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Q9HNU2

- PURQ_HALSA

UniProt

Q9HNU2 - PURQ_HALSA

Protein

Phosphoribosylformylglycinamidine synthase subunit PurQ

Gene

purQ

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.UniRule annotation

    Catalytic activityi

    ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation
    L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei85 – 851NucleophileUniRule annotation
    Active sitei202 – 2021UniRule annotation
    Active sitei204 – 2041UniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutaminase activity Source: UniProtKB-EC
    3. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation (EC:6.3.5.3UniRule annotation)
    Short name:
    FGAM synthaseUniRule annotation
    Alternative name(s):
    Formylglycinamide ribonucleotide amidotransferase subunit IUniRule annotation
    Short name:
    FGAR amidotransferase IUniRule annotation
    Short name:
    FGAR-AT IUniRule annotation
    Glutaminase PurQUniRule annotation (EC:3.5.1.2UniRule annotation)
    Phosphoribosylformylglycinamidine synthase subunit IUniRule annotation
    Gene namesi
    Name:purQUniRule annotation
    Ordered Locus Names:VNG_1945G
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 224224Phosphoribosylformylglycinamidine synthase subunit PurQPRO_0000100606Add
    BLAST

    Proteomic databases

    PaxDbiQ9HNU2.

    Interactioni

    Subunit structurei

    Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.UniRule annotation

    Protein-protein interaction databases

    STRINGi64091.VNG1945G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HNU2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 224223Glutamine amidotransferase type-1UniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-1 domain.UniRule annotation

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0047.
    KOiK01952.
    OMAiFVCKWVK.
    PhylomeDBiQ9HNU2.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    HAMAPiMF_00421. PurQ.
    InterProiIPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010075. PRibForGlyAmidine_synth_I.
    [Graphical view]
    PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
    SUPFAMiSSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HNU2-1 [UniParc]FASTAAdd to Basket

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    MTVAVVRFGG SNCDRDAVRA LAHLGVDAAV AWHDDGLPAD TDGVVVPGGF    50
    SYGDYLRAGA MAAQSPVVAE VRALAADGVP VLGVCNGAQI GCEAGLAPGA 100
    FTTNASARFQ CERVHLRVEN ATTPWTAAYS EGDVLEIPIA HGEGRFEIDD 150
    DAYADLVADD RVLFRYCNAD GEVTEAANPN GSTGAVAGVT GDRDHVAVMM 200
    PHPERATLPA LGATDGQGIL GAFA 224
    Length:224
    Mass (Da):22,980
    Last modified:March 1, 2001 - v1
    Checksum:iE13165BD3136330A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20128.1.
    PIRiD84345.
    RefSeqiNP_280648.1. NC_002607.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20128; AAG20128; VNG_1945G.
    GeneIDi1448430.
    KEGGihal:VNG1945G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20128.1 .
    PIRi D84345.
    RefSeqi NP_280648.1. NC_002607.1.

    3D structure databases

    ProteinModelPortali Q9HNU2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG1945G.

    Proteomic databases

    PaxDbi Q9HNU2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG20128 ; AAG20128 ; VNG_1945G .
    GeneIDi 1448430.
    KEGGi hal:VNG1945G.

    Phylogenomic databases

    eggNOGi COG0047.
    KOi K01952.
    OMAi FVCKWVK.
    PhylomeDBi Q9HNU2.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00128 .

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    HAMAPi MF_00421. PurQ.
    InterProi IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR010075. PRibForGlyAmidine_synth_I.
    [Graphical view ]
    PIRSFi PIRSF001586. FGAM_synth_I. 1 hit.
    SUPFAMi SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR01737. FGAM_synth_I. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.

    Entry informationi

    Entry nameiPURQ_HALSA
    AccessioniPrimary (citable) accession number: Q9HNU2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3