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Q9HNU2

- PURQ_HALSA

UniProt

Q9HNU2 - PURQ_HALSA

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Protein
Phosphoribosylformylglycinamidine synthase subunit PurQ
Gene
purQ, VNG_1945G
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL By similarity.

Catalytic activityi

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Nucleophile By similarity
Active sitei202 – 2021 By similarity
Active sitei204 – 2041 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylformylglycinamidine synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00128.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylformylglycinamidine synthase subunit PurQ (EC:6.3.5.3)
Short name:
FGAM synthase
Alternative name(s):
Formylglycinamide ribonucleotide amidotransferase subunit I
Short name:
FGAR amidotransferase I
Short name:
FGAR-AT I
Glutaminase PurQ (EC:3.5.1.2)
Phosphoribosylformylglycinamidine synthase subunit I
Gene namesi
Name:purQ
Ordered Locus Names:VNG_1945G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 224224Phosphoribosylformylglycinamidine synthase subunit PurQUniRule annotation
PRO_0000100606Add
BLAST

Proteomic databases

PaxDbiQ9HNU2.

Interactioni

Subunit structurei

Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits By similarity.

Protein-protein interaction databases

STRINGi64091.VNG1945G.

Structurei

3D structure databases

ProteinModelPortaliQ9HNU2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 224223Glutamine amidotransferase type-1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0047.
KOiK01952.
OMAiFVCKWVK.
PhylomeDBiQ9HNU2.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
HAMAPiMF_00421. PurQ.
InterProiIPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_I.
[Graphical view]
PIRSFiPIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMiSSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01737. FGAM_synth_I. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HNU2-1 [UniParc]FASTAAdd to Basket

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MTVAVVRFGG SNCDRDAVRA LAHLGVDAAV AWHDDGLPAD TDGVVVPGGF    50
SYGDYLRAGA MAAQSPVVAE VRALAADGVP VLGVCNGAQI GCEAGLAPGA 100
FTTNASARFQ CERVHLRVEN ATTPWTAAYS EGDVLEIPIA HGEGRFEIDD 150
DAYADLVADD RVLFRYCNAD GEVTEAANPN GSTGAVAGVT GDRDHVAVMM 200
PHPERATLPA LGATDGQGIL GAFA 224
Length:224
Mass (Da):22,980
Last modified:March 1, 2001 - v1
Checksum:iE13165BD3136330A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20128.1.
PIRiD84345.
RefSeqiNP_280648.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20128; AAG20128; VNG_1945G.
GeneIDi1448430.
KEGGihal:VNG1945G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20128.1 .
PIRi D84345.
RefSeqi NP_280648.1. NC_002607.1.

3D structure databases

ProteinModelPortali Q9HNU2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 64091.VNG1945G.

Proteomic databases

PaxDbi Q9HNU2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20128 ; AAG20128 ; VNG_1945G .
GeneIDi 1448430.
KEGGi hal:VNG1945G.

Phylogenomic databases

eggNOGi COG0047.
KOi K01952.
OMAi FVCKWVK.
PhylomeDBi Q9HNU2.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00128 .

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
HAMAPi MF_00421. PurQ.
InterProi IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR010075. PRibForGlyAmidine_synth_I.
[Graphical view ]
PIRSFi PIRSF001586. FGAM_synth_I. 1 hit.
SUPFAMi SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR01737. FGAM_synth_I. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiPURQ_HALSA
AccessioniPrimary (citable) accession number: Q9HNU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In Gram-negative bacteria and most eukaryotes, FGAM synthase is only formed by PurL, a single polypeptide of 140 kDa (large PurL). However in Gram-positive bacteria and archaebacteria, phosphoribosylformylglycinamidine synthase is composed of three separate proteins: PurL (small PurL), PurQ and PurS By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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