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Q9HN82

- ENDA_HALSA

UniProt

Q9HN82 - ENDA_HALSA

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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei277 – 2771UniRule annotation
Active sitei288 – 2881UniRule annotation
Active sitei319 – 3191UniRule annotation

GO - Molecular functioni

  1. lyase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:VNG_2210G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343tRNA-splicing endonucleasePRO_0000109485Add
BLAST

Proteomic databases

PaxDbiQ9HN82.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG2210G.

Structurei

3D structure databases

ProteinModelPortaliQ9HN82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1676.
InParanoidiQ9HN82.
KOiK01170.
OMAiRGFYLSP.
PhylomeDBiQ9HN82.

Family and domain databases

Gene3Di3.40.1350.10. 2 hits.
HAMAPiMF_01834. EndA_long.
InterProiIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamiPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMiSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsiTIGR00324. endA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9HN82-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD
60 70 80 90 100
LDSVDGMGFR AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD
110 120 130 140 150
GDFVVFPRGN TRRDGVVKHR VRVVDERTTL PVDGLSESVL AVVDEESEIT
160 170 180 190 200
YLDIAPETPT GETTLDRPTD VPGVLLDDRV LVWEPPQRLH NAGFYGQPLG
210 220 230 240 250
GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR LGEGEHFDCR
260 270 280 290 300
LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH
310 320 330 340
VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP
Length:343
Mass (Da):37,872
Last modified:March 1, 2001 - v1
Checksum:iE84404AC0C317CA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20339.1.
PIRiG84371.
RefSeqiNP_280859.1. NC_002607.1.
WP_010903640.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20339; AAG20339; VNG_2210G.
GeneIDi1448641.
KEGGihal:VNG2210G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20339.1 .
PIRi G84371.
RefSeqi NP_280859.1. NC_002607.1.
WP_010903640.1. NC_002607.1.

3D structure databases

ProteinModelPortali Q9HN82.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 64091.VNG2210G.

Proteomic databases

PaxDbi Q9HN82.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG20339 ; AAG20339 ; VNG_2210G .
GeneIDi 1448641.
KEGGi hal:VNG2210G.

Phylogenomic databases

eggNOGi COG1676.
InParanoidi Q9HN82.
KOi K01170.
OMAi RGFYLSP.
PhylomeDBi Q9HN82.

Family and domain databases

Gene3Di 3.40.1350.10. 2 hits.
HAMAPi MF_01834. EndA_long.
InterProi IPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view ]
Pfami PF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view ]
SUPFAMi SSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsi TIGR00324. endA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiENDA_HALSA
AccessioniPrimary (citable) accession number: Q9HN82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3