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Q9HN82 (ENDA_HALSA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-splicing endonuclease

EC=4.6.1.16
Alternative name(s):
tRNA-intron endonuclease
Gene names
Name:endA
Ordered Locus Names:VNG_2210G
OrganismHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]
Taxonomic identifier64091 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834

Catalytic activity

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus. HAMAP-Rule MF_01834

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01834

Sequence similarities

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA splicing, via endonucleolytic cleavage and ligation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionlyase activity

Inferred from electronic annotation. Source: UniProtKB-KW

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

tRNA-intron endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343tRNA-splicing endonuclease HAMAP-Rule MF_01834
PRO_0000109485

Sites

Active site2771 By similarity
Active site2881 By similarity
Active site3191 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9HN82 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: E84404AC0C317CA8

FASTA34337,872
        10         20         30         40         50         60 
MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD LDSVDGMGFR 

        70         80         90        100        110        120 
AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD GDFVVFPRGN TRRDGVVKHR 

       130        140        150        160        170        180 
VRVVDERTTL PVDGLSESVL AVVDEESEIT YLDIAPETPT GETTLDRPTD VPGVLLDDRV 

       190        200        210        220        230        240 
LVWEPPQRLH NAGFYGQPLG GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR 

       250        260        270        280        290        300 
LGEGEHFDCR LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH 

       310        320        330        340 
VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004437 Genomic DNA. Translation: AAG20339.1.
PIRG84371.
RefSeqNP_280859.1. NC_002607.1.

3D structure databases

ProteinModelPortalQ9HN82.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING64091.VNG2210G.

Proteomic databases

PaxDbQ9HN82.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG20339; AAG20339; VNG_2210G.
GeneID1448641.
KEGGhal:VNG2210G.

Phylogenomic databases

eggNOGCOG1676.
KOK01170.
OMARGFYLSP.
PhylomeDBQ9HN82.

Family and domain databases

Gene3D3.40.1350.10. 2 hits.
HAMAPMF_01834. EndA_long.
InterProIPR011856. tRNA_endonuc-like_dom.
IPR006677. tRNA_intron_Endonuc_cat-like.
IPR006678. tRNA_intron_Endonuc_N.
IPR006676. tRNA_splic.
IPR023516. tRNA_splic_arch_long.
[Graphical view]
PfamPF01974. tRNA_int_endo. 2 hits.
PF02778. tRNA_int_endo_N. 2 hits.
[Graphical view]
SUPFAMSSF53032. SSF53032. 2 hits.
SSF55267. SSF55267. 2 hits.
TIGRFAMsTIGR00324. endA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENDA_HALSA
AccessionPrimary (citable) accession number: Q9HN82
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: June 11, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families