Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene

purQ

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851NucleophileUniRule annotation
Active sitei202 – 2021UniRule annotation
Active sitei204 – 2041UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutaminase activity Source: UniProtKB-EC
  3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-HAMAP
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
Short name:
FGAM synthaseUniRule annotation
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit IUniRule annotation
Short name:
FGAR amidotransferase IUniRule annotation
Short name:
FGAR-AT IUniRule annotation
Glutaminase PurQUniRule annotation (EC:3.5.1.2UniRule annotation)
Phosphoribosylformylglycinamidine synthase subunit IUniRule annotation
Gene namesi
Name:purQUniRule annotation
Ordered Locus Names:VNG_1945G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Phosphoribosylformylglycinamidine synthase subunit PurQPRO_0000100606Add
BLAST

Proteomic databases

PaxDbiQ9HNU2.

Interactioni

Subunit structurei

Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG1945G.

Structurei

3D structure databases

ProteinModelPortaliQ9HNU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 224223Glutamine amidotransferase type-1UniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0047.
InParanoidiQ9HNU2.
KOiK01952.
OMAiNPNGSQR.
PhylomeDBiQ9HNU2.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00421. PurQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_PurQ.
[Graphical view]
PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HNU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVAVVRFGG SNCDRDAVRA LAHLGVDAAV AWHDDGLPAD TDGVVVPGGF
60 70 80 90 100
SYGDYLRAGA MAAQSPVVAE VRALAADGVP VLGVCNGAQI GCEAGLAPGA
110 120 130 140 150
FTTNASARFQ CERVHLRVEN ATTPWTAAYS EGDVLEIPIA HGEGRFEIDD
160 170 180 190 200
DAYADLVADD RVLFRYCNAD GEVTEAANPN GSTGAVAGVT GDRDHVAVMM
210 220
PHPERATLPA LGATDGQGIL GAFA
Length:224
Mass (Da):22,980
Last modified:March 1, 2001 - v1
Checksum:iE13165BD3136330A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20128.1.
PIRiD84345.
RefSeqiNP_280648.1. NC_002607.1.
WP_010903428.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20128; AAG20128; VNG_1945G.
GeneIDi1448430.
KEGGihal:VNG1945G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20128.1.
PIRiD84345.
RefSeqiNP_280648.1. NC_002607.1.
WP_010903428.1. NC_002607.1.

3D structure databases

ProteinModelPortaliQ9HNU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64091.VNG1945G.

Proteomic databases

PaxDbiQ9HNU2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG20128; AAG20128; VNG_1945G.
GeneIDi1448430.
KEGGihal:VNG1945G.

Phylogenomic databases

eggNOGiCOG0047.
InParanoidiQ9HNU2.
KOiK01952.
OMAiNPNGSQR.
PhylomeDBiQ9HNU2.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00421. PurQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_PurQ.
[Graphical view]
PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiPURQ_HALSA
AccessioniPrimary (citable) accession number: Q9HNU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: April 29, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.