Reviewed, UniProtKB/Swiss-Prot Q9HP33 (GUAAB_HALSA)

Names and origin

Protein names

Recommended name:
    GMP synthase [glutamine-hydrolyzing] subunit B
    EC=6.3.5.2
Alternative name(s):
    GMP synthetase

Gene names

Name:	guaAB
Ordered Locus Names:	VNG_1829G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the synthesis of GMP from XMP By similarity. HAMAP MF_00345
Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate. HAMAP MF_00345
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1. HAMAP MF_00345
Subunit structure	Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B) Potential. HAMAP MF_00345
Sequence similarities	Contains 1 GMP-binding domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: HAMAP NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	GMP synthase [glutamine-hydrolyzing] subunit B HAMAP MF_00345		PRO_0000140239
Regions
	Domain	33 – 186	154	GMP-binding
	Nucleotide binding	29 – 35	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HP33-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D47A25946A0869ED	FASTA	305	33,725
	10 20 30 40 50 60 MVDVDSFVED ARAEISEALG DETAIIALSG GVDSSTAAAL AYEAVGDQLV PVYVDTGLMR 70 80 90 100 110 120 KGETDEIREV FDYMDSLRVV DASDRFFDEL AGVTDPEEKR HAIGEQFIRE FETVAEEVDA 130 140 150 160 170 180 TQLVQGTIYP DRIESEGTIK SHHNVGGLPE RVGFEGIVEP MRDLYKDEVR EVARHLGLEE 190 200 210 220 230 240 IISERMPFPG PGLAVRVIGE VTPEKVAVCR EATHIVEDEL EAYEPWQAFA AVLGRATGVK 250 260 270 280 290 300 GDNRVHGHVV AVRSVESRDG MTARAQELEW ETLQRLQSRI AGTIDEVSRV VYDVTHKPPA TIEYE

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20037.1.
PIR	A84334.
RefSeq	NP_280557.1.
3D structure databases
SMR	Q9HP33. Positions 2-305.
ModBase	Search...
Genome annotation databases
GeneID	1448338.
GenomeReviews	Gene locus VNG_1829G in contig AE004437_GR.
KEGG	hal:VNG1829G.
NMPDR	fig|64091.1.peg.1580.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG391775.
OMA	GIKSHHN.
Enzyme and pathway databases
BioCyc	HSP64091:VNG1829G-MONOMER.
BRENDA	6.3.5.2. 141893.
Family and domain databases
HAMAP	MF_00345. GMP_synthase_B. [Tree]
InterPro	IPR001674. GMP_synth_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00958. GMP_synt_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. guaA_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUAAB_HALSA

Accession

Primary (citable) accession number: Q9HP33

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)