Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei609ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:VNG_2190G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000000554 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985771 – 1070Isoleucine--tRNA ligaseAdd BLAST1070

Proteomic databases

PaxDbiQ9HN97
PRIDEiQ9HN97

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG2190G

Structurei

3D structure databases

ProteinModelPortaliQ9HN97
SMRiQ9HN97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 60"HIGH" regionAdd BLAST11
Motifi606 – 610"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
InParanoidiQ9HN97
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054
PhylomeDBiQ9HN97

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HN97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGFGDVPDQ YDPAGVEERV FSYWEAVDAY EQTVDHRADA ETFFFVDGPP
60 70 80 90 100
YTSGAAHMGT TWNKTLKDAY IRYHRMQGYD VTDRPGYDMH GLPIETKVEE
110 120 130 140 150
QLGFESKKDI QEFGEEAFIE ECKRFADDNL DGLQSDFQSF GVWMDWDNPY
160 170 180 190 200
KTVDPSYMEA AWWAFSEVHD NGLVERGQRS ISQCPRCETA IANNEVEYED
210 220 230 240 250
VTDPSIYVRF DLDDREGSLV VWTTTPWTIP ANQFVAVDED GDYQQVRATT
260 270 280 290 300
PDGETDVLYV ASECVEDVLS AGGYSDHEIV ADHSGSDLIG WSYTPPLADA
310 320 330 340 350
VPANPTDADG THEVYHGDWV EADRTGLVHS APGHGEEDFE RGAELDLPVF
360 370 380 390 400
CPVGEDGVYT DAGGKYAGAF VRDANDDIIA DLEARDAMLA SQTVEHSYGH
410 420 430 440 450
CWRCDTGIIQ IVTDQWFITI TDVKDELLAN MDTSEWHPEW ARDNRFRDFV
460 470 480 490 500
ENAPDWNVSR QRYWGIPVPI WTPEDWSGDA EDVLVVGTRE ELAALADQDV
510 520 530 540 550
DPASVDLHKP TVDDITITED GTEYTRVPDV FDVWLDSSVA SWGTLNYPEQ
560 570 580 590 600
EDDFDELWPA DLIMEAHDQT RGWFWSQLGM GTAALGDVPY EEVLMHGYAN
610 620 630 640 650
MPDGRGMSKS KGITIEPNEV IDEYGADPMR MFLLSVTPQG DDMSFSWDET
660 670 680 690 700
ENMQRDLNIL WNVFRFPRPY MALDDFDANV PAAFGGDDAG VGIADADLET
710 720 730 740 750
VDEWLLSRLQ HVKADATAHW EDFEQHRALD AVLEFVVEDL SRYYVQVVRE
760 770 780 790 800
RMWEDDASAS KTAAYATMQR VLLEVVALLA PYAPFVTEEL YSHLTGDRGY
810 820 830 840 850
DTVHMADWPT VEESLRAPAL EADVAVLRAA EEAGSHARQQ AGRKLRWPVT
860 870 880 890 900
RVVVDAAEDS VVDALDAHGD LLADRLNTRA IEVVEPGAAW DELAYSARAD
910 920 930 940 950
MSELGPAFGD DAGAVMNALN DARVTDRDID ALAEQVAADL GRDDIELTTE
960 970 980 990 1000
MVEFVEETPE HVAGAAFETE TAAGTVYVDT ELNEDVESEG YAREVVRRVQ
1010 1020 1030 1040 1050
EMRKEMDLAM DAEIRLDVLV FDERVGELVA RHEPLITAET RARELGEVED
1060 1070
GHREEWDVEG TTMILEVEAV
Length:1,070
Mass (Da):119,902
Last modified:March 1, 2001 - v1
Checksum:iEABADEAC91ECBDC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA Translation: AAG20324.1
PIRiH84369
RefSeqiWP_010903625.1, NC_002607.1

Genome annotation databases

EnsemblBacteriaiAAG20324; AAG20324; VNG_2190G
GeneIDi5953285
KEGGihal:VNG_2190G
PATRICifig|64091.14.peg.1684

Similar proteinsi

Entry informationi

Entry nameiSYI_HALSA
AccessioniPrimary (citable) accession number: Q9HN97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health