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Q9HNL6

- KAE1B_HALSA

UniProt

Q9HNL6 - KAE1B_HALSA

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Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene
VNG_2045G
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function By similarity.UniRule annotation

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.UniRule annotation
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

Binds 1 Fe2+ ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi107 – 1071Iron By similarity
Metal bindingi111 – 1111Iron By similarity
Binding sitei160 – 1601Threonylcarbamoyl-AMP By similarity
Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogen By similarity
Binding sitei177 – 1771Threonylcarbamoyl-AMP By similarity
Binding sitei256 – 2561Threonylcarbamoyl-AMP By similarity
Metal bindingi284 – 2841Iron By similarity
Binding sitei355 – 3551ATP By similarity
Active sitei444 – 4441Proton acceptor; for kinase activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi338 – 3469ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. metalloendopeptidase activity Source: InterPro
  4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
  5. protein serine/threonine kinase activity Source: UniProtKB-KW
  6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
  7. zinc ion binding Source: InterPro

GO - Biological processi

  1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Iron, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein
Including the following 2 domains:
tRNA N6-adenosine threonylcarbamoyltransferase (EC:2.6.99.4)
Alternative name(s):
N6-L-threonylcarbamoyladenine synthase
Short name:
t(6)A synthase
t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1
tRNA threonylcarbamoyladenosine biosynthesis protein Kae1
Serine/threonine-protein kinase Bud32 (EC:2.7.11.1)
Gene namesi
Ordered Locus Names:VNG_2045G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 532532Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotationPRO_0000096978Add
BLAST

Proteomic databases

PaxDbiQ9HNL6.

Interactioni

Subunit structurei

Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes By similarity.

Protein-protein interaction databases

STRINGi64091.VNG2045G.

Structurei

3D structure databases

ProteinModelPortaliQ9HNL6.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini329 – 532204Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 323323Kae1UniRule annotationAdd
BLAST
Regioni128 – 1325Threonylcarbamoyl-AMP binding By similarity

Sequence similaritiesi

In the N-terminal section; belongs to the KAE1 / TsaD family.
In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Phylogenomic databases

eggNOGiCOG0533.
KOiK15904.
PhylomeDBiQ9HNL6.

Family and domain databases

HAMAPiMF_01447. Kae1_Bud32_arch.
InterProiIPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSiPR00789. OSIALOPTASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HNL6-1 [UniParc]FASTAAdd to Basket

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MRVLGVEGTA WCASAALYDA DAAADSVVIE SDAYQPDSGG IHPREAAEHM    50
REAIPAVIET VLGAADGDID AVAFSRGPGL GPCLRIVGSA ARALAQALDV 100
PLVGVNHMVA HLEIGRHQSG FQQPVCLNAS GANAHVLAYR NGRYRVLGET 150
MDTGVGNAID KFTRHVGWQH PGGPKVETHA RDGEYTALPY VVKGMDFSFS 200
GIMSAAKDAV DDGVPVADVC RGLEETMFAM LTEVAERALA LTGRDELVLG 250
GGVGQNDRLR GMLEAMCAAR GASFHAPEPR FLRDNAGMIA VLGAKMAAAG 300
ATIPVADSAI NSQFRPDEVS VTWRDPESPA RDPGADAVRQ GAEATVTFAD 350
DAVIKERAPK AYRHDRLDDR LRRDRTVLEA RLTSDARRQG VPTPLVRDVD 400
VPAATITLQH VGDADLRDAL SPARVRAVGR HLATIHDGGF VHGDPTTRNV 450
RVGAERTFLI DFGLGYYTDA VEDYAMDCHV FEQSLVGTAA DADALVAAFE 500
DAYEEAAASG RVLDQLRAVE GRGRYQDDPE TA 532
Length:532
Mass (Da):56,592
Last modified:June 21, 2005 - v2
Checksum:iE7A1841C2CCC2DC5
GO

Sequence cautioni

The sequence AAG20204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20204.1. Different initiation.
PIRiH84354.
RefSeqiNP_280724.2. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20204; AAG20204; VNG_2045G.
GeneIDi1448508.
KEGGihal:VNG2045G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20204.1 . Different initiation.
PIRi H84354.
RefSeqi NP_280724.2. NC_002607.1.

3D structure databases

ProteinModelPortali Q9HNL6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 64091.VNG2045G.

Proteomic databases

PaxDbi Q9HNL6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20204 ; AAG20204 ; VNG_2045G .
GeneIDi 1448508.
KEGGi hal:VNG2045G.

Phylogenomic databases

eggNOGi COG0533.
KOi K15904.
PhylomeDBi Q9HNL6.

Family and domain databases

HAMAPi MF_01447. Kae1_Bud32_arch.
InterProi IPR022495. Bud32.
IPR000905. Gcp-like_dom.
IPR022449. Kae1.
IPR017861. KAE1/YgjD.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view ]
Pfami PF00814. Peptidase_M22. 1 hit.
[Graphical view ]
PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
PRINTSi PR00789. OSIALOPTASE.
SUPFAMi SSF56112. SSF56112. 1 hit.
TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiKAE1B_HALSA
AccessioniPrimary (citable) accession number: Q9HNL6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: June 11, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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