Q9HQD6 (HUTI_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable imidazolonepropionase
EC=3.5.2.7

Alternative name(s):
Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	VNG_1211G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the HutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Probable imidazolonepropionase HAMAP MF_00372		PRO_0000160978
Sites
	Metal binding	77	1	Zinc or iron By similarity
	Metal binding	79	1	Zinc or iron By similarity
	Metal binding	244	1	Zinc or iron By similarity
	Metal binding	323	1	Zinc or iron By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	99	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	247	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HQD6 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3D2EF2B8918D3CF1	FASTA	417	42,297
	10 20 30 40 50 60 MSSLDAVVHG ARELVVGPAA GGDTLETHAD GAVAVVDGAV AAVGDTADVL AAYPAENATT 70 80 90 100 110 120 AIDATGKTVL PGFVDPHTHA LFAGDRSDEF AAKLRGKPYQ EILAEGGGIL RTVDAVRAAS 130 140 150 160 170 180 DAALVANLTA QLDVMLAHGT TTAEVKTGYG LDTETECRML DAIAAAAAEH PVDVVTTFLG 190 200 210 220 230 240 AHAVPDDTDA DAYVDAVIDD QLPAAANGPA RFCDVFCEAD VFTVEQSRRI LDAGREHGLA 250 260 270 280 290 300 PKLHAEEFTR LGGAQLAADL GATSADHLLH ATPEDAAALA DAGVTPVLLP ATAFVLDEAY 310 320 330 340 350 360 ADPQQFLAAA DNRTGAPVAL GTDLNPNCYT HSMGFVVSLA CNGMRMAPAD AVLAATAWAA 370 380 390 400 410 SALDRGRDGT GTLREGTDGD VLVVDAPSHV HLPYNPGVNN VEAVLTDGTV AVGGGGA

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG19579.1.
PIR	G84276.
RefSeq	NP_280099.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HQD6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1447878.
GenomeReviews	Gene locus VNG_1211G in contig AE004437_GR.
KEGG	hal:VNG1211G.
NMPDR	fig|64091.1.peg.1118.
Phylogenomic databases
HOGENOM	HBG686142.
OMA	MNMACTL.
ProtClustDB	PRK09356.
Enzyme and pathway databases
BioCyc	HSP64091:VNG1211G-MONOMER.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01468.
PANTHER	PTHR22642. PTHR22642. 1 hit.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR01224. HutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_HALSA

Accession

Primary (citable) accession number: Q9HQD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program