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Q9HNL6 (KAE1B_HALSA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Including the following 2 domains:

  1. Probable tRNA threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
    Alternative name(s):
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1 homolog
  2. Probable serine/threonine-protein kinase BUD32 homolog
    EC=2.7.11.1
Gene names
Ordered Locus Names:VNG_2045G
OrganismHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]
Taxonomic identifier64091 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium ›

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP-Rule MF_01447

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. HAMAP-Rule MF_01447

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01447.

Sequence similarities

In the N-terminal section; belongs to the KAE1 / YgjD family.

In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAG20204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 532532Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein HAMAP-Rule MF_01447
PRO_0000096978

Regions

Domain329 – 532204Protein kinase
Nucleotide binding338 – 3469ATP By similarity
Region1 – 323323Kae1 HAMAP-Rule MF_01447

Sites

Active site4441Proton acceptor By similarity
Binding site3551ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HNL6 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: E7A1841C2CCC2DC5

FASTA53256,592
        10         20         30         40         50         60 
MRVLGVEGTA WCASAALYDA DAAADSVVIE SDAYQPDSGG IHPREAAEHM REAIPAVIET 

        70         80         90        100        110        120 
VLGAADGDID AVAFSRGPGL GPCLRIVGSA ARALAQALDV PLVGVNHMVA HLEIGRHQSG 

       130        140        150        160        170        180 
FQQPVCLNAS GANAHVLAYR NGRYRVLGET MDTGVGNAID KFTRHVGWQH PGGPKVETHA 

       190        200        210        220        230        240 
RDGEYTALPY VVKGMDFSFS GIMSAAKDAV DDGVPVADVC RGLEETMFAM LTEVAERALA 

       250        260        270        280        290        300 
LTGRDELVLG GGVGQNDRLR GMLEAMCAAR GASFHAPEPR FLRDNAGMIA VLGAKMAAAG 

       310        320        330        340        350        360 
ATIPVADSAI NSQFRPDEVS VTWRDPESPA RDPGADAVRQ GAEATVTFAD DAVIKERAPK 

       370        380        390        400        410        420 
AYRHDRLDDR LRRDRTVLEA RLTSDARRQG VPTPLVRDVD VPAATITLQH VGDADLRDAL 

       430        440        450        460        470        480 
SPARVRAVGR HLATIHDGGF VHGDPTTRNV RVGAERTFLI DFGLGYYTDA VEDYAMDCHV 

       490        500        510        520        530 
FEQSLVGTAA DADALVAAFE DAYEEAAASG RVLDQLRAVE GRGRYQDDPE TA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004437 Genomic DNA. Translation: AAG20204.1. Different initiation.
PIRH84354.
RefSeqNP_280724.2. NC_002607.1.

3D structure databases

ProteinModelPortalQ9HNL6.
ModBaseSearch...

Protein-protein interaction databases

STRING64091.VNG2045G.

Proteomic databases

PaxDbQ9HNL6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG20204; AAG20204; VNG_2045G.
GeneID1448508.
KEGGhal:VNG2045G.

Phylogenomic databases

eggNOGCOG0533.
KOK15904.
ProtClustDBPRK09605.

Family and domain databases

HAMAPMF_01447. Kae1_Bud32_arch.
InterProIPR000905. Gcp-like_dom.
IPR022495. Kae1-assoc_kinase_Bud32.
IPR017861. KAE1/YgjD.
IPR022449. Kae1_arc.
IPR011009. Kinase-like_dom.
IPR017860. Peptidase_M22_CS.
IPR009220. tRNA_threonyl_synthase/kinase.
[Graphical view]
PANTHERPTHR11735:SF6. PTHR11735:SF6. 1 hit.
PfamPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PIRSFPIRSF036401. Gcp_STYKS. 1 hit.
PRINTSPR00789. OSIALOPTASE.
SUPFAMSSF56112. Kinase_like. 1 hit.
TIGRFAMsTIGR03724. arch_bud32. 1 hit.
TIGR03722. arch_KAE1. 1 hit.
TIGR00329. gcp_kae1. 1 hit.
PROSITEPS01016. GLYCOPROTEASE. 1 hit.
PS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. False negative.
PS00109. PROTEIN_KINASE_TYR. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKAE1B_HALSA
AccessionPrimary (citable) accession number: Q9HNL6
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: May 1, 2013
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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