Gene locus VNG_0732G in contig AE004437_GR.
KEGG	hal:VNG0732G.
NMPDR	fig|64091.1.peg.746.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG683539.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0732G-MONOMER.
BRENDA	6.5.1.4. 141893.
Family and domain databases
HAMAP	MF_00200. RTC. [Tree]
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_HALSA

Accession

Primary (citable) accession number: Q9HRE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	February 9, 2010
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)