Reviewed, UniProtKB/Swiss-Prot Q9HMH9 (PYRC_HALSA)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	VNG_2533G

Organism

Halobacterium salinarium (Halobacterium halobium) [Complete proteome] [HAMAP]

Taxonomic identifier

2242 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00220
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Dihydroorotase HAMAP MF_00220		PRO_0000147268
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	172	1	Zinc 2 By similarity
	Metal binding	223	1	Zinc 2 By similarity
	Metal binding	291	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HMH9-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C055ADECC6E34125	FASTA	422	44,817
	10 20 30 40 50 60 MRVIRDAVLA DGRVRDIRLD GERIDAVGTA LDGELFVDAN ADYRVLPGMI DAHVHFRQPG 70 80 90 100 110 120 FEHKETWASG SRSAAAGGVT TVVDQPNTAP PTVTGDAVDE KAAHAAADSV VDWGVNGGVT 130 140 150 160 170 180 GEWDPASLFD RSLFALGEVF LADSTGDMGI DAALFRDACQ RAASEDVVVT VHAEDADRFD 190 200 210 220 230 240 TAAKSRSDAD AWSAYRTPEA EAAAVERAVE VGTEAGATIH IAHTSTPEGV DAAAAGGATC 250 260 270 280 290 300 EATPHHLFLS RDDLDDLGTF GRMNPPLRSD PRREALFERL ADGRIDVVAT DHAPHTRAEK 310 320 330 340 350 360 AADIWDAPSG VPGVETALPL LLGAAHRGEL SYERVRDVTA ANPADVFGLE RKGHIAAGRD 370 380 390 400 410 420 ADLVLVDPDD AREIHGDDLH SNCEWTPFEG HVGVFPAMTL VRGTTVWDGD TVSAFDGRNV RQ

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004437 Genomic DNA. Translation: AAG20592.1.
PIR	D84403.
RefSeq	NP_281112.1.
3D structure databases
HSSP	HSSP built from PDB template 1GKR based on UniProtKB P81006.
ModBase	Search...
Genome annotation databases
GeneID	1448897.
GenomeReviews	Gene locus VNG_2533G in contig AE004437_GR.
KEGG	hal:VNG2533G.
NMPDR	fig|64091.1.peg.2139.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9HMH9.
OMA	Q9HMH9. HSKAGWT.
Enzyme and pathway databases
BioCyc	HSP64091:VNG2533G-MON.
BRENDA	3.5.2.3. 141893.
Family and domain databases
HAMAP	MF_00220. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOmult. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
ProDom	PD000518. DHOase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00857. pyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HALSA

Accession

Primary (citable) accession number: Q9HMH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)