Q9HNU2 (PURQ_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 1
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase I
Short name=FGAM synthase I

Gene names

Name:	purQ
Ordered Locus Names:	VNG_1945G

Organism

Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00421
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00421
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Phosphoribosylformylglycinamidine synthase 1 HAMAP-Rule MF_00421		PRO_0000100606
Regions
	Domain	2 – 224	223	Glutamine amidotransferase type-1
Sites
	Active site	85	1	Nucleophile By similarity
	Active site	202	1	By similarity
	Active site	204	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HNU2 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E13165BD3136330A	FASTA	224	22,980
	10 20 30 40 50 60 MTVAVVRFGG SNCDRDAVRA LAHLGVDAAV AWHDDGLPAD TDGVVVPGGF SYGDYLRAGA 70 80 90 100 110 120 MAAQSPVVAE VRALAADGVP VLGVCNGAQI GCEAGLAPGA FTTNASARFQ CERVHLRVEN 130 140 150 160 170 180 ATTPWTAAYS EGDVLEIPIA HGEGRFEIDD DAYADLVADD RVLFRYCNAD GEVTEAANPN 190 200 210 220 GSTGAVAGVT GDRDHVAVMM PHPERATLPA LGATDGQGIL GAFA

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20128.1.
PIR	D84345.
RefSeq	NP_280648.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HNU2.
ModBase	Search...
Protein-protein interaction databases
STRING	64091.VNG1945G.
Proteomic databases
PaxDb	Q9HNU2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG20128; AAG20128; VNG_1945G.
GeneID	1448430.
KEGG	hal:VNG1945G.
Phylogenomic databases
eggNOG	COG0047.
KO	K01952.
OMA	FPGTNCD.
ProtClustDB	PRK03619.
Enzyme and pathway databases
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00421. PurQ.
InterPro	IPR017926. GATASE_1. IPR010075. PRibForGlyAmidine_synth_I. [Graphical view]
PIRSF	PIRSF001586. FGAM_synth_I. 1 hit.
TIGRFAMs	TIGR01737. FGAM_synth_I. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURQ_HALSA

Accession

Primary (citable) accession number: Q9HNU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2001
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program