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Q9HNL6

- KAE1B_HALSA

UniProt

Q9HNL6 - KAE1B_HALSA

Protein

Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein

Gene

VNG_2045G

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.UniRule annotation

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.UniRule annotation
    L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi107 – 1071IronUniRule annotation
    Metal bindingi111 – 1111IronUniRule annotation
    Binding sitei160 – 1601Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei173 – 1731Threonylcarbamoyl-AMP; via amide nitrogenUniRule annotation
    Binding sitei177 – 1771Threonylcarbamoyl-AMPUniRule annotation
    Binding sitei256 – 2561Threonylcarbamoyl-AMPUniRule annotation
    Metal bindingi284 – 2841IronUniRule annotation
    Binding sitei355 – 3551ATPUniRule annotation
    Active sitei444 – 4441Proton acceptor; for kinase activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi338 – 3469ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. metalloendopeptidase activity Source: InterPro
    4. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-HAMAP
    5. protein serine/threonine kinase activity Source: UniProtKB-KW
    6. transferase activity, transferring acyl groups other than amino-acyl groups Source: UniProtKB-HAMAP
    7. zinc ion binding Source: InterPro

    GO - Biological processi

    1. threonylcarbamoyladenosine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Acyltransferase, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Iron, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinUniRule annotation
    Including the following 2 domains:
    tRNA N6-adenosine threonylcarbamoyltransferaseUniRule annotation (EC:2.6.99.4UniRule annotation)
    Alternative name(s):
    N6-L-threonylcarbamoyladenine synthase
    Short name:
    t(6)A synthase
    t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    tRNA threonylcarbamoyladenosine biosynthesis protein Kae1UniRule annotation
    Serine/threonine-protein kinase Bud32UniRule annotation (EC:2.7.11.1UniRule annotation)
    Gene namesi
    Ordered Locus Names:VNG_2045G
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 532532Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis proteinPRO_0000096978Add
    BLAST

    Proteomic databases

    PaxDbiQ9HNL6.

    Interactioni

    Subunit structurei

    Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.UniRule annotation

    Protein-protein interaction databases

    STRINGi64091.VNG2045G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HNL6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini329 – 532204Protein kinaseUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 323323Kae1Add
    BLAST
    Regioni128 – 1325Threonylcarbamoyl-AMP bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the KAE1 / TsaD family.UniRule annotation
    In the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.UniRule annotation
    Contains 1 protein kinase domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0533.
    KOiK15904.
    PhylomeDBiQ9HNL6.

    Family and domain databases

    HAMAPiMF_01447. Kae1_Bud32_arch.
    InterProiIPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR009220. tRNA_threonyl_synthase/kinase.
    [Graphical view]
    PfamiPF00814. Peptidase_M22. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSiPR00789. OSIALOPTASE.
    SUPFAMiSSF56112. SSF56112. 1 hit.
    TIGRFAMsiTIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEiPS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9HNL6-1 [UniParc]FASTAAdd to Basket

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    MRVLGVEGTA WCASAALYDA DAAADSVVIE SDAYQPDSGG IHPREAAEHM    50
    REAIPAVIET VLGAADGDID AVAFSRGPGL GPCLRIVGSA ARALAQALDV 100
    PLVGVNHMVA HLEIGRHQSG FQQPVCLNAS GANAHVLAYR NGRYRVLGET 150
    MDTGVGNAID KFTRHVGWQH PGGPKVETHA RDGEYTALPY VVKGMDFSFS 200
    GIMSAAKDAV DDGVPVADVC RGLEETMFAM LTEVAERALA LTGRDELVLG 250
    GGVGQNDRLR GMLEAMCAAR GASFHAPEPR FLRDNAGMIA VLGAKMAAAG 300
    ATIPVADSAI NSQFRPDEVS VTWRDPESPA RDPGADAVRQ GAEATVTFAD 350
    DAVIKERAPK AYRHDRLDDR LRRDRTVLEA RLTSDARRQG VPTPLVRDVD 400
    VPAATITLQH VGDADLRDAL SPARVRAVGR HLATIHDGGF VHGDPTTRNV 450
    RVGAERTFLI DFGLGYYTDA VEDYAMDCHV FEQSLVGTAA DADALVAAFE 500
    DAYEEAAASG RVLDQLRAVE GRGRYQDDPE TA 532
    Length:532
    Mass (Da):56,592
    Last modified:June 21, 2005 - v2
    Checksum:iE7A1841C2CCC2DC5
    GO

    Sequence cautioni

    The sequence AAG20204.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20204.1. Different initiation.
    PIRiH84354.
    RefSeqiNP_280724.2. NC_002607.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20204; AAG20204; VNG_2045G.
    GeneIDi1448508.
    KEGGihal:VNG2045G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20204.1 . Different initiation.
    PIRi H84354.
    RefSeqi NP_280724.2. NC_002607.1.

    3D structure databases

    ProteinModelPortali Q9HNL6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG2045G.

    Proteomic databases

    PaxDbi Q9HNL6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG20204 ; AAG20204 ; VNG_2045G .
    GeneIDi 1448508.
    KEGGi hal:VNG2045G.

    Phylogenomic databases

    eggNOGi COG0533.
    KOi K15904.
    PhylomeDBi Q9HNL6.

    Family and domain databases

    HAMAPi MF_01447. Kae1_Bud32_arch.
    InterProi IPR022495. Bud32.
    IPR000905. Gcp-like_dom.
    IPR022449. Kae1.
    IPR017861. KAE1/YgjD.
    IPR011009. Kinase-like_dom.
    IPR017860. Peptidase_M22_CS.
    IPR009220. tRNA_threonyl_synthase/kinase.
    [Graphical view ]
    Pfami PF00814. Peptidase_M22. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036401. Gcp_STYKS. 1 hit.
    PRINTSi PR00789. OSIALOPTASE.
    SUPFAMi SSF56112. SSF56112. 1 hit.
    TIGRFAMsi TIGR03724. arch_bud32. 1 hit.
    TIGR03722. arch_KAE1. 1 hit.
    TIGR00329. gcp_kae1. 1 hit.
    PROSITEi PS01016. GLYCOPROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.

    Entry informationi

    Entry nameiKAE1B_HALSA
    AccessioniPrimary (citable) accession number: Q9HNL6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 85 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3