Q9HQM6 (PURA_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	VNG_1089G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095268
Regions
	Nucleotide binding	11 – 17	7	GTP By similarity
	Nucleotide binding	39 – 41	3	GTP By similarity
	Nucleotide binding	339 – 341	3	GTP By similarity
	Nucleotide binding	424 – 426	3	GTP By similarity
	Region	12 – 15	4	IMP binding By similarity
	Region	37 – 40	4	IMP binding By similarity
	Region	307 – 313	7	Substrate binding By similarity
Sites
	Active site	12	1	Proton acceptor By similarity
	Active site	40	1	Proton donor By similarity
	Metal binding	12	1	Magnesium By similarity
	Metal binding	39	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	127	1	IMP By similarity
	Binding site	141	1	IMP; shared with dimeric partner By similarity
	Binding site	230	1	IMP By similarity
	Binding site	245	1	IMP By similarity
	Binding site	311	1	IMP By similarity
	Binding site	313	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HQM6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FD36AE43C7344E4E	FASTA	440	47,110
	10 20 30 40 50 60 MTVTIVGAQL GDEGKGGVVD LFGDATDVVV RYQGGDNAGH TVVAGGEEYK LSLVPSGVVR 70 80 90 100 110 120 GKTGVLGNGC VINPETLFEE VDALRERGLD PDVRLAKRAH VILPFHRELD GAEEAAKADS 130 140 150 160 170 180 DSEIGTTGRG IGPTYEDKIG RRGVRVGDLL NEDALRDRLE YLVDAKRAIY EDVYGFDASE 190 200 210 220 230 240 TDGAFDIDAI HEQCLAYADR IRDEDLAVNA GDYLADRIAD GDNVMLEGAQ GTSLDIDHGN 250 260 270 280 290 300 FPYVTSSNPT AGYAATGSGL GPTTVGQGEI VGIIKAYLSR VGSGPMPTEL DGDQAEYIRE 310 320 330 340 350 360 EGGEYGTVTG RPRRVGWLDM PMLRHAARAN GFTGIAINHL DVLAELDEVN VGHAYERDGE 370 380 390 400 410 420 RIHTLPATTE AWRDCDPVMK SFDGWSAFDP AVVADAGYSA LPDNAQAYVE YVEAELDTPA 430 440 YVLGVGPGRE ESVIRQNPFE

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG19487.1.
PIR	C84265.
RefSeq	NP_280007.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HQM6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1447788.
GenomeReviews	Gene locus VNG_1089G in contig AE004437_GR.
KEGG	hal:VNG1089G.
NMPDR	fig|64091.1.peg.1025.
Phylogenomic databases
HOGENOM	HBG658237.
OMA	YVLGIIK.
PhylomeDB	Q9HQM6.
ProtClustDB	PRK13785.
Enzyme and pathway databases
BioCyc	HSP64091:VNG1089G-MONOMER.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_HALSA

Accession

Primary (citable) accession number: Q9HQM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program