Reviewed, UniProtKB/Swiss-Prot Q9HNZ0 (NADB_HALSA)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	VNG_1883G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 509	509	L-aspartate oxidase		PRO_0000184407
Regions
	Nucleotide binding	10 – 24	15	FAD Potential
Sites
	Active site	235	1	By similarity
	Active site	252	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HNZ0-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2932FA3AB128F10E	FASTA	509	53,226
	10 20 30 40 50 60 MTDATTTDVL VLGSGIAGCG AALAAAREGA SVLVATKAQQ PADASTDWAQ GGIATTRDDP 70 80 90 100 110 120 ESLKRDILAA GDGEADPEAV DALVGDAAAA VEDVLVDTLG VPFDGEEGFD YAREAAHSAA 130 140 150 160 170 180 RILHVDAATG HHILGPFLRH LDAHENVDML EDAAALDLIT DEGAVTGALL DRNPRTGDRA 190 200 210 220 230 240 ETGVPVFAGS TVLATGGIGD LYRRSTNPRG STGDGVAMAA LAGADVTDAE YVQFHPTAYD 250 260 270 280 290 300 DADPFLVSEA VRGEGALLRN ADGERFMPDY HEDAELAPRD VVARAVAAER DATGEVRLDV 310 320 330 340 350 360 SPLAFAEEFP GLAEACADRG VDWETGIPVA PCEHFLCGGV AVDTVGRTSL DRLFAVGECA 370 380 390 400 410 420 RTGVHGANRL ASTSLLEGLV WGLRAGETAA GDDRAPAPSE PPELRDRDPD LPDGFAAEKF 430 440 450 460 470 480 RRLRRVMDEH VGLRRTGADL QRAQGVLRRL KGEVDSYART RTSRDLYQLR NAAVVGLLIA 490 500 RAAGENPESA GCHHRSDEAA AEEAPDAGH

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004437 Genomic DNA. Translation: AAG20080.1.
PIR	D84339.
RefSeq	NP_280600.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1448381.
GenomeReviews	Gene locus VNG_1883G in contig AE004437_GR.
KEGG	hal:VNG1883G.
NMPDR	fig|64091.1.peg.1623.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HNZ0.
OMA	Q9HNZ0. GAYIWNR.
Enzyme and pathway databases
BioCyc	HSP64091:VNG1883G-MON.
BRENDA	1.4.3.16. 141893.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProtoNet	Search...

Entry information

Entry name

NADB_HALSA

Accession

Primary (citable) accession number: Q9HNZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)