Q9HMH9 (PYRC_HALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	VNG_2533G

Organism

Halobacterium salinarium (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)

Taxonomic identifier

64091 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Halobacteria › Halobacteriales › Halobacteriaceae › Halobacterium

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_A
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_A
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_A
Subunit structure	Homodimer By similarity. HAMAP MF_00220_A
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Dihydroorotase HAMAP MF_00220_A		PRO_0000147268
Sites
	Metal binding	53	1	Zinc 1 By similarity
	Metal binding	55	1	Zinc 1 By similarity
	Metal binding	172	1	Zinc 2 By similarity
	Metal binding	223	1	Zinc 2 By similarity
	Metal binding	291	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9HMH9 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C055ADECC6E34125	FASTA	422	44,817
	10 20 30 40 50 60 MRVIRDAVLA DGRVRDIRLD GERIDAVGTA LDGELFVDAN ADYRVLPGMI DAHVHFRQPG 70 80 90 100 110 120 FEHKETWASG SRSAAAGGVT TVVDQPNTAP PTVTGDAVDE KAAHAAADSV VDWGVNGGVT 130 140 150 160 170 180 GEWDPASLFD RSLFALGEVF LADSTGDMGI DAALFRDACQ RAASEDVVVT VHAEDADRFD 190 200 210 220 230 240 TAAKSRSDAD AWSAYRTPEA EAAAVERAVE VGTEAGATIH IAHTSTPEGV DAAAAGGATC 250 260 270 280 290 300 EATPHHLFLS RDDLDDLGTF GRMNPPLRSD PRREALFERL ADGRIDVVAT DHAPHTRAEK 310 320 330 340 350 360 AADIWDAPSG VPGVETALPL LLGAAHRGEL SYERVRDVTA ANPADVFGLE RKGHIAAGRD 370 380 390 400 410 420 ADLVLVDPDD AREIHGDDLH SNCEWTPFEG HVGVFPAMTL VRGTTVWDGD TVSAFDGRNV RQ

References

[1]

"Genome sequence of Halobacterium species NRC-1." Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D., Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D., Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K. , Cruz R., Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P., Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M., Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J., Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L., DasSarma S. Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000) [PubMed: 11016950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700922 / JCM 11081 / NRC-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004437 Genomic DNA. Translation: AAG20592.1.
PIR	D84403.
RefSeq	NP_281112.1. NC_002607.1.
3D structure databases
ProteinModelPortal	Q9HMH9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1448897.
GenomeReviews	Gene locus VNG_2533G in contig AE004437_GR.
KEGG	hal:VNG2533G.
NMPDR	fig|64091.1.peg.2139.
Phylogenomic databases
HOGENOM	HBG724623.
OMA	GKFNPPL.
PhylomeDB	Q9HMH9.
ProtClustDB	PRK02382.
Enzyme and pathway databases
BioCyc	HSP64091:VNG2533G-MONOMER.
Family and domain databases
HAMAP	MF_00220_A. PyrC_type2_A. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
TIGRFAMs	TIGR00857. PyrC_multi. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_HALSA

Accession

Primary (citable) accession number: Q9HMH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program