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Protein

Glutamine synthetase

Gene

glnA

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Glutamine synthetase (GS) is an unusual multitasking protein that functions as an enzyme, a transcription coregulator, and a chaperone in ammonium assimilation and in the regulation of genes involved in nitrogen metabolism. It catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia. Feedback-inhibited GlnA also interacts with and regulates the activity of the transcriptional regulator TnrA. During nitrogen limitation, TnrA is in its DNA-binding active state and turns on the transcription of genes required for nitrogen assimilation. Under conditions of nitrogen excess, feedback-inhibited GlnA forms a stable complex with TnrA, which inhibits its DNA-binding activity. In contrast, feedback-inhibited GlnA acts as a chaperone to stabilize the DNA-binding activity of GlnR, which represses the transcription of nitrogen assimilation genes.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.By similarity

Cofactori

Mg2+By similarityNote: Binds 2 Mg2+ ions per subunit.By similarity

Enzyme regulationi

Inhibited by glutamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei71Important for inhibition by glutamineBy similarity1
Metal bindingi141Magnesium 1By similarity1
Metal bindingi143Magnesium 2By similarity1
Binding sitei193ATPBy similarity1
Metal bindingi198Magnesium 2By similarity1
Metal bindingi205Magnesium 2By similarity1
Binding sitei250L-glutamate; via carbonyl oxygenBy similarity1
Metal bindingi254Magnesium 1; via pros nitrogenBy similarity1
Binding sitei258ATPBy similarity1
Binding sitei308L-glutamateBy similarity1
Binding sitei314L-glutamateBy similarity1
Binding sitei326ATPBy similarity1
Binding sitei326L-glutamateBy similarity1
Binding sitei331ATPBy similarity1
Metal bindingi343Magnesium 1By similarity1
Binding sitei345L-glutamateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi256 – 258ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetaseBy similarity (EC:6.3.1.2By similarity)
Short name:
GSBy similarity
Alternative name(s):
Glutamate--ammonia ligaseBy similarity
Glutamine synthetase I alphaBy similarity
Short name:
GSI alphaBy similarity
Gene namesi
Name:glnABy similarity
Ordered Locus Names:VNG_2093G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000000554 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001532021 – 454Glutamine synthetaseAdd BLAST454

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9HNI2.
PRIDEiQ9HNI2.

Interactioni

Subunit structurei

Oligomer of 12 subunits arranged in the form of two hexagons. In its feedback-inhibited form, interacts with TnrA in order to block its DNA-binding activity.By similarity

Protein-protein interaction databases

STRINGi64091.VNG2093G.

Structurei

3D structure databases

ProteinModelPortaliQ9HNI2.
SMRiQ9HNI2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 250L-glutamate bindingBy similarity2

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
InParanoidiQ9HNI2.
KOiK01915.
OMAiMGFTCYT.
OrthoDBiPOG093Z00GE.
PhylomeDBiQ9HNI2.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HNI2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNGDSSSSA LTDNERAVLD DIEAQGIDFL RLQFTDILGT VKNVSIPAHQ
60 70 80 90 100
AEKAFTEGIY FDGSSIEGFV RIQESDMRLD PDPETFAVLP WRSNGDGGSA
110 120 130 140 150
RLICDVVDRE GNAFAGGPRQ VLKNVLARAD DMGYSVSIGP EPEFFLFEKD
160 170 180 190 200
DDGNATTTAH DQGGYFDLAP KDLASDIRRE IIFTLEAMGF EIEASHHEVA
210 220 230 240 250
RGQHEINFKY DDALTTADNI ATFRAVVRAV AEQHDVHATF MPKPIGEING
260 270 280 290 300
SGMHSHISLF DEDGENVFAD NDDEFNLSET AYQFMGGVLE HAPAFTAVTN
310 320 330 340 350
PTVNSYKRLV PGYEAPVYIA WSGVNRSALI RVPDAAGVSA RFEIRSPDPS
360 370 380 390 400
CNPYLALAAV IAAGLDGIDT DADPGDAVRE DIYEFDEDKR DAYGIDTLPG
410 420 430 440 450
HLGDAVTALE SDPVMQDALG EHVCEKFAEA KRHEYAEYKA SVSEWETDRY

LEKF
Length:454
Mass (Da):49,757
Last modified:March 1, 2001 - v1
Checksum:i09C12E8D0B91D538
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20238.1.
PIRiB84359.
RefSeqiWP_010903540.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20238; AAG20238; VNG_2093G.
GeneIDi5953234.
KEGGihal:VNG_2093G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20238.1.
PIRiB84359.
RefSeqiWP_010903540.1. NC_002607.1.

3D structure databases

ProteinModelPortaliQ9HNI2.
SMRiQ9HNI2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64091.VNG2093G.

Proteomic databases

PaxDbiQ9HNI2.
PRIDEiQ9HNI2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG20238; AAG20238; VNG_2093G.
GeneIDi5953234.
KEGGihal:VNG_2093G.

Phylogenomic databases

eggNOGiarCOG01909. Archaea.
COG0174. LUCA.
InParanoidiQ9HNI2.
KOiK01915.
OMAiMGFTCYT.
OrthoDBiPOG093Z00GE.
PhylomeDBiQ9HNI2.

Family and domain databases

Gene3Di3.10.20.70. 1 hit.
3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR004809. Gln_synth_I.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
TIGRFAMsiTIGR00653. GlnA. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLN1A_HALSA
AccessioniPrimary (citable) accession number: Q9HNI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: March 1, 2001
Last modified: March 15, 2017
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.