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Q9HN97

- SYI_HALSA

UniProt

Q9HN97 - SYI_HALSA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei609 – 6091ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VNG_2190G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10701070Isoleucine--tRNA ligasePRO_0000098577Add
BLAST

Proteomic databases

PaxDbiQ9HN97.
PRIDEiQ9HN97.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG2190G.

Structurei

3D structure databases

ProteinModelPortaliQ9HN97.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi606 – 6105"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ9HN97.
KOiK01870.
OMAiLGHRVHY.
PhylomeDBiQ9HN97.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HN97-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGFGDVPDQ YDPAGVEERV FSYWEAVDAY EQTVDHRADA ETFFFVDGPP
60 70 80 90 100
YTSGAAHMGT TWNKTLKDAY IRYHRMQGYD VTDRPGYDMH GLPIETKVEE
110 120 130 140 150
QLGFESKKDI QEFGEEAFIE ECKRFADDNL DGLQSDFQSF GVWMDWDNPY
160 170 180 190 200
KTVDPSYMEA AWWAFSEVHD NGLVERGQRS ISQCPRCETA IANNEVEYED
210 220 230 240 250
VTDPSIYVRF DLDDREGSLV VWTTTPWTIP ANQFVAVDED GDYQQVRATT
260 270 280 290 300
PDGETDVLYV ASECVEDVLS AGGYSDHEIV ADHSGSDLIG WSYTPPLADA
310 320 330 340 350
VPANPTDADG THEVYHGDWV EADRTGLVHS APGHGEEDFE RGAELDLPVF
360 370 380 390 400
CPVGEDGVYT DAGGKYAGAF VRDANDDIIA DLEARDAMLA SQTVEHSYGH
410 420 430 440 450
CWRCDTGIIQ IVTDQWFITI TDVKDELLAN MDTSEWHPEW ARDNRFRDFV
460 470 480 490 500
ENAPDWNVSR QRYWGIPVPI WTPEDWSGDA EDVLVVGTRE ELAALADQDV
510 520 530 540 550
DPASVDLHKP TVDDITITED GTEYTRVPDV FDVWLDSSVA SWGTLNYPEQ
560 570 580 590 600
EDDFDELWPA DLIMEAHDQT RGWFWSQLGM GTAALGDVPY EEVLMHGYAN
610 620 630 640 650
MPDGRGMSKS KGITIEPNEV IDEYGADPMR MFLLSVTPQG DDMSFSWDET
660 670 680 690 700
ENMQRDLNIL WNVFRFPRPY MALDDFDANV PAAFGGDDAG VGIADADLET
710 720 730 740 750
VDEWLLSRLQ HVKADATAHW EDFEQHRALD AVLEFVVEDL SRYYVQVVRE
760 770 780 790 800
RMWEDDASAS KTAAYATMQR VLLEVVALLA PYAPFVTEEL YSHLTGDRGY
810 820 830 840 850
DTVHMADWPT VEESLRAPAL EADVAVLRAA EEAGSHARQQ AGRKLRWPVT
860 870 880 890 900
RVVVDAAEDS VVDALDAHGD LLADRLNTRA IEVVEPGAAW DELAYSARAD
910 920 930 940 950
MSELGPAFGD DAGAVMNALN DARVTDRDID ALAEQVAADL GRDDIELTTE
960 970 980 990 1000
MVEFVEETPE HVAGAAFETE TAAGTVYVDT ELNEDVESEG YAREVVRRVQ
1010 1020 1030 1040 1050
EMRKEMDLAM DAEIRLDVLV FDERVGELVA RHEPLITAET RARELGEVED
1060 1070
GHREEWDVEG TTMILEVEAV
Length:1,070
Mass (Da):119,902
Last modified:March 1, 2001 - v1
Checksum:iEABADEAC91ECBDC3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20324.1.
PIRiH84369.
RefSeqiNP_280844.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20324; AAG20324; VNG_2190G.
GeneIDi1448627.
KEGGihal:VNG2190G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004437 Genomic DNA. Translation: AAG20324.1 .
PIRi H84369.
RefSeqi NP_280844.1. NC_002607.1.

3D structure databases

ProteinModelPortali Q9HN97.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 64091.VNG2190G.

Proteomic databases

PaxDbi Q9HN97.
PRIDEi Q9HN97.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20324 ; AAG20324 ; VNG_2190G .
GeneIDi 1448627.
KEGGi hal:VNG2190G.

Phylogenomic databases

eggNOGi COG0060.
InParanoidi Q9HN97.
KOi K01870.
OMAi LGHRVHY.
PhylomeDBi Q9HN97.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiSYI_HALSA
AccessioniPrimary (citable) accession number: Q9HN97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3