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Q9HN82

- ENDA_HALSA

UniProt

Q9HN82 - ENDA_HALSA

Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity.By similarity

    Catalytic activityi

    PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei277 – 2771UniRule annotation
    Active sitei288 – 2881UniRule annotation
    Active sitei319 – 3191UniRule annotation

    GO - Molecular functioni

    1. lyase activity Source: UniProtKB-KW
    2. nucleic acid binding Source: InterPro
    3. tRNA-intron endonuclease activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA splicing, via endonucleolytic cleavage and ligation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    tRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
    Alternative name(s):
    tRNA-intron endonucleaseUniRule annotation
    Gene namesi
    Name:endAUniRule annotation
    Ordered Locus Names:VNG_2210G
    OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
    Taxonomic identifieri64091 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
    ProteomesiUP000000554: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 343343tRNA-splicing endonucleasePRO_0000109485Add
    BLAST

    Proteomic databases

    PaxDbiQ9HN82.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi64091.VNG2210G.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9HN82.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1676.
    KOiK01170.
    OMAiRGFYLSP.
    PhylomeDBiQ9HN82.

    Family and domain databases

    Gene3Di3.40.1350.10. 2 hits.
    HAMAPiMF_01834. EndA_long.
    InterProiIPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view]
    PfamiPF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view]
    SUPFAMiSSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsiTIGR00324. endA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9HN82-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD    50
    LDSVDGMGFR AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD 100
    GDFVVFPRGN TRRDGVVKHR VRVVDERTTL PVDGLSESVL AVVDEESEIT 150
    YLDIAPETPT GETTLDRPTD VPGVLLDDRV LVWEPPQRLH NAGFYGQPLG 200
    GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR LGEGEHFDCR 250
    LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH 300
    VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP 343
    Length:343
    Mass (Da):37,872
    Last modified:March 1, 2001 - v1
    Checksum:iE84404AC0C317CA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20339.1.
    PIRiG84371.
    RefSeqiNP_280859.1. NC_002607.1.
    WP_010903640.1. NC_002607.1.

    Genome annotation databases

    EnsemblBacteriaiAAG20339; AAG20339; VNG_2210G.
    GeneIDi1448641.
    KEGGihal:VNG2210G.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004437 Genomic DNA. Translation: AAG20339.1 .
    PIRi G84371.
    RefSeqi NP_280859.1. NC_002607.1.
    WP_010903640.1. NC_002607.1.

    3D structure databases

    ProteinModelPortali Q9HN82.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 64091.VNG2210G.

    Proteomic databases

    PaxDbi Q9HN82.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG20339 ; AAG20339 ; VNG_2210G .
    GeneIDi 1448641.
    KEGGi hal:VNG2210G.

    Phylogenomic databases

    eggNOGi COG1676.
    KOi K01170.
    OMAi RGFYLSP.
    PhylomeDBi Q9HN82.

    Family and domain databases

    Gene3Di 3.40.1350.10. 2 hits.
    HAMAPi MF_01834. EndA_long.
    InterProi IPR011856. tRNA_endonuc-like_dom.
    IPR006677. tRNA_intron_Endonuc_cat-like.
    IPR006678. tRNA_intron_Endonuc_N.
    IPR006676. tRNA_splic.
    IPR023516. tRNA_splic_arch_long.
    [Graphical view ]
    Pfami PF01974. tRNA_int_endo. 2 hits.
    PF02778. tRNA_int_endo_N. 2 hits.
    [Graphical view ]
    SUPFAMi SSF53032. SSF53032. 2 hits.
    SSF55267. SSF55267. 2 hits.
    TIGRFAMsi TIGR00324. endA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700922 / JCM 11081 / NRC-1.

    Entry informationi

    Entry nameiENDA_HALSA
    AccessioniPrimary (citable) accession number: Q9HN82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3