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Q9HN97

- SYI_HALSA

UniProt

Q9HN97 - SYI_HALSA

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Protein
Isoleucine--tRNA ligase
Gene
ileS, VNG_2190G
Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei609 – 6091ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:VNG_2190G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10701070Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098577Add
BLAST

Proteomic databases

PaxDbiQ9HN97.
PRIDEiQ9HN97.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG2190G.

Structurei

3D structure databases

ProteinModelPortaliQ9HN97.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotation
Add
BLAST
Motifi606 – 6105"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiLGHRVHY.
PhylomeDBiQ9HN97.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HN97-1 [UniParc]FASTAAdd to Basket

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MSGFGDVPDQ YDPAGVEERV FSYWEAVDAY EQTVDHRADA ETFFFVDGPP     50
YTSGAAHMGT TWNKTLKDAY IRYHRMQGYD VTDRPGYDMH GLPIETKVEE 100
QLGFESKKDI QEFGEEAFIE ECKRFADDNL DGLQSDFQSF GVWMDWDNPY 150
KTVDPSYMEA AWWAFSEVHD NGLVERGQRS ISQCPRCETA IANNEVEYED 200
VTDPSIYVRF DLDDREGSLV VWTTTPWTIP ANQFVAVDED GDYQQVRATT 250
PDGETDVLYV ASECVEDVLS AGGYSDHEIV ADHSGSDLIG WSYTPPLADA 300
VPANPTDADG THEVYHGDWV EADRTGLVHS APGHGEEDFE RGAELDLPVF 350
CPVGEDGVYT DAGGKYAGAF VRDANDDIIA DLEARDAMLA SQTVEHSYGH 400
CWRCDTGIIQ IVTDQWFITI TDVKDELLAN MDTSEWHPEW ARDNRFRDFV 450
ENAPDWNVSR QRYWGIPVPI WTPEDWSGDA EDVLVVGTRE ELAALADQDV 500
DPASVDLHKP TVDDITITED GTEYTRVPDV FDVWLDSSVA SWGTLNYPEQ 550
EDDFDELWPA DLIMEAHDQT RGWFWSQLGM GTAALGDVPY EEVLMHGYAN 600
MPDGRGMSKS KGITIEPNEV IDEYGADPMR MFLLSVTPQG DDMSFSWDET 650
ENMQRDLNIL WNVFRFPRPY MALDDFDANV PAAFGGDDAG VGIADADLET 700
VDEWLLSRLQ HVKADATAHW EDFEQHRALD AVLEFVVEDL SRYYVQVVRE 750
RMWEDDASAS KTAAYATMQR VLLEVVALLA PYAPFVTEEL YSHLTGDRGY 800
DTVHMADWPT VEESLRAPAL EADVAVLRAA EEAGSHARQQ AGRKLRWPVT 850
RVVVDAAEDS VVDALDAHGD LLADRLNTRA IEVVEPGAAW DELAYSARAD 900
MSELGPAFGD DAGAVMNALN DARVTDRDID ALAEQVAADL GRDDIELTTE 950
MVEFVEETPE HVAGAAFETE TAAGTVYVDT ELNEDVESEG YAREVVRRVQ 1000
EMRKEMDLAM DAEIRLDVLV FDERVGELVA RHEPLITAET RARELGEVED 1050
GHREEWDVEG TTMILEVEAV 1070
Length:1,070
Mass (Da):119,902
Last modified:March 1, 2001 - v1
Checksum:iEABADEAC91ECBDC3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20324.1.
PIRiH84369.
RefSeqiNP_280844.1. NC_002607.1.
WP_010903625.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20324; AAG20324; VNG_2190G.
GeneIDi1448627.
KEGGihal:VNG2190G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20324.1 .
PIRi H84369.
RefSeqi NP_280844.1. NC_002607.1.
WP_010903625.1. NC_002607.1.

3D structure databases

ProteinModelPortali Q9HN97.
ModBasei Search...

Protein-protein interaction databases

STRINGi 64091.VNG2190G.

Proteomic databases

PaxDbi Q9HN97.
PRIDEi Q9HN97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20324 ; AAG20324 ; VNG_2190G .
GeneIDi 1448627.
KEGGi hal:VNG2190G.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi LGHRVHY.
PhylomeDBi Q9HN97.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiSYI_HALSA
AccessioniPrimary (citable) accession number: Q9HN97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2001
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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