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Q9HN97

- SYI_HALSA

UniProt

Q9HN97 - SYI_HALSA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei609 – 6091ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:VNG_2190G
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10701070Isoleucine--tRNA ligasePRO_0000098577Add
    BLAST

    Proteomic databases

    PaxDbiQ9HN97.
    PRIDEiQ9HN97.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi64091.VNG2190G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HN97.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi606 – 6105"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiLGHRVHY.
    PhylomeDBiQ9HN97.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HN97-1 [UniParc]FASTAAdd to Basket

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    MSGFGDVPDQ YDPAGVEERV FSYWEAVDAY EQTVDHRADA ETFFFVDGPP     50
    YTSGAAHMGT TWNKTLKDAY IRYHRMQGYD VTDRPGYDMH GLPIETKVEE 100
    QLGFESKKDI QEFGEEAFIE ECKRFADDNL DGLQSDFQSF GVWMDWDNPY 150
    KTVDPSYMEA AWWAFSEVHD NGLVERGQRS ISQCPRCETA IANNEVEYED 200
    VTDPSIYVRF DLDDREGSLV VWTTTPWTIP ANQFVAVDED GDYQQVRATT 250
    PDGETDVLYV ASECVEDVLS AGGYSDHEIV ADHSGSDLIG WSYTPPLADA 300
    VPANPTDADG THEVYHGDWV EADRTGLVHS APGHGEEDFE RGAELDLPVF 350
    CPVGEDGVYT DAGGKYAGAF VRDANDDIIA DLEARDAMLA SQTVEHSYGH 400
    CWRCDTGIIQ IVTDQWFITI TDVKDELLAN MDTSEWHPEW ARDNRFRDFV 450
    ENAPDWNVSR QRYWGIPVPI WTPEDWSGDA EDVLVVGTRE ELAALADQDV 500
    DPASVDLHKP TVDDITITED GTEYTRVPDV FDVWLDSSVA SWGTLNYPEQ 550
    EDDFDELWPA DLIMEAHDQT RGWFWSQLGM GTAALGDVPY EEVLMHGYAN 600
    MPDGRGMSKS KGITIEPNEV IDEYGADPMR MFLLSVTPQG DDMSFSWDET 650
    ENMQRDLNIL WNVFRFPRPY MALDDFDANV PAAFGGDDAG VGIADADLET 700
    VDEWLLSRLQ HVKADATAHW EDFEQHRALD AVLEFVVEDL SRYYVQVVRE 750
    RMWEDDASAS KTAAYATMQR VLLEVVALLA PYAPFVTEEL YSHLTGDRGY 800
    DTVHMADWPT VEESLRAPAL EADVAVLRAA EEAGSHARQQ AGRKLRWPVT 850
    RVVVDAAEDS VVDALDAHGD LLADRLNTRA IEVVEPGAAW DELAYSARAD 900
    MSELGPAFGD DAGAVMNALN DARVTDRDID ALAEQVAADL GRDDIELTTE 950
    MVEFVEETPE HVAGAAFETE TAAGTVYVDT ELNEDVESEG YAREVVRRVQ 1000
    EMRKEMDLAM DAEIRLDVLV FDERVGELVA RHEPLITAET RARELGEVED 1050
    GHREEWDVEG TTMILEVEAV 1070
    Length:1,070
    Mass (Da):119,902
    Last modified:March 1, 2001 - v1
    Checksum:iEABADEAC91ECBDC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20324.1.
    PIRiH84369.
    RefSeqiNP_280844.1. NC_002607.1.
    WP_010903625.1. NC_002607.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20324; AAG20324; VNG_2190G.
    GeneIDi1448627.
    KEGGihal:VNG2190G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20324.1 .
    PIRi H84369.
    RefSeqi NP_280844.1. NC_002607.1.
    WP_010903625.1. NC_002607.1.

    3D structure databases

    ProteinModelPortali Q9HN97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG2190G.

    Proteomic databases

    PaxDbi Q9HN97.
    PRIDEi Q9HN97.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG20324 ; AAG20324 ; VNG_2190G .
    GeneIDi 1448627.
    KEGGi hal:VNG2190G.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi LGHRVHY.
    PhylomeDBi Q9HN97.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.

    Entry informationi

    Entry nameiSYI_HALSA
    AccessioniPrimary (citable) accession number: Q9HN97
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3