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Protein

Probable lipoyl synthase

Gene

lipA

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Probable lipoyl synthase (lipA)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi36Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi41Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi47Iron-sulfur 1 (4Fe-4S)UniRule annotation1
Metal bindingi66Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi70Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi73Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Probable lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Synonyms:lip
Ordered Locus Names:VNG_2216G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001023901 – 311Probable lipoyl synthaseAdd BLAST311

Proteomic databases

PaxDbiQ9HN78

Interactioni

Protein-protein interaction databases

STRINGi64091.VNG2216G

Structurei

3D structure databases

ProteinModelPortaliQ9HN78
SMRiQ9HN78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00660 Archaea
COG0320 LUCA
InParanoidiQ9HN78
KOiK03644
OMAiPYCDIDF
OrthoDBiPOG093Z05YO
PhylomeDBiQ9HN78

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9HN78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSRRKPEWL KMRPPSGERF TDIKETLRDN DLHTVCEEAS CPNMGECWSG
60 70 80 90 100
RDGPGTATFM LMGDRCSRGC NFCDVKTGGM EPLDPDEPAN VAESVAEIGL
110 120 130 140 150
DYVVLTSVDR DDLDDQGAGH FARTIREIKE RDPSILVEVL IPDFQGEKSL
160 170 180 190 200
VQKIIDAGPD VIAHNIETVA RRQVPVRDRR AGYEQSLSVL EYVTRESDIY
210 220 230 240 250
TKTSIMLGVG EYDHEVYQTL GDLREAGVDV VTLGQYLQPS RSHLDVADYV
260 270 280 290 300
HPQKFETWQR VAESEFGFLY CASGPMVRSS YKAGELFVDA VLREGKSVQE
310
ARRNARRAAS E
Length:311
Mass (Da):34,830
Last modified:March 1, 2001 - v1
Checksum:i8EE891416B65DB01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA Translation: AAG20343.1
PIRiC84372
RefSeqiWP_010903644.1, NC_002607.1

Genome annotation databases

EnsemblBacteriaiAAG20343; AAG20343; VNG_2216G
GeneIDi5953295
KEGGihal:VNG_2216G
PATRICifig|64091.14.peg.1703

Similar proteinsi

Entry informationi

Entry nameiLIPA_HALSA
AccessioniPrimary (citable) accession number: Q9HN78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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