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Protein

tRNA-splicing endonuclease

Gene

endA

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).By similarity

Catalytic activityi

PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei277UniRule annotation1
Active sitei288UniRule annotation1
Active sitei319UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processtRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-splicing endonucleaseUniRule annotation (EC:4.6.1.16UniRule annotation)
Alternative name(s):
tRNA-intron endonucleaseUniRule annotation
Gene namesi
Name:endAUniRule annotation
Ordered Locus Names:VNG_2210G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi
  • UP000000554 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094851 – 343tRNA-splicing endonucleaseAdd BLAST343

Proteomic databases

PaxDbiQ9HN82

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi64091.VNG2210G

Structurei

3D structure databases

ProteinModelPortaliQ9HN82
SMRiQ9HN82
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tRNA-intron endonuclease family. Archaeal long subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG01701 Archaea
COG1676 LUCA
InParanoidiQ9HN82
KOiK01170
OMAiGADFRTY
OrthoDBiPOG093Z0DMC
PhylomeDBiQ9HN82

Family and domain databases

Gene3Di3.40.1350.10, 2 hits
HAMAPiMF_01834 EndA_long, 1 hit
InterProiView protein in InterPro
IPR011856 tRNA_endonuc-like_dom_sf
IPR036167 tRNA_intron_Endo_cat-like_sf
IPR006677 tRNA_intron_Endonuc_cat-like
IPR006678 tRNA_intron_Endonuc_N
IPR036740 tRNA_intron_Endonuc_N_sf
IPR006676 tRNA_splic
IPR023516 tRNA_splic_arch_long
PANTHERiPTHR21227 PTHR21227, 2 hits
PfamiView protein in Pfam
PF01974 tRNA_int_endo, 1 hit
PF02778 tRNA_int_endo_N, 2 hits
SUPFAMiSSF53032 SSF53032, 2 hits
SSF55267 SSF55267, 2 hits
TIGRFAMsiTIGR00324 endA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HN82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGDLRGDTV HIGGDARQRF HDARGYGYPL GGNDIAVSLV EAAHLLFRGD
60 70 80 90 100
LDSVDGMGFR AFLTDREAGF AARFLVYVDL RDRGFYLVPD RDPWWRDPGD
110 120 130 140 150
GDFVVFPRGN TRRDGVVKHR VRVVDERTTL PVDGLSESVL AVVDEESEIT
160 170 180 190 200
YLDIAPETPT GETTLDRPTD VPGVLLDDRV LVWEPPQRLH NAGFYGQPLG
210 220 230 240 250
GRAADHDALQ LSLVEAAYLI DAGVLRLTDA TIDDVRARGR LGEGEHFDCR
260 270 280 290 300
LAVYRALRDA GAVPKTGFKF GADFRVYSAV SSVDDLGHSE LLVRVITDTH
310 320 330 340
VFSPGDLSLD VRLAHGVRKR MVFAATDDTD DTIRWLSVSR LTP
Length:343
Mass (Da):37,872
Last modified:March 1, 2001 - v1
Checksum:iE84404AC0C317CA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA Translation: AAG20339.1
PIRiG84371
RefSeqiWP_010903640.1, NC_002607.1

Genome annotation databases

EnsemblBacteriaiAAG20339; AAG20339; VNG_2210G
GeneIDi5954436
KEGGihal:VNG_2210G
PATRICifig|64091.14.peg.1699

Similar proteinsi

Entry informationi

Entry nameiENDA_HALSA
AccessioniPrimary (citable) accession number: Q9HN82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health