Q9HNF4 (RIFK_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin kinase
Short name=RFK
EC=2.7.1.161

Alternative name(s):
CTP-dependent riboflavin kinase
CTP:riboflavin 5'-phosphotransferase
Flavokinase

Gene names

Name:	ribK
Ordered Locus Names:	VNG_2126C

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN) By similarity. HAMAP MF_01285
Catalytic activity	CTP + riboflavin = CDP + FMN. HAMAP MF_01285
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_01285
Pathway	Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1. HAMAP MF_01285
Sequence similarities	Belongs to the archaeal riboflavin kinase family.

Ontologies

Keywords
Ligand	FMN Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW riboflavin kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Riboflavin kinase HAMAP MF_01285		PRO_0000322085
Regions
	Nucleotide binding	118 – 123	6	CDP By similarity
	Nucleotide binding	222 – 225	4	CDP By similarity
	Region	1 – 108	108	Unknown HAMAP MF_01285
	Region	109 – 242	134	Riboflavin kinase HAMAP MF_01285
Sites
	Metal binding	147	1	Magnesium By similarity
	Metal binding	149	1	Magnesium By similarity
	Binding site	209	1	FMN By similarity
	Binding site	217	1	FMN By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HNF4 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 7FB9D5E0F7646CD2	FASTA	242	26,222
	10 20 30 40 50 60 MRGAGQPPAM SGATSTGDVG YDELAVLKLL ALDGAHRGEV KVSCGDLASR LDASSQTASR 70 80 90 100 110 120 RLQALDDADH VTRDLVSDGQ WITVTDAGRH ALKHEYEDYR RIFEDPGELA LAGTVTSGMG 130 140 150 160 170 180 EGRHYISLPG YNRQFAEKLG YEPYPGTLNV DLPPDGQRAR AGIQALDGVD IDAWEDEDRT 190 200 210 220 230 240 YGSATCYACT VVGDGTDFDG AHVIIPDRTH HDDDQLEIIA PVKLRERLGL LDDDEVTIRV EA

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20266.1.
PIR	F84362.
RefSeq	NP_280786.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HNF4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1448570.
GenomeReviews	Gene locus VNG_2126C in contig AE004437_GR.
KEGG	hal:VNG2126C.
NMPDR	fig|64091.1.peg.1812.
Phylogenomic databases
HOGENOM	HBG553349.
OMA	GRHYISL.
ProtClustDB	CLSK511620.
Enzyme and pathway databases
BioCyc	HSP64091:VNG2126C-MONOMER.
Family and domain databases
HAMAP	MF_01285. Riboflavin_kinase. Fused. [Tree]
InterPro	IPR023602. Riboflavin_kinase_CTP-dep. IPR023465. Riboflavin_kinase_domain. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.40.30.30. Riboflavin_kinase. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K07732.
Pfam	PF01982. CTP-dep_RFKase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIFK_HALSA

Accession

Primary (citable) accession number: Q9HNF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	March 1, 2001
Last modified:	November 16, 2011
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program