Q9HPD3 (RL4_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L4P

Gene names

Name:	rpl4p
Ordered Locus Names:	VNG_1690G

Organism

Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome By similarity. HAMAP-Rule MF_01328 Forms part of the polypeptide exit tunnel By similarity. HAMAP-Rule MF_01328
Subunit structure	Part of the 50S ribosomal subunit.
Sequence similarities	Belongs to the ribosomal protein L4P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	50S ribosomal protein L4P HAMAP-Rule MF_01328		PRO_0000129330
Experimental info
	Sequence conflict	24 – 25	2	EP → TN in BAA22271. Ref.1
	Sequence conflict	32	1	K → N in BAA22271. Ref.1
	Sequence conflict	73 – 84	12	ANGQG…VPQTV → QTGRVRRAPDSF in BAA22271. Ref.1
	Sequence conflict	98 – 99	2	HG → RS in BAA22271. Ref.1
	Sequence conflict	108 – 109	2	KA → QL in BAA22271. Ref.1
	Sequence conflict	113 – 126	14	AAVAA…ELVAD → SALLATADHDSELA Ref.1
	Sequence conflict	173 – 174	2	GR → AA in BAA22271. Ref.1
	Sequence conflict	182	1	T → E in BAA22271. Ref.1
	Sequence conflict	207	1	Missing in BAA22271. Ref.1
	Sequence conflict	218	1	T → S in BAA22271. Ref.1
	Sequence conflict	231	1	Missing in BAA22271. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9HPD3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C546488B6DD4642E	FASTA	250	26,658
	10 20 30 40 50 60 MQVTVRDLDG DDAGTLDLPR VFEEPVRPDL VKRAVLAAQA NRTQEYGADE YAGLRTTAES 70 80 90 100 110 120 QGSGRGMAHV PKANGQGARV PQTVGGRKAH PPKAEKDHGL DVNDKERKAA VRAAVAATTD 130 140 150 160 170 180 SELVADRGHN FDDDVEFPLV VSDDFEDLVK TQDVVSLLEA LGVHADIERA DEGRTVRAGQ 190 200 210 220 230 240 GTLRGRKYQE PTSILFVTAS ESGPSTAARN LAGVDVATGR EVNAEDLAPG AEPGRLTVWT 250 ESAVEEVAQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the genes encoding the L3, L4, and L23 equivalent ribosomal proteins from the archaebacterium Halobacterium halobium." Yuki Y., Kanechika R., Itoh T. Biochim. Biophys. Acta 1216:335-338(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.
[3]	"Purification, properties, and N-terminal amino acid sequence of certain 50S ribosomal subunit proteins from the archaebacterium Halobacterium cutirubrum." Matheson A.T., Yaguchi M., Christensen P., Rollin C.F., Hasnain S. Can. J. Biochem. Cell Biol. 62:426-433(1984) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-33.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB006961 Genomic DNA. Translation: BAA22271.1. AE004437 Genomic DNA. Translation: AAG19937.1.
PIR	E84321. S43421.
RefSeq	NP_280457.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HPD3.
SMR	Q9HPD3. Positions 1-250.
ModBase	Search...
Protein-protein interaction databases
STRING	64091.VNG1690G.
Proteomic databases
PaxDb	Q9HPD3.
PRIDE	Q9HPD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG19937; AAG19937; VNG_1690G.
GeneID	1448238.
KEGG	hal:VNG1690G.
Phylogenomic databases
eggNOG	COG0088.
KO	K02930.
OMA	QGARNHP.
ProtClustDB	PRK04042.
Family and domain databases
Gene3D	3.40.1370.10. 1 hit.
HAMAP	MF_01328_A. Ribosomal_L4_A.
InterPro	IPR002136. Ribosomal_L4/L1e. IPR013000. Ribosomal_L4/L1e_euk/arc_CS. IPR023574. Ribosomal_L4_dom. IPR019970. Ribosomall_L4P. [Graphical view]
Pfam	PF00573. Ribosomal_L4. 1 hit. [Graphical view]
SUPFAM	SSF52166. Ribosomal_L4/L1E. 1 hit.
TIGRFAMs	TIGR03672. rpl4p_arch. 1 hit.
PROSITE	PS00939. RIBOSOMAL_L1E. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL4_HALSA

Accession

Primary (citable) accession number: Q9HPD3
Secondary accession number(s): P05967, Q06845

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program