Q9HN50 (SAHH_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosylhomocysteinase
EC=3.3.1.1

Alternative name(s):
S-adenosyl-L-homocysteine hydrolase
Short name=AdoHcyase

Gene names

Name:	ahcY
Synonyms:	achY
Ordered Locus Names:	VNG_2251G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May play a key role in the regulation of the intracellular concentration of adenosylhomocysteine By similarity. HAMAP MF_00563
Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000117002
Regions
	Nucleotide binding	158 – 160	3	NAD By similarity
	Nucleotide binding	221 – 226	6	NAD By similarity
	Nucleotide binding	300 – 302	3	NAD By similarity
Sites
	Binding site	132	1	Substrate By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	192	1	NAD By similarity
	Binding site	244	1	NAD By similarity
	Binding site	279	1	NAD By similarity
	Binding site	347	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HN50 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6131D032241E1377	FASTA	427	45,907
	10 20 30 40 50 60 MTTAPAISSR LDDPESARET GRAKIDWAFE HMPILSALRE EFDANQPLAG ETIGMAMHVE 70 80 90 100 110 120 AKTAALVETM ADAGAEIAIT GCNPLSTHDG VSAALDAHES ITSYAERGAE GEAYYDAIDA 130 140 150 160 170 180 VLAHEPTVTV DDGGDLVFRV HEDHPELIDT IIGGTEETTT GVHRLRAMDD DDALEYPVFA 190 200 210 220 230 240 VNDTPMKRLF DNVHGTGESA LANIAMTTNL SWAGKDVVVA GYGDCGRGVA KKAAGQNANV 250 260 270 280 290 300 IVTEVEPRRA LEAHMEGYDV MPMAEAAEVG DVFLTTTGNK NVITRAHFER MDDGVVLANA 310 320 330 340 350 360 GHFDVEVNLD HLSELAVSER EAREGVREYE LADGRRLNVL AEGRLVNLAS PIGLGHPVGV 370 380 390 400 410 420 MDQSFGVQAV CVRELVANRE EYAAGVHNVP DELDIEIAEI KLAAEGVEYD ALTDDQAEYM DSWQHGT

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20371.1.
PIR	G84375.
RefSeq	NP_280891.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HN50.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1448673.
GenomeReviews	Gene locus VNG_2251G in contig AE004437_GR.
KEGG	hal:VNG2251G.
NMPDR	fig|64091.1.peg.1917.
Phylogenomic databases
HOGENOM	HBG352029.
OMA	MEVYAWR.
PhylomeDB	Q9HN50.
ProtClustDB	PRK05476.
Enzyme and pathway databases
BioCyc	HSP64091:VNG2251G-MONOMER.
Family and domain databases
HAMAP	MF_00563. AdoHcyase. [Tree]
InterPro	IPR000043. Adenosylhomocysteinase. IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
KO	K01251.
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
SMART	SM00996. AdoHcyase. 1 hit. SM00997. AdoHcyase_NAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00936. AhcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_HALSA

Accession

Primary (citable) accession number: Q9HN50

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program