Reviewed, UniProtKB/Swiss-Prot Q9HN50 (SAHH_HALSA)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Synonyms:	achY
Ordered Locus Names:	VNG_2251G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000117002
Regions
	Region	184 – 353	170	NAD binding By similarity
Sites
	Binding site	132	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HN50-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6131D032241E1377	FASTA	427	45,907
	10 20 30 40 50 60 MTTAPAISSR LDDPESARET GRAKIDWAFE HMPILSALRE EFDANQPLAG ETIGMAMHVE 70 80 90 100 110 120 AKTAALVETM ADAGAEIAIT GCNPLSTHDG VSAALDAHES ITSYAERGAE GEAYYDAIDA 130 140 150 160 170 180 VLAHEPTVTV DDGGDLVFRV HEDHPELIDT IIGGTEETTT GVHRLRAMDD DDALEYPVFA 190 200 210 220 230 240 VNDTPMKRLF DNVHGTGESA LANIAMTTNL SWAGKDVVVA GYGDCGRGVA KKAAGQNANV 250 260 270 280 290 300 IVTEVEPRRA LEAHMEGYDV MPMAEAAEVG DVFLTTTGNK NVITRAHFER MDDGVVLANA 310 320 330 340 350 360 GHFDVEVNLD HLSELAVSER EAREGVREYE LADGRRLNVL AEGRLVNLAS PIGLGHPVGV 370 380 390 400 410 420 MDQSFGVQAV CVRELVANRE EYAAGVHNVP DELDIEIAEI KLAAEGVEYD ALTDDQAEYM DSWQHGT

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20371.1.
PIR	G84375.
RefSeq	NP_280891.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1448673.
GenomeReviews	Gene locus VNG_2251G in contig AE004437_GR.
KEGG	hal:VNG2251G.
NMPDR	fig|64091.1.peg.1917.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG352029.
OMA	GETNEEY.
Enzyme and pathway databases
BioCyc	HSP64091:VNG2251G-MONOMER.
BRENDA	3.3.1.1. 141893.
Family and domain databases
HAMAP	MF_00563_A. AdoHcyase_A. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HALSA

Accession

Primary (citable) accession number: Q9HN50

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)