Reviewed, UniProtKB/Swiss-Prot Q9HR35 (DNLI_HALSA)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	VNG_0881G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	561 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 561	561	DNA ligase HAMAP MF_00407		PRO_0000059602
Sites
	Active site	255	1	N6-AMP-lysine intermediate By similarity
	Binding site	253	1	ATP By similarity
	Binding site	260	1	ATP By similarity
	Binding site	275	1	ATP By similarity
	Binding site	304	1	ATP By similarity
	Binding site	344	1	ATP By similarity
	Binding site	421	1	ATP By similarity
	Binding site	427	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HR35-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5BB30D3C1D106D2E	FASTA	561	59,620
	10 20 30 40 50 60 MKFGSFATFA ADIEATDADL DVVALVAELF DAADSDLDVV ARFVQGRVFP AHSETKLDIG 70 80 90 100 110 120 PQLCYTALSR AAGRNVDADD IEARLAETGD IGAVAGSLDL GGQTGLAAFG GGDDHADGLT 130 140 150 160 170 180 VSGVADELAA LAAAAGDGSQ SEKVTLLFGL FNQCTSREAR YLARLVLGEM RIGVGEGAVR 190 200 210 220 230 240 DAIAEAFDVP TAAVQRALQV SNDYGLVAET ARDSGTDALD AMGLEVGRPV QAMLAQAGTV 250 260 270 280 290 300 TDALDEWHEA AVETKFDGAR VQVHYDGDDV VLYSRNMENV TGALPELVEF VENNVTAPVI 310 320 330 340 350 360 IDGEAVAADE DGDPLPFQEI LKRFRRKHDV AAMREEISVE LHAFDCLHAP SADGGEDLLD 370 380 390 400 410 420 APFSDRHRRL RSVVDDASAV SEVLVTDDAD EIAAFEATAL EGGHEGIMLK NPAAPYTPGD 430 440 450 460 470 480 RGKDWLKRKP DVETLDLVVT GAEWGEGRRA SVLGTFLLSA RDAAGDGFET IGKVATGLTD 490 500 510 520 530 540 EELAALSDRL EPHVRSEDGQ TVDIEPAVVL EVGYEEIQAS PTYSSGYALR FPRFVSVRED 550 560 KTPTTADTIE RVERLAAQQQ Q

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG19323.1.
PIR	G84244.
RefSeq	NP_279843.1.
3D structure databases
SMR	Q9HR35. Positions 1-560.
ModBase	Search...
Genome annotation databases
GeneID	1447628.
GenomeReviews	Gene locus VNG_0881G in contig AE004437_GR.
KEGG	hal:VNG0881G.
NMPDR	fig|64091.1.peg.859.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG570821.
OMA	GRPRPFQ.
Enzyme and pathway databases
BioCyc	HSP64091:VNG0881G-MONOMER.
BRENDA	6.5.1.1. 141893.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. False negative. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_HALSA

Accession

Primary (citable) accession number: Q9HR35

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)