Q9HQ29 (FUMC_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	VNG_1356G

Organism

Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible addition of water to fumarate to give L-malate By similarity. HAMAP-Rule MF_00743
Catalytic activity	(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP-Rule MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular_component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular_function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Fumarate hydratase class II HAMAP-Rule MF_00743		PRO_0000161332
Regions
	Region	99 – 101	3	Substrate binding By similarity
	Region	129 – 132	4	B site By similarity
	Region	139 – 141	3	Substrate binding By similarity
	Region	187 – 188	2	Substrate binding By similarity
	Region	324 – 326	3	Substrate binding By similarity
Sites
	Active site	188	1	Proton donor/acceptor By similarity
	Active site	318	1	By similarity
	Binding site	319	1	Substrate By similarity
	Site	331	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HQ29 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 31FF468F0B4FC261	FASTA	470	49,597
	10 20 30 40 50 60 MSEDYRTEQD SLGEMQVPAD AYWGAQTQRA IENFPISGIA FGRRFVRALG VVKKAAAQAN 70 80 90 100 110 120 RDLGLVDDER ADAIVAAADE VIAGEHDDQF PVDVFQTGSG TSSNMNANEV IANRAAELLG 130 140 150 160 170 180 EEIGDRVVHP NDHVNYGQSS NDVIPTAMHV ASLDALVNDV KPGLETLAAE LDDKADAFDG 190 200 210 220 230 240 VVKTGRTHLQ DATPVRLGQE FGGYRTQVEK GIDRIEAVAP RLSELALGGT AVGTGLNTHP 250 260 270 280 290 300 EFPETAAGYI SEETGVTFRE ADNHFEAQAA HDAMNEAHGA LRTVAGSLNK IANDLRLLAS 310 320 330 340 350 360 GPRNGLGEIE QPENQPGSSI MPGKINPVVA EAVNQVHKQV VGNDAAIAAG AAEGQIDLNL 370 380 390 400 410 420 YKPVLAHNFL QSADMLANAS AAFGEKFVAK LEANEAACEA QVERSMALAT ALNPTIGYDK 430 440 450 460 470 ASEVAKAALK EGKTVTEVVV EKGYLSEAEA ADVLDPEGMT HRGILSGDDT

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG19688.1.
PIR	D84290.
RefSeq	NP_280208.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HQ29.
SMR	Q9HQ29. Positions 5-459.
ModBase	Search...
Protein-protein interaction databases
STRING	64091.VNG1356G.
Proteomic databases
PaxDb	Q9HQ29.
PRIDE	Q9HQ29.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG19688; AAG19688; VNG_1356G.
GeneID	1447987.
KEGG	hal:VNG1356G.
Phylogenomic databases
eggNOG	COG0114.
KO	K01679.
OMA	KDTMGEV.
ProtClustDB	PRK00485.
Enzyme and pathway databases
UniPathway	UPA00223; UER01007.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
HAMAP	MF_00743. FumaraseC.
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR024083. Fumarase/histidase_N. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_HALSA

Accession

Primary (citable) accession number: Q9HQ29

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program