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Q9HMX8 (DP2L_HALSA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Cleaved into the following chain:

  1. Hsp-NRC1 polC intein
    Alternative name(s):
    Hsp-NRC1 pol2 intein
Gene names
Name:polC
Synonyms:polA2
Ordered Locus Names:VNG_2338G
OrganismHalobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifier64091 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length1370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324

Catalytic activity

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324

Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324

Subunit structure

Heterodimer of a large subunit and a small subunit By similarity.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation Potential. HAMAP MF_00324

Sequence similarities

Belongs to the archaeal DNA polymerase II family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 925925DNA polymerase II large subunit, 1st part Potential
PRO_0000007301
Chain926 – 1120195Hsp-NRC1 polC intein Potential
PRO_0000007302
Chain1121 – 1370250DNA polymerase II large subunit, 2nd part Potential
PRO_0000007303

Sequences

Sequence LengthMass (Da)Tools
Q9HMX8 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 07878AA9976790C9

FASTA1,370150,296
        10         20         30         40         50         60 
MRPTDETYFE TLETGLDDAF EVAEAARERG EDPTPNVEIP VAKDMADRVE NILGIDGVAE 

        70         80         90        100        110        120 
RVRDLDGEMS REEAALELVA DFVDGRVGDY DTDAGKIEGA VRTAVALLTE GVVAAPIEGI 

       130        140        150        160        170        180 
DRVEVNDNDD GTQYVAVYYA GPIRSAGGTA QALSVLVADY ARAMLGIDAF KPRDDEIERY 

       190        200        210        220        230        240 
AEEVDLYDSE TGLQYSPKDA ETTFITEHCP VMLDGEATGN EEVDGFRDLE RIDTNSPRGG 

       250        260        270        280        290        300 
MCLVLAEGIA LKAPKIQRYT RNLDEVAWPW LQDLIDGTIG ADDADEDTPD AGSDSDATDE 

       310        320        330        340        350        360 
GDAPSASTDA EEPPRAAPSD KFLRDLIAGR PVFGHPSKNG GFRLRYGRAR NHGNATAGVH 

       370        380        390        400        410        420 
PATMHLLDDF LATGTQIKTE RPGKAAGIVP VDSIEGPTVK LANGDVRHIN DPADALDVRN 

       430        440        450        460        470        480 
GVAEILDVGE YLVNYGEFVE NNHELAPASY APEWWIQDLD AAGADVQALR DSPYVDLTAP 

       490        500        510        520        530        540 
TADQAMAWAT DYDAPLHPAY TYLWHDIDVE QFRALADAVA DAHTDASDDD DRGVLVLDHT 

       550        560        570        580        590        600 
TTVRETLEAL LVTHHQGDDT IRVDDWLPLA RSLGVTESLD REWETLSEAA AEWPNAVRAV 

       610        620        630        640        650        660 
NEVAPFSVQE RAPTRIGNRM GRPEKSESRD LSPAVHTLFP IGDAGGSQRD VADAARYAPD 

       670        680        690        700        710        720 
MSDTPGEIPV RVGDRVCPSC DEHTYESRCP DCGDWTDPHY ECRDCGAVAT PDESGRVECP 

       730        740        750        760        770        780 
NCGRDLDNVT TQVIDINDEY HGALRAVGER ENAFDQLKGV KGLLSAEKTP EPMAKGVLRA 

       790        800        810        820        830        840 
KHDVTAFKDG TVRYDMTDLP VTAVTPAELD VTAGQFRELG YNQDIHGDPL EHDDQLVELR 

       850        860        870        880        890        900 
VQDVVLSDGA ADHMLKTADF VDDLLTQYYG LDAFYDLDDR DDLVGELVFG MAPHTSAAVV 

       910        920        930        940        950        960 
GRVAGFTSAS VGYAHPYFHA AKRRNCFHPE TNVWFRDESG EWHHDPIETL VEARLDPDTA 

       970        980        990       1000       1010       1020 
DEDDFGALVQ ALDGDVFVPS VTEDGEETLQ RVEAVSKHPA PDHLLAVETK RGRELTVTPD 

      1030       1040       1050       1060       1070       1080 
HSMRRWTGDG IERVDARELT AGDALPAPTQ VPGDGETATS ELRSESLDGT HPQRRFGDGG 

      1090       1100       1110       1120       1130       1140 
SVRTDEVVSV EPVRSSVDHT YSLTVAETNT LVANGLFTGQ CDGDEDCVML LMDGLINFSK 

      1150       1160       1170       1180       1190       1200 
SYLPDKRGGR MDAPLVMSSR IDPAEIDDEA HNIDIDREYP REFYEATREL ADPEDVADLI 

      1210       1220       1230       1240       1250       1260 
TLAESTVGTD EEYTGFGHTH ATSNIHLGPS LSAYKTLGSM MDKMDAQLEL ARKLRSVAET 

      1270       1280       1290       1300       1310       1320 
DVAERVIEYH FLPDLIGNLR AFSRQETRCL DCGEKYRRMP LSGDCRECGG RVNLTVHEGS 

      1330       1340       1350       1360       1370 
VNKYMDTAMR VATEYDCREY TKQRLEIMDR RLESVFEDDT NKQSGISDFM

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004437 Genomic DNA. Translation: AAG20443.1.
PIRG84384.
RefSeqNP_280963.1. NC_002607.1.

3D structure databases

ProteinModelPortalQ9HMX8.
ModBaseSearch...

Protein family/group databases

MEROPSN10.006.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1448745.
GenomeReviewsGene locus VNG_2338G in contig AE004437_GR.
KEGGhal:VNG2338G.
NMPDRfig|64091.1.peg.1990.

Phylogenomic databases

HOGENOMHBG497074.
OMANCDGDED.
ProtClustDBPRK14714.

Enzyme and pathway databases

BioCycHSP64091:VNG2338G-MONOMER.

Family and domain databases

HAMAPMF_00324. DNApol_II_L_arch.
[Tree]
InterProIPR003586. Hedgehog_hint_C.
IPR003587. Hedgehog_hint_N.
IPR006141. Intein_splice_site.
IPR004475. PolC_DP2.
IPR016033. PolC_DP2_N.
[Graphical view]
KOK02322.
PfamPF03833. PolC_DP2. 1 hit.
[Graphical view]
PIRSFPIRSF016275. PolC_DP2. 1 hit.
SMARTSM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
TIGRFAMsTIGR01443. Intein_Cterm. 1 hit.
TIGR01445. Intein_Nterm. 1 hit.
TIGR00354. PolC. 1 hit.
PROSITEPS50818. INTEIN_C_TER. False negative.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDP2L_HALSA
AccessionPrimary (citable) accession number: Q9HMX8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: November 16, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

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