ID ENDA_HALSA Reviewed; 343 AA. AC Q9HN82; DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot. DT 01-MAR-2001, sequence version 1. DT 27-MAR-2024, entry version 112. DE RecName: Full=tRNA-splicing endonuclease {ECO:0000255|HAMAP-Rule:MF_01834}; DE EC=4.6.1.16 {ECO:0000255|HAMAP-Rule:MF_01834}; DE AltName: Full=tRNA-intron endonuclease {ECO:0000255|HAMAP-Rule:MF_01834}; GN Name=endA {ECO:0000255|HAMAP-Rule:MF_01834}; GN OrderedLocusNames=VNG_2210G; OS Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OS (Halobacterium halobium). OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; OC Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001. OX NCBI_TaxID=64091; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 700922 / JCM 11081 / NRC-1; RX PubMed=11016950; DOI=10.1073/pnas.190337797; RA Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., RA Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., RA Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R., RA Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., RA Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., RA Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., RA Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., RA DasSarma S.; RT "Genome sequence of Halobacterium species NRC-1."; RL Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000). CC -!- FUNCTION: Endonuclease that removes tRNA introns. Cleaves pre-tRNA at CC the 5'- and 3'-splice sites to release the intron. The products are an CC intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and CC 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged CC loops of 3 bases are separated by a stem of 4 bp (By similarity). CC {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'- CC half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with CC a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16; CC Evidence={ECO:0000255|HAMAP-Rule:MF_01834}; CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01834}. CC -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family. Archaeal CC long subfamily. {ECO:0000255|HAMAP-Rule:MF_01834}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; AE004437; AAG20339.1; -; Genomic_DNA. DR PIR; G84371; G84371. DR RefSeq; WP_010903640.1; NC_002607.1. DR AlphaFoldDB; Q9HN82; -. DR SMR; Q9HN82; -. DR STRING; 64091.VNG_2210G; -. DR PaxDb; 64091-VNG_2210G; -. DR GeneID; 68694770; -. DR KEGG; hal:VNG_2210G; -. DR PATRIC; fig|64091.14.peg.1699; -. DR HOGENOM; CLU_791347_0_0_2; -. DR InParanoid; Q9HN82; -. DR OrthoDB; 46045at2157; -. DR PhylomeDB; Q9HN82; -. DR Proteomes; UP000000554; Chromosome. DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW. DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro. DR GO; GO:0000213; F:tRNA-intron endonuclease activity; IBA:GO_Central. DR GO; GO:0000379; P:tRNA-type intron splice site recognition and cleavage; IBA:GO_Central. DR CDD; cd22363; tRNA-intron_lyase_C; 2. DR Gene3D; 3.40.1350.10; -; 2. DR Gene3D; 3.40.1170.20; tRNA intron endonuclease, N-terminal domain; 2. DR HAMAP; MF_01834; EndA_long; 1. DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf. DR InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf. DR InterPro; IPR006677; tRNA_intron_Endonuc_cat-like. DR InterPro; IPR006678; tRNA_intron_Endonuc_N. DR InterPro; IPR036740; tRNA_intron_Endonuc_N_sf. DR InterPro; IPR006676; tRNA_splic. DR InterPro; IPR023516; tRNA_splic_arch_long. DR NCBIfam; TIGR00324; endA; 1. DR PANTHER; PTHR21227; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1. DR PANTHER; PTHR21227:SF0; TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2; 1. DR Pfam; PF01974; tRNA_int_endo; 1. DR Pfam; PF02778; tRNA_int_endo_N; 2. DR SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 2. DR SUPFAM; SSF55267; tRNA-intron endonuclease N-terminal domain-like; 2. PE 3: Inferred from homology; KW Lyase; Reference proteome; tRNA processing. FT CHAIN 1..343 FT /note="tRNA-splicing endonuclease" FT /id="PRO_0000109485" FT ACT_SITE 277 FT /evidence="ECO:0000255|HAMAP-Rule:MF_01834" FT ACT_SITE 288 FT /evidence="ECO:0000255|HAMAP-Rule:MF_01834" FT ACT_SITE 319 FT /evidence="ECO:0000255|HAMAP-Rule:MF_01834" SQ SEQUENCE 343 AA; 37872 MW; E84404AC0C317CA8 CRC64; MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD LDSVDGMGFR AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD GDFVVFPRGN TRRDGVVKHR VRVVDERTTL PVDGLSESVL AVVDEESEIT YLDIAPETPT GETTLDRPTD VPGVLLDDRV LVWEPPQRLH NAGFYGQPLG GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR LGEGEHFDCR LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP //