Q9HN82 (ENDA_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-splicing endonuclease
EC=3.1.27.9

Alternative name(s):
tRNA-intron endonuclease

Gene names

Name:	endA
Ordered Locus Names:	VNG_2210G

Organism

Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium) [Reference proteome] [HAMAP]

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium › Halobacterium salinarum

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp By similarity. HAMAP-Rule MF_01834
Catalytic activity	Endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxy and 2',3'-cyclic phosphate termini, and specifically removing the intron. HAMAP-Rule MF_01834
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA splicing, via endonucleolytic cleavage and ligation Inferred from electronic annotation. Source: HAMAP
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro tRNA-intron endonuclease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	tRNA-splicing endonuclease HAMAP-Rule MF_01834		PRO_0000109485
Sites
	Active site	277	1	By similarity
	Active site	288	1	By similarity
	Active site	319	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HN82 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E84404AC0C317CA8	FASTA	343	37,872
	10 20 30 40 50 60 MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD LDSVDGMGFR 70 80 90 100 110 120 AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD GDFVVFPRGN TRRDGVVKHR 130 140 150 160 170 180 VRVVDERTTL PVDGLSESVL AVVDEESEIT YLDIAPETPT GETTLDRPTD VPGVLLDDRV 190 200 210 220 230 240 LVWEPPQRLH NAGFYGQPLG GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR 250 260 270 280 290 300 LGEGEHFDCR LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH 310 320 330 340 VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20339.1.
PIR	G84371.
RefSeq	NP_280859.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HN82.
ModBase	Search...
Protein-protein interaction databases
STRING	64091.VNG2210G.
Proteomic databases
PaxDb	Q9HN82.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG20339; AAG20339; VNG_2210G.
GeneID	1448641.
KEGG	hal:VNG2210G.
Phylogenomic databases
eggNOG	COG1676.
KO	K01170.
OMA	FELKYIV.
ProtClustDB	PRK09300.
Family and domain databases
Gene3D	3.40.1350.10. 2 hits.
HAMAP	MF_01834. EndA_long.
InterPro	IPR011856. tRNA_endonuc-like_dom. IPR006677. tRNA_intron_Endonuc_cat-like. IPR006678. tRNA_intron_Endonuc_N. IPR006676. tRNA_splic. IPR023516. tRNA_splic_arch_long. [Graphical view]
Pfam	PF01974. tRNA_int_endo. 2 hits. PF02778. tRNA_int_endo_N. 2 hits. [Graphical view]
SUPFAM	SSF53032. tRNA_int_endo_C. 2 hits. SSF55267. tRNA_int_endo_N. 2 hits.
TIGRFAMs	TIGR00324. endA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ENDA_HALSA

Accession

Primary (citable) accession number: Q9HN82

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program