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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei574 – 5741ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:VNG_2223G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 885885Leucine--tRNA ligasePRO_0000152129Add
BLAST

Proteomic databases

PaxDbiQ9HN72.

Interactioni

Protein-protein interaction databases

STRINGi64091.VNG2223G.

Structurei

3D structure databases

ProteinModelPortaliQ9HN72.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi571 – 5755"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ9HN72.
KOiK01869.
OMAiQSSWYFL.
PhylomeDBiQ9HN72.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9HN72-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEQATYDHH AIEQHWQREW DDADVFRVPD DADDPEYVLA MFPYTSGQLH
60 70 80 90 100
MGHVRNYAIT DAFARYRRMD GEDVLHPMGW DSFGLPAENA ANERDTNPRE
110 120 130 140 150
WTERCIDQMR DQFEALGFGY DWEREITTCD PDYYKWNQWL FTEFRDAGLV
160 170 180 190 200
DRRAATVNWC PSCETVLADE QVEGDDELCW RCDTPVTERD LDQWFFETTA
210 220 230 240 250
YADELLDGLD ELDGWPSNVR DMQRNWVGRT DGVEVPFTVH TPDGDEDVVA
260 270 280 290 300
FTTRVDTIHG ATYFALAPDH PLAEAAADRD DDVAHFVEEV ADPDGDEPQG
310 320 330 340 350
VETEFTATNP ATGAEIPVVV ADFVLSDVGT GALMAVPAHD DRDHEFAQAH
360 370 380 390 400
DLPVRQVVAP AGDEDADVEA AAYTADGVLV NAGDYTGLDS ETAREELTAD
410 420 430 440 450
IDGAASAVQY QLRDWGVSRQ RYWGTPIPIV HCESCGPVSV PDDDLPVELP
460 470 480 490 500
EFVHTTGNPL DAAEDWKQTT CPDCGAPAVR ETDTMDTFLD SSWYFLRFAS
510 520 530 540 550
PAFDDAPFDT QRANDWLPVD EYVGGDEHAV MHLLYSRFVT KAFADLDMLE
560 570 580 590 600
HREPFAGLTT QGMVLGEDGT KMSKSKDNGV APERIVDEYG ADTARLFTLR
610 620 630 640 650
AARPSKAFPW SEEGVRSSHT FLERLLSMAR AVNADATADG DLDPAAEYVA
660 670 680 690 700
RETAATVQAA TTHFDDMEFN RAVQAVDELV SLLVRYRDRD DAAPAVVARG
710 720 730 740 750
VTAAVKLLAP IAPHVAEECW TALGGDGFVA EAAWPTPDRD VSDHDRATSL
760 770 780 790 800
IEQTREDVRD IVDTAGIENP TGVDVVTAPE WMYDVLARAK AADGNVVGSV
810 820 830 840 850
MSDQSLQQHG EDAADYAKDL AAQAPAFPDV LGPDGERDAL GRAVWLLEAE
860 870 880
FDAPVRVLAA EDAADSVANK AEPGRPAIHV DEADD
Length:885
Mass (Da):97,527
Last modified:March 1, 2001 - v1
Checksum:iCA697263BE4D58F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20349.1.
PIRiA84373.
RefSeqiNP_280869.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG20349; AAG20349; VNG_2223G.
GeneIDi1448651.
KEGGihal:VNG2223G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004437 Genomic DNA. Translation: AAG20349.1.
PIRiA84373.
RefSeqiNP_280869.1. NC_002607.1.

3D structure databases

ProteinModelPortaliQ9HN72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64091.VNG2223G.

Proteomic databases

PaxDbiQ9HN72.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG20349; AAG20349; VNG_2223G.
GeneIDi1448651.
KEGGihal:VNG2223G.

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ9HN72.
KOiK01869.
OMAiQSSWYFL.
PhylomeDBiQ9HN72.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiSYL_HALSA
AccessioniPrimary (citable) accession number: Q9HN72
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2001
Last modified: March 4, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.