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Q9HQU4

- IMDH_HALSA

UniProt

Q9HQU4 - IMDH_HALSA

Protein

Inosine-5'-monophosphate dehydrogenase

Gene

guaB

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (03 Apr 2013)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.UniRule annotation

    Catalytic activityi

    Inosine 5'-phosphate + NAD+ + H2O = xanthosine 5'-phosphate + NADH.UniRule annotation

    Cofactori

    Potassium.UniRule annotation

    Enzyme regulationi

    Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei260 – 2601NADUniRule annotation
    Metal bindingi310 – 3101Potassium; via carbonyl oxygenUniRule annotation
    Metal bindingi312 – 3121Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei313 – 3131IMPUniRule annotation
    Active sitei315 – 3151Thioimidate intermediateUniRule annotation
    Metal bindingi315 – 3151Potassium; via carbonyl oxygenUniRule annotation
    Binding sitei425 – 4251IMPUniRule annotation
    Metal bindingi479 – 4791Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi480 – 4801Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation
    Metal bindingi481 – 4811Potassium; via carbonyl oxygen; shared with tetrameric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi308 – 3103NADUniRule annotation

    GO - Molecular functioni

    1. adenyl nucleotide binding Source: InterPro
    2. IMP dehydrogenase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. GMP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    GMP biosynthesis, Purine biosynthesis

    Keywords - Ligandi

    Metal-binding, NAD, Potassium

    Enzyme and pathway databases

    UniPathwayiUPA00601; UER00295.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Inosine-5'-monophosphate dehydrogenaseUniRule annotation (EC:1.1.1.205UniRule annotation)
    Short name:
    IMP dehydrogenaseUniRule annotation
    Short name:
    IMPDUniRule annotation
    Short name:
    IMPDHUniRule annotation
    Gene namesi
    Name:guaBUniRule annotation
    Ordered Locus Names:VNG_1001G
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Inosine-5'-monophosphate dehydrogenasePRO_0000415692Add
    BLAST

    Proteomic databases

    PaxDbiQ9HQU4.
    PRIDEiQ9HQU4.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi64091.VNG1001G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HQU4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini106 – 16560CBS 1UniRule annotationAdd
    BLAST
    Domaini169 – 22557CBS 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni348 – 3503IMP bindingUniRule annotation
    Regioni371 – 3722IMP bindingUniRule annotation
    Regioni395 – 3995IMP bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the IMPDH/GMPR family.UniRule annotation
    Contains 2 CBS domains.UniRule annotation

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    eggNOGiCOG0517.
    KOiK00088.
    OMAiNAIRGYT.
    PhylomeDBiQ9HQU4.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01964. IMPDH.
    InterProiIPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view]
    PANTHERiPTHR11911:SF6. PTHR11911:SF6. 1 hit.
    PfamiPF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000130. IMPDH. 1 hit.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR01302. IMP_dehydrog. 1 hit.
    PROSITEiPS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HQU4-1 [UniParc]FASTAAdd to Basket

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    MANDSSTDGR FSEKLRVPEA LTFDDVLLRP AESRVEPDDA DVATRVSTNV    50
    ELEVPVLSAA MDTVTESRLA IAMAREGGLG VLHQNMDTDR VVAEVERVKR 100
    ADELVIDREN VVTAAPEQTV EAVDEMMDRS DVSGAPVVDD DDTVRGIISA 150
    TDIRPYLEVG ESDAVREAMT DEVITAPEDI TARDALELMY EHKIERVPIV 200
    NDEQHLVGLV TMQGILERRE HGSAARDQNG RLRVGVAVGP FDTERATAVD 250
    EAGADVLFID CAHAHNLNVI DSAREIKASV DADVVVGNVG TREAAEAVVD 300
    FADGIKVGIG PGSICTTRVV TGSGMPQITA VSQVADVAAP AGVPVIADGG 350
    IRYSGDAAKA IAAGADAVML GSYFAGTDEA PGRVITMNGK KYKQYRGMGS 400
    VGAMQSGGSD RYLKDDDEDE EYVPEGVEAA TPYKGSLASE LHQLVGGIQS 450
    GMGYVGAESI PAFKADAEFV RVSAAGQTEG HPHDVMITDE APNYSPQGE 499
    Length:499
    Mass (Da):52,767
    Last modified:April 3, 2013 - v2
    Checksum:i3A6C3B23A42B9D39
    GO

    Sequence cautioni

    The sequence AAG19418.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG19418.1. Different initiation.
    PIRiF84256.
    RefSeqiNP_279938.1. NC_002607.1.

    Genome annotation databases

    EnsemblBacteriaiAAG19418; AAG19418; VNG_1001G.
    GeneIDi1447720.
    KEGGihal:VNG1001G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG19418.1 . Different initiation.
    PIRi F84256.
    RefSeqi NP_279938.1. NC_002607.1.

    3D structure databases

    ProteinModelPortali Q9HQU4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG1001G.

    Proteomic databases

    PaxDbi Q9HQU4.
    PRIDEi Q9HQU4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG19418 ; AAG19418 ; VNG_1001G .
    GeneIDi 1447720.
    KEGGi hal:VNG1001G.

    Phylogenomic databases

    eggNOGi COG0517.
    KOi K00088.
    OMAi NAIRGYT.
    PhylomeDBi Q9HQU4.

    Enzyme and pathway databases

    UniPathwayi UPA00601 ; UER00295 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01964. IMPDH.
    InterProi IPR013785. Aldolase_TIM.
    IPR000644. CBS_dom.
    IPR005990. IMP_DH.
    IPR015875. IMP_DH/GMP_Rdtase_CS.
    IPR001093. IMP_DH_GMPRt.
    [Graphical view ]
    PANTHERi PTHR11911:SF6. PTHR11911:SF6. 1 hit.
    Pfami PF00571. CBS. 2 hits.
    PF00478. IMPDH. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000130. IMPDH. 1 hit.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR01302. IMP_dehydrog. 1 hit.
    PROSITEi PS51371. CBS. 2 hits.
    PS00487. IMP_DH_GMP_RED. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.

    Entry informationi

    Entry nameiIMDH_HALSA
    AccessioniPrimary (citable) accession number: Q9HQU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2012
    Last sequence update: April 3, 2013
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3